Engineering and Applications of fungal laccases for organic synthesis

Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols and anilines by one-electron abstraction, with the concomitant reduction of oxygen to water in a four-electron transfer process. In the presence of small redox mediators, laccase offers a broader repertory of oxidations including non-phenolic substrates. Hence, fungal laccases are considered as ideal green catalysts of great biotechnological impact due to their few requirements (they only require air, and they produce water as the only by-product) and their broad substrate specificity, including direct bioelectrocatalysis

The opengame competencies framework: an attempt to Map open education attitudes, knowledge and skills

Promoting Open Education Through Gamification - OpenGame, Funding entity European Union Call Erasmus + KA2 – Cooperation and Innovation for Good Practices. Strategic Partnerships for higher education - Reference 2019-1-ES01-KA203-0658152019-1-ES01-KA203-065815The paper introduces the competence framework produced by the OpenGame project, that includes the attitudes, knowledge and skills that educators need to master in order to work with Open Educational Practices (OEP). With this outcome, the OpenGame research team aims at closing the gap between the expanded interest of researchers and practitioners towards a holistic vision of open education and the absence of a sharedcompetence framework that can cover both the creation and/or use of Open Educational resources (OER) and the broader realm of OEP. Starting from literature review complemented with the analysis of 24 open teaching practices, 8 competences have been defined, related to both OER and open pedagogies. The competences relating to OER are: use open licences; search for OER; create, revise, and remix OER; and share OER. The competences relating to open pedagogy are: design open educational experiences; guide students to learn in the open; teach with OER; and implement open assessment. The framework details the knowledge and skills that correspond to each competence and can serve both as a starting point to build educators’ capacities to work with open approaches and as a reflexion tool to better understand what it means to be an Open Educator in the 21stcentury.info:eu-repo/semantics/publishedVersio

Modelos de gestión en nutrición clínica. Puntos débiles y puntos fuertes

En el VIº Foro de Debate Abbott-SENPE se establece una discusión multidisciplinar y multiprofesional para buscar el o los modelos de gestión clínica que consideramos más adecuados para la Unidades de Nutrición Clínica y Dietética (UNCyD) en España. Se valoran los puntos débiles y fuertes así como las oportunidades de los actuales sistemas y se concluye en la observación de una cierta disparidad vinculada no solo a las comunidades autónomas sino también a los diferentes tipos de hospital. Se propone, desde SENPE, la creación de un grupo de trabajo que ayude a la normalización de los modelos y a potenciar la cultura del Cuadro de Mandos Integral y de Gestión del Cambio.At the 6th Abbott-SENPE Debate Forum a multidisciplinary and multiprofessional discussion was established in order to seek for the model or the models of clinical management most appropriate for Clinical Nutrition and Dietetics Units (CNAD) in Spain. The weaknesses and strengths as well as opportunities for the current systems were assessed concluding that a certain degree of disparity was observed not only due to regional differences but also to different hospital types. It was proposed, from SENPE, the creation of a working group helping to standardize the models and promote the culture of Integral Control and Change Management

Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients

Background: Chagas disease reactivation is an AIDS defined illness that usually affects Central Nervous System. Gold-Standard diagnosis for T.cruzi reactivation is based on microscopical observation methods.Methods & Materials: Seven patients with HIV/AIDS diagnosis, T.cruzi serological findings, neurological disorders, and suspected of Chagas disease reactivation were included between 2015?2017 from two health centers of Buenos Aires, Argentina. Real-time PCRs (qPCR) against T.cruzi satellite DNA were carried out from cerebrospinal fluid (CSF) and peripheral blood samples (BS) for parasite load quantification. Molecular parasite characterization was based on amplification of spliced leader intergenic region, 24 srDNA, and A10 polymorphic sequences.Results: Patients were aged from 41 to 69 years old, 43% were women, CD4+ T cell counts were between 7 and 53 cell/mm3. All of them received tripanocidal treatment (TrypT).Five CSFs were withdrawn before TrypT starting. Two had microscopical detection of trypomastigote forms (MDTryp) and quantification over 500 p.e/mL by qPCR. The other 3 CSF samples were non-detectable by both methods. The remaining two patients CSF were obtained after starting TrypT with MDTryp negative findings but qPCR positive results with parasite burden below 13 p.e/mL.Among the 3 patients with negative CSF findings, 2 had positive MDTryp on BS with 119 and 3512 p.e/mL. The third was negative by MDTryp and had 2 p.e/mL before TrypT.All patients had qPCR positive findings on BS (2 -1426 p.e/mL) and decreased their parasitic loads during TrypT.All characterized parasites from BS and CSF samples belonged to DTU (Discrete Typing Unit) II, V or VI, frequently found in Southern Cone region.Out of this small series, 4 patients died: 2 because of brain ?Chagoma?, 1 due to status epilepticus and 1 due to acute abdomen. Two of the 3 survivors were those with negative CSF findings for both methods.Conclusion: Chagas disease reactivation occurred on deep inmunosupressed HIV people. Its diagnosis by MDTryp and qPCR is complementary. qPCR detected T.cruzi DNA on negative MDTryp findings even during treatment. TrypT always reduces T.cruzi DNA loads. qPCR might be an useful therapeutical marker.Fil: Fernandez, Marisa. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Besuschio, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Nicita, D.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Latini, V.. Sanatorio de la Trinidad Mitre; ArgentinaFil: Biondi, M. L.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: García, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Corti, Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Burgos, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina18th International Congress on Infectious Diseases (ICID)Ciudad Autónoma de Buenos AiresArgentinaInternational Society fot Infectious Disease

