3,947 research outputs found
Phylogenetic inferences of Atelinae (Platyrrhini) based on multi-directional chromosome painting in Brachyteles arachnoides, Ateles paniscus paniscus and Ateles b. marginatus
We performed multi-directional chromosome painting in a comparative cytogenetic study of the three Atelinae species Brachyteles arachnoides, Ateles paniscus paniscus and Ateles belzebuth marginatus, in order to reconstruct phylogenetic relationships within this Platyrrhini subfamily. Comparative chromosome maps between these species were established by multi-color fluorescence in situ hybridization ( FISH) employing human, Saguinus oedipus and Lagothrix lagothricha chromosome-specific probes. The three species included in this study and four previously analyzed species from all four Atelinae genera were subjected to a phylogenetic analysis on the basis of a data matrix comprised of 82 discrete chromosome characters. The results confirmed that Atelinae represent a monophyletic clade with a putative ancestral karyotype of 2n = 62 chromosomes. Phylogenetic analysis revealed an evolutionary branching sequence \{Alouatta \{Brachyteles \{Lagothrix and Ateles\}\}\} in Atelinae and \{Ateles belzebuth marginatus \{Ateles paniscus paniscus \{Ateles belzebuth hybridus and Ateles geoffroyi\}\}\} in genus Ateles. The chromosomal data support a re-evaluation of the taxonomic status of Ateles b. hybridus. Copyright (C) 2005 S. Karger AG, Basel
Global P- and S-wave tomography from finite-frequency traveltime residual measurements
There are two main ways to improve the resolution of tomographic images: building larger and higher quality datasets, and using better imaging theories, such as finite frequency theory or waveform inversion, instead of the more commonly used ray theory. A first step toward improving the resolution of global tomographic models is thus to construct a seismological database adapted to finite-frequency tomography or waveform inversion. We present a global dataset of finite frequency travel time residuals. These measurements were obtained by cross-correlating observed and synthetic waveforms in several frequency bands. We exploit seismograms at significantly shorter periods than in other recent comparable works. We compute Green's functions with the GEMINI method and convolve them with high-frequency source-time functions. These source-time functions are inverted, along with focal depths, with a simulated annealing approach. We measure travel time residuals on different P and S phases. We check the quality of our global database by performing preliminary tomographic inversions using ray theory. We use the subset of measurements performed in the 0.0725-Hz frequency range, combined to ISC travel times, to build tomographic models for both P and S velocity anomalies. Resulting models are presented and compared to recently published global tomographic models, and some implications for finite frequency effects and physical properties of the deep Earth are discussed
Tuning of Human Modulation Filters Is Carrier-Frequency Dependent
Licensed under the Creative Commons Attribution License
Single-Molecule Super-Resolution Imaging of T-Cell Plasma Membrane CD4 Redistribution upon HIV-1 Binding
The first step of cellular entry for the human immunodeficiency virus type-1 (HIV-1) occurs
through the binding of its envelope protein (Env) with the plasma membrane receptor CD4 and
co-receptor CCR5 or CXCR4 on susceptible cells, primarily CD4+ T cells and macrophages. Although
there is considerable knowledge of the molecular interactions between Env and host cell receptors
that lead to successful fusion, the precise way in which HIV-1 receptors redistribute to sites of
virus binding at the nanoscale remains unknown. Here, we quantitatively examine changes in the
nanoscale organisation of CD4 on the surface of CD4+ T cells following HIV-1 binding. Using singlemolecule super-resolution imaging, we show that CD4 molecules are distributed mostly as either
individual molecules or small clusters of up to 4 molecules. Following virus binding, we observe
a local 3-to-10-fold increase in cluster diameter and molecule number for virus-associated CD4
clusters. Moreover, a similar but smaller magnitude reorganisation of CD4 was also observed with
recombinant gp120. For one of the first times, our results quantify the nanoscale CD4 reorganisation
triggered by HIV-1 on host CD4+ T cells. Our quantitative approach provides a robust methodology
for characterising the nanoscale organisation of plasma membrane receptors in general with the
potential to link spatial organisation to function
Tagging frogs with passive integrated transponders causes disruption of the cutaneous bacterial community and proliferation of opportunistic fungi
Symbiotic bacterial communities play a key role in protecting amphibians from infectious diseases including chytridiomycosis,caused by the pathogenic fungus Batrachochytrium dendrobatidis. Events that lead to the disruption of the bacterial community may have implications for the susceptibility of amphibians to such diseases. Amphibians are often marked both in the wild and
in captivity for a variety of reasons, and although existing literature indicates that marking techniques have few negative effects, the response of cutaneous microbial communities has not yet been investigated. Here we determine the effects of passive integrated
transponder (PIT) tagging on culturable cutaneous microbial communities of captive Morelet’s tree frogs (Agalychnis
moreletii) and assess the isolated bacterial strains for anti-B. dendrobatidis activity in vitro. We find that PIT tagging causes a major disruption to the bacterial community associated with the skin of frogs (∼12-fold increase in abundance), as well as a concurrent
proliferation in resident fungi (up to ∼200-fold increase). Handling also caused a disruption the bacterial community,
although to a lesser extent than PIT tagging. However, the effects of both tagging and handling were temporary, and after 2 weeks, the bacterial communities were similar to their original compositions. We also identify two bacterial strains that inhibit B. dendrobatidis, one of which increased in abundance on PIT-tagged frogs at 1 day postmarking, while the other was unaffected.
