424 research outputs found

    Comedouros para suínos em crescimento e terminação.

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    Detection of Leishmania infantum by PCR, serology and cellular immune response in a cohort study of Brazilian dogs

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    The sensitivity and specificity of PCR, serology (ELISA) and lymphoproliferative response to Leishmania antigen for the detection of Leishmania infantum infection were evaluated in a cohort of 126 dogs exposed to natural infection in Brazil. For PCR, Leishmania DNA from bone-marrow was amplified with both minicircle and ribosomal primers. The infection status and time of infection of each dog were estimated from longitudinal data. The sensitivity of PCR in parasite-positive samples was 98%. However, the overall sensitivity of PCR in post-infection samples, from dogs with confirmed infection, was only 68%. The sensitivity of PCR varied during the course of infection, being highest (78–88%) 0–135 days post-infection and declining to around 50% after 300 days. The sensitivity of PCR also varied between dogs, and was highest in sick dogs. The sensitivity of serology was similar in parasite-positive (84%), PCR-positive (86%) and post-infection (88%) samples. The sensitivity of serology varied during the course of infection, being lowest at the time of infection and high (93–100%) thereafter. Problems in determining the specificity of serology are discussed. The sensitivity and specificity of cellular responsiveness were low. These data suggest that PCR is most useful in detecting active or symptomatic infection, and that serology can be a more sensitive technique for the detection of all infected dogs

    Synchrotron powder diffraction study of cements pastes

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     Knowledge of the degree of hydration of cement pastes is critical for determining properties such as the durability of concrete. As part of an integrated study on the prediction of chloride ingress in reinforced concrete, synchrotron Xray powder diffraction was used to estimate the degree of hydration of cement pastes. While for the past 20 years the composition of Portland cement has been determined by Rietveld analysis of X-ray diffraction, nevertheless there are a number of factors, including the amorphous content of the cement and relative proportion of mineral polymorphs present in the initial clinker, whose impact on the analysis are still not completely understood. Analysis of the resulting diffraction patterns indicated enhanced identification of polymorphs of alite, belite, ferrite and aluminate, which are present in the initial unhydrated cement and clinker, as well as improved quantification of hydrated crystalline phases such as calcium hydroxide and ettringite, which are key phases determining the speed of the chemical reactions in cement. In this paper we describe the experience that we have gained in the determination of the degree of hydration of cement pastes. We detail the standards and precautions that we took to characterize production cements and their hydration products

    Real-time evaluation of two light delivery systems for photodynamic disinfection of Candida albicans biofilm in curved root canals

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    Antimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and investigated the microbial reduction produced when different light delivery methods are employed. Each light delivery method was evaluated in respect to the light distribution provided inside curved root canals. After conventional endodontic preparation, teeth were sterilized before canals were contaminated by a bioluminescent strain of C. albicans (CEC789). Methylene blue (90 ÎŒM) was introduced into the canals and then irradiated (λ = 660 nm, P = 100 mW, beam diameter = 2 mm) with laser tip either in contact with pulp chamber or within the canal using an optical diffuser fiber. Light distribution was evaluated by CCD camera, and microbial reduction was monitored through bioluminescence imaging. Our findings demonstrated that the bioluminescent C. albicans biofilm model had good reproducibility and uniformity. Light distribution in dental tissue was markedly dependent on the light delivery system, and this strategy was directly related to microbial destruction. Both light delivery systems performed significant fungal inactivation. However, when irradiation was performed with optical diffuser fiber, microbial burden reduction was nearly 100 times more effective. Bioluminescence is an interesting real-time analysis to endodontic C. albicans biofilm inactivation. APDT showed to be an effective way to inactivate C. albicans biofilms. Diffuser fibers provided optimized light distribution inside curved root canals and significantly increased APDT efficiency.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grant 2010/13313-9)Conselho Nacional de Pesquisas (Brazil) Conselho Nacional de Desenvolvimento Cientifico e TecnologicoNational Institute of Mental Health (U.S.) (NIH R01AI050875

    Produção de milho em um sistema agropastoril, na região semi-årido do Nordeste do Brasil.

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    0 estudo foi conduzido em um sistema agropastoril na Embrapa Caprinos em Sobral, CE, Brasil, no periodo de 1998 a 2002. Objetivou-se, no presente trabalho, avaliar a sustentabilidade da produçao de milho, atraves da incorporaçao da folhagem da leucena, na forma de adubaçao verde. Foram avaliados tres sistemas de produçao, 0 agropastoril, 0 tradicional e 0 sistema pilato. 0 delineamento experimental utilizado foi 0 inteiramente casualizado, com tres tratamentos e cinco repetiçoes. Houve efeito (P0,05) dos sistemas agropastoril e tradicional nas produçoes de milho, mas estes diferiram (P<0,05) do sistema pilato. As produçoes medias de milho foram de 1.299, 1.360 e 542 kg/ha/ano para os sistemas agropastoril, tradicional e pilato, respectivamente. Em termos de produçao agricola, a eficiencia do sistema agropastoril, avaliado como produçao de grao de milho por area plantada, e 140% superior ao sistema pilato e 570% ao sistema tradicional. No sistema agropastoril, a ciclagem de carbono e nutrientes representa, juntamente com a adubaçao verde, as principais contribuiçoes para o uso eficiente dos nutrientes e para a sustentabilidade da produçao de milho
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