Lactiplantibacillus plantarum associated to fermented foods: in vitro evidences for its beneficial role in ameliorating intestinal inflammation

Fermented food microbes have recently recovered scientific interest for their health-promoting potential. Among them, Lactiplantibacillus (Lpb.) plantarum strains, with a long history as starter cultures in the production of a wide variety of fermented foods, showed the potential to affect host health in several in vitro and in vivo studies. Our study was aimed to investigate selected Lpb. plantarum strains, isolated from fermented foods, to face oxidative stress and related inflammatory damage at intestinal level, in order to be considered a promising strategy in intestinal inflammatory diseases. For this purpose, we examined food- and human- associated Lpb. plantarum strains for their in vitro capacity to tolerate oxidative stress as well as for their antioxidant potential by three different chemical assays (DPPH, ABTS and FRAP). In addition, the specific ability of each strain to modulate reactive oxygen species (ROS) levels in response to either oxidative or inflammatory stress and to reduce IL-17A, IL-17F and IL-23 release in an inflamed intestinal cell model was investigated. Overall, the results show that Lpb. plantarum endure high levels of induced oxidative stress through partially neutralizing ROS, whereas they elicit their production when co‑cultured with normal mucosa intestinal cells (NCM460). Moreover, pre‑treatment with food‑borne Lpb. plantarum significantly reduces pro ‑inflammatory cytokines IL‑17F and IL‑23 levels in inflamed NCM460 cells. Both IL-23 and IL-17 are pro-inflammatory cytokines with an active role in the pathophysiology of chronic inflammatory disorders, thus targeting the IL23/IL17 axis could be a considerable way to treat intestinal inflammatory diseases. Our results suggest that food‑vehicled Lpb. plantarum strains might reduce inflammatory response in intestinal cells by directly modulating local ROS production and by triggering the IL‑23/IL‑17 axis with future perspectives on health benefits derived by the consumption of functional foods enriched with selected strains

Psychoeducation as a strategy to improve family support perceived from patient with alcohol dependence and personality disorder

The aim of this study was to prospectively analyze the impact of psychoeducation with relatives of addicted patients with personality disorders. We measured the impact of the psychoeducational intervention using a self-report questionnaire designed to assess perceived familiar support. The sample was composed by 37 patients with alcohol dependence following outpatient treatment. They underwent a cognitive-behavioral therapy relapse prevention program especially tailored for addicted individuals with comorbid personality disorders. 56.8% of patients followed individual therapy, and 43.2% followed group therapy. The relatives of the subgroup patients following group therapy simultaneously received the psychoeducational intervention. The analyses of the familiar support questionnaire across treatment showed a significant difference between groups as a function of treatment modality. Patients whose relatives followed the psychoeducational intervention had greater perception of familiar support throughout the treatment process

Comparative Genomics of Lactiplantibacillus plantarum: Insights Into Probiotic Markers in Strains Isolated From the Human Gastrointestinal Tract and Fermented Foods

Lactiplantibacillus (Lpb.) plantarum is a versatile species commonly found in a wide variety of ecological niches including dairy products and vegetables, while it may also occur as a natural inhabitant of the human gastrointestinal tract. Although Lpb. plantarum strains have been suggested to exert beneficial properties on their host, the precise mechanisms underlying these microbe-host interactions are still obscure. In this context, the genome-scale in silico analysis of putative probiotic bacteria represents a bottom-up approach to identify probiotic biomarkers, predict desirable functional properties, and identify potentially detrimental antibiotic resistance genes. In this study, we characterized the bacterial genomes of three Lpb. plantarum strains isolated from three distinct environments [strain IMC513 (from the human GIT), C904 (from table olives), and LT52 (from raw-milk cheese)]. A whole-genome sequencing was performed combining Illumina short reads with Oxford Nanopore long reads. The phylogenomic analyses suggested the highest relatedness between IMC513 and C904 strains which were both clade 4 strains, with LT52 positioned within clade 5 within the Lpb. plantarum species. The comparative genome analysis performed across several Lpb. plantarum representatives highlighted the genes involved in the key metabolic pathways as well as those encoding potential probiotic features in these new isolates. In particular, our strains varied significantly in genes encoding exopolysaccharide biosynthesis and in contrast to strains IMC513 and C904, the LT52 strain does not encode a Mannose-binding adhesion protein. The LT52 strain is also deficient in genes encoding complete pentose phosphate and the Embden-Meyerhof pathways. Finally, analyses using the CARD and ResFinder databases revealed that none of the strains encode known antibiotic resistance loci. Ultimately, the results provide better insights into the probiotic potential and safety of these three strains and indicate avenues for further mechanistic studies using these isolates