These results show that PIT tagging has previously unobserved consequences for cutaneous microbial communities of frogs and may be particularly relevant for studies that intend to use PIT tagging to identify individuals involved in trials to develop probiotic treatments
Chemical diplomacy in male tilapia: urinary signal increases sex hormone and decreases aggression
Androgens, namely 11-ketotestosterone (11KT), have a central role in male fish reproductive physiology and are thought to be involved in both aggression and social signalling. Aggressive encounters occur frequently in social species, and fights may cause energy depletion, injury and loss of social status. Signalling for social dominance and fighting ability in an agonistic context can minimize these costs. Here, we test the hypothesis of a 'chemical diplomacy' mechanism through urinary signals that avoids aggression and evokes an androgen response in receiver males of Mozambique tilapia (Oreochromis mossambicus). We show a decoupling between aggression and the androgen response; males fighting their mirror image experience an unresolved interaction and a severe drop in urinary 11KT. However, if concurrently exposed to dominant male urine, aggression drops but urinary 11KT levels remain high. Furthermore, 11KT increases in males exposed to dominant male urine in the absence of a visual stimulus. The use of a urinary signal to lower aggression may be an adaptive mechanism to resolve disputes and avoid the costs of fighting. As dominance is linked to nest building and mating with females, the 11KT response of subordinate males suggests chemical eavesdropping, possibly in preparation for parasitic fertilizations.info:eu-repo/semantics/publishedVersio
Pneumocystis primary infection in non-immunosuppressed infants in Lima, Peru
Objectives:
To provide original data on Pneumocystis primary infection in non-immunosuppressed infants from Peru. /
Methods:
A cross sectional study was performed. Infants less than seven months old, without any underlying medical conditions attending the “well baby” outpatient clinic at one hospital in Lima, Peru were prospectively enrolled during a 15-month period from November 2016 to February 2018. All had a nasopharyngeal aspirate (NPA) for detection of P. jirovecii DNA using a PCR assay, regardless of respiratory symptoms. P. jirovecii DNA detection was considered to represent pulmonary colonization contemporaneous with Pneumocystis primary infection. Associations between infants’ clinical and demographic characteristics and results of P. jirovecii DNA detection were analyzed. /
Results:
P. jirovecii DNA was detected in 45 of 146 infants (30.8%) and detection was not associated with concurrent respiratory symptoms in 40 of 45 infants. Infants with P. jirovecii had a lower mean age when compared to infants not colonized (p <0.05). The highest frequency of P. jirovecii was observed in 2-3-month-old infants (p < 0.01) and in the cooler winter and spring seasons (p <0.01). Multivariable analysis showed that infants living in a home with ≤ 1 bedroom were more likely to be colonized; Odds Ratio =3.03 (95%CI 1.31-7.00; p =0.01). /
Conclusion:
Pneumocystis primary infection in this single site in Lima, Peru, was most frequently observed in 2-3-month-old infants, in winter and spring seasons, and with higher detection rates being associated with household conditions favoring close inter-individual contacts and potential transmission of P. jirovecii
Aerothermodynamic Analysis of a Reentry Brazilian Satellite
This work deals with a computational investigation on the small ballistic
reentry Brazilian vehicle SARA (acronyms for SAt\'elite de Reentrada
Atmosf\'erica). Hypersonic flows over the vehicle SARA at zero-degree angle of
attack in a chemical equilibrium and thermal non-equilibrium are modeled by the
Direct Simulation Monte Carlo (DSMC) method, which has become the main
technique for studying complex multidimensional rarefied flows, and that
properly accounts for the non-equilibrium aspects of the flows. The emphasis of
this paper is to examine the behavior of the primary properties during the high
altitude portion of SARA reentry. In this way, velocity, density, pressure and
temperature field are investigated for altitudes of 100, 95, 90, 85 and 80 km.