The first example of an intramolecular westphal reaction. Synthesis of a new aza-quinolizinium type system

The first example of an intramolecular Westphal condensation is described. To test the utility of this reaction, new benz(f)azino[2,1-a]phthalazinium salts have been prepared from appropriate dicarbonyl precursors.The authors wish to thank the Comisión Interministerial de Ciencia y Tecnología (C.I.C.Y.T.) (Project SAF98-0093) for financial support and one studentship (V. M. B.

Stars and brown dwarfs in the sigma Orionis cluster IV. IDS/INT and OSIRIS/GTC spectroscopy and Gaia DR2 astrometry

Context. Only a few open clusters are as important for the study of stellar and substellar objects, and their formation and evolution, as the young σ Orionis cluster. However, a complete spectroscopic characterisation of its whole stellar population is still missing. Aims. We filled most of that gap with a large spectroscopic and astrometric survey of targets towards σ Orionis. Eventually, it will be one of the open clusters with the lowest proportion of interlopers and the largest proportion of confirmed cluster members with known uncontrovertible youth features. Methods. We acquired 317 low-resolution optical spectra with the Intermediate Dispersion Spectrograph (IDS) at the 2.5 m Isaac Newton Telescope (INT) and the Optical System for Imaging and low Resolution Integrated Spectroscopy (OSIRIS) at the 10.4 m Gran Telescopio Canarias (GTC). We measured equivalent widths of Li i, Hα, and other key lines from these spectra, and determined spectral types. We complemented this information with Gaia DR2 astrometric data and other features of youth (mid-infrared excess, X-ray emission) compiled with Virtual Observatory tools and from the literature. Results. Of the 168 observed targets, we determined for the first time spectral types of 39 stars and equivalent widths of Li i and Hα of 34 and 12 stars, respectively. We identified 11 close (ρ </≈ 3 arcsec) binaries resolved by Gaia, of which three are new, 14 strong accretors, of which four are new and another four have Hα emission shifted by over 120 km s^(−1) , two juvenile star candidates in the sparse population of the Ori OB1b association, and one spectroscopic binary candidate. Remarkably, we found 51 non-clustermembers, 35 of which were previously considered as σ Orionis members and taken into account in high-impact works on, for example, disc frequency and initial mass function

Lifestyle and fat distribution in asthmatic and healthy adolescents

Introducción: el objetivo es analizar el estilo de vida, en función del ejercicio y la dieta, de un grupo de adolescentes de la Comunidad de Madrid, teniendo en cuenta género y patología y su relación con el estado nutricional, la distribución de grasa y la función pulmonar. Metodología: Estudio descriptivo de 207 sujetos que analizó el estilo de vida a partir del nivel de actividad física (AF) y la adherencia a la dieta mediterránea, el estado nutricional y la distribución de grasa, y la salud respiratoria. Resultados: El grupo de no asmáticos fue más activo (p=0,003) y presentó menor ICT (p=0,001) que el grupo de asmáticos. Se encontraron diferencias significativas dentro del grupo sin asma en nivel de AF siendo los varones más activos (p=0,01) y presentando menor índice cintura-talla (ICT) que las mujeres del mismo grupo (p=0,001). Conclusiones: Los adolescentes no asmáticos fueron más activos y presentaron mejor distribución de grasa que los asmáticosObjectives: objective is to analyze the lifestyle, integrating exercise and diet, of a group of adolescents from the Community of Madrid, taking into account gender and pathology and its relationship with nutritional status, fat distribution and lung function in adolescents with or without asthma. Methods: This was a descriptive study including 207 subjects aged 13.20 ± 0.62 years. Lifestyle was assessed in terms of physical activity (PA) levels, Mediterranean diet, nutritional status, and respiratory health measured through FEV1 (z). Results: In the non-asthma group, boys were more active (p = 0.01) and showed a lower waist-to-height ratio (WHtR) than girls (p = 0.001). Participants without asthma were more active (p = 0.003), and had a better WHtR (p = 0.001) and FEV1 (p = 0.001) than those with asthma. Conclusions: In this Spanish population sample, non-asthmatic adolescents were more active and showed a better nutritional status, fat distribution and respiratory health than their peers with asthmaFinanciado a través de la VII Convocatoria Real Madrid-Universidad Europea (Ref 2015/03RM); Tercer premio en los XV Premios Neumomadrid; Beca Jóvenes Investigadores de la SENP 2015