Prefrontal gray matter and motivation for treatment in cocaine dependent individuals with and without personality disorders

Addiction treatment is a long-term goal and therefore prefrontal–striatal regions regulating goal-directed behavior are to be associated with individual differences on treatment motivation. We aimed at examining the association between gray matter volumes in prefrontal cortices and striatum and readiness to change at treatment onset in cocaine users with and without personality disorders. Participants included 17 cocaine users without psychiatric comorbidities, 17 cocaine users with Cluster B disorders, and 12 cocaine users with Cluster C disorders. They completed the University of Rhode Island Change Assessment Scale, which measures four stages of treatment change (precontemplation, contemplation, action, and maintenance) and overall readiness to change, and were scanned in a 3 T MRI scanner. We defined three regions of interest (ROIs): the ventromedial prefrontal cortex (including medial orbitofrontal cortex and subgenual and rostral anterior cingulate cortex), the dorsomedial prefrontal cortex (i.e., superior medial frontal cortex), and the neostriatum (caudate and putamen). We found that readiness to change correlated with different aspects of ventromedial prefrontal gray matter as a function of diagnosis. In cocaine users with Cluster C comorbidities, readiness to change positively correlated with gyrus rectus gray matter, whereas in cocaine users without comorbidities it negatively correlated with rostral anterior cingulate cortex gray matter. Moreover, maintenance scores positively correlated with dorsomedial prefrontal gray matter in cocaine users with Cluster C comorbidities, but negatively correlated with this region in cocaine users with Cluster B and cocaine users without comorbidities. Maintenance scores also negatively correlated with dorsal striatum gray matter in cocaine users with Cluster C comorbidities. We conclude that the link between prefrontal–striatal gray matter and treatment motivation is modulated by co-existence of personality disorders

Critical review of the use of ilion for gender determination in skeletal remains of subadult individuals by morphometric techniques

La búsqueda de características morfológicas determinantes del sexoen restos esqueléticos de individuos subadultos ha sido una de lasproblemáticas recientes tratadas con mayor interés por los antropólogosfísicos, forenses y bioarqueólogos. En este trabajo se revisan lospuntos tomados como referencia para la determinación de sexo sobreimágenes digitales de ilion infantil. Se describen las configuracionesde landmarks y semilandmarks que representan diferentes regionesanatómicas del ilion y, analizando los datos morfológicos mediantetécnicas de morfometría geométrica, se evalúan las diferencias debidasal sexo en cada una de ellas. Para la realización de este trabajose utilizaron fotografías digitales del hueso ilíaco de 216 individuosde sexo conocido, con edades comprendidas entre el periodo fetal ylos 16 años posteriores al nacimiento pertenecientes a colecciones osteológicasdocumentadas provenientes de Granada (España), Coimbra(Portugal), Lisboa (Portugal) y La Plata (Argentina). El análisis delas imágenes permitió confirmar la existencia de diferencias asociadascon el sexo en la morfología del ilion e identificar configuraciones depuntos que pueden reconocerse para el estudio de la variación duranteel desarrollo y la expresión del dimorfismo sexual. Se encontró que el borde de la carilla auricular no evidencia diferencias dimórficasidentificables en todo el período considerado. En cambio, la formadel contorno del ilion y de la escotadura ciática mayor arrojaron resultadossignificativos en la evaluación del dimorfismo. Se discutenlas dificultades encontradas en la observación de los landmarks y sepropone que la generación de definiciones específicas para diferentesgrupos etarios serían útiles para la comprensión de la variación morfológicahaciendo comparables los nuevos resultados.Fil: Garcia Mancuso, Rocio. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Petrone, Selene. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Salceda, Susana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Antropología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gonzalez, Paula Natalia. Universidad Nacional Arturo Jauretche. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos; Argentin