In addition, comparisons based on geometry are made between axisymmetric and
planar two-dimensional configurations. Some significant differences between
these configurations were noted on the flowfield structure in the reentry
trajectory. The analysis showed that the flow disturbances have different
influence on velocity, density, pressure and temperature along the stagnation
streamline ahead of the capsule nose. It was found that the stagnation region
is a thermally stressed zone. It was also found that the stagnation region is a
zone of strong compression, high wall pressure. Wall pressure distributions are
compared with those of available experimental data and good agreement is found
along the spherical nose for the altitude range investigated.Comment: The paper will be published in Vol. 42 of the Brazilian Journal of
Physic
Impact of metabolic comorbidity on the association between body mass index and heatlh-related quality of life: a Scotland-wide cross-sectional study of 5,608 participants
<p/>Background: The prevalence of obesity is rising in Scotland and globally. Overall, obesity is associated with increased morbidity, mortality and reduced health-related quality of life. Studies suggest that "healthy obesity" (obesity without metabolic comorbidity) may not be associated with morbidity or mortality. Its impact on health-related quality of life is unknown.
<p/>Methods: We extracted data from the Scottish Health Survey on self-reported health-related quality of life, body mass index (BMI), demographic information and comorbidity. SF-12 responses were converted into an overall health utility score. Linear regression analyses were used to explore the association between BMI and health utility, stratified by the presence or absence of metabolic comorbidity (diabetes, hypertension, hypercholesterolemia or cardiovascular disease), and adjusted for potential confounders (age, sex and deprivation quintile).
<p/>Results: Of the 5,608 individuals, 3,744 (66.8%) were either overweight or obese and 921 (16.4%) had metabolic comorbidity. There was an inverted U-shaped relationship whereby health utility was highest among overweight individuals and fell with increasing BMI. There was a significant interaction with metabolic comorbidity (p = 0.007). Individuals with metabolic comorbidty had lower utility scores and a steeper decline in utility with increasing BMI (morbidly obese, adjusted coefficient: -0.064, 95% CI -0.115, -0.012, p = 0.015 for metabolic comorbidity versus -0.042, 95% CI -0.067, -0.018, p = 0.001 for no metabolic comorbidity).
<p/>Conclusions: The adverse impact of obesity on health-related quality of life is greater among individuals with metabolic comorbidity. However, increased BMI is associated with reduced health-related quality of life even in the absence of metabolic comorbidity, casting doubt on the notion of "healthy obesity"
Species-level divergences in multiple functional traits between the two endemic subspecies of Blue Chaffinches Fringilla teydea in Canary Islands
Background: One of the biggest challenges in avian taxonomy is the delimitation of allopatric species because their reproductive incompatibility cannot be directly studied in the wild. Instead, reproductive incompatibility has to be inferred from multiple, divergent character sets that indicate a low likelihood of allopatric populations amalgamating upon secondary contact. A set of quantitative criteria for species delimitation has been developed for avian taxonomy.
Results: Here, we report a broad multi-trait comparison of the two insular subspecies of the Blue Chaffinch Fringilla teydea, endemic to the pine forests of Tenerife (ssp. teydea) and Gran Canaria (ssp. polatzeki) in the Canary Islands. We found that the two taxa were reciprocally monophyletic in their whole mitogenomes and two Z chromosome introns. The genetic distance in mitogenomes indicates around 1 Mya of allopatric evolution. There were diagnostic differences in body morphometrics, song and plumage reflectance spectra, whose combined divergence score (=11) exceeds the threshold level (=7) set for species delimitation by Tobias et al. (Ibis 152:724–746, 2010). Moreover, we found a marked divergence in sperm lengths with little range overlap. Relatively long sperm with low intra- and intermale CV compared to other passerines suggest a mating system with high levels of sperm competition (extrapair paternity) in these taxa.
Conclusion: The large and diagnostic divergences in multiple functional traits qualify for species rank, i.e., Tenerife Blue Chaffinch (Fringilla teydea) and Gran Canaria Blue Chaffinch (Fringilla polatzeki). We encourage a wider use of sperm traits in avian taxonomy because sperm divergences might signal reproductive incompatibility at the postcopulatory prezygotic stage, especially in species with sperm competition
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