Binding of retinoids to uteroglobin

AbstractUteroglobin, a progesterone-binding secretory protein, was shown to bind retinoic acid and retinol in a non-saturable manner, at least up to concentrations of retinoids of 20 μM. Binding is increased about 10-fold by previous reduction of uteroglobin with 10 mM dithothreitol and it is not affected by previous saturation of the progesterone binding site, suggesting different binding sites for the steroid and the retinoids. The results are discussed in relation to a possible physiological role for this protein

Myristic acid potentiates palmitic acid-induced lipotoxicity and steatohepatitis associated with lipodystrophy by sustaning de novo ceramide synthesis.

Palmitic acid (PA) induces hepatocyte apoptosis and fuels de novo ceramide synthesis in the endoplasmic reticulum (ER). Myristic acid (MA), a free fatty acid highly abundant in copra/palmist oils, is a predictor of nonalcoholic steatohepatitis (NASH) and stimulates ceramide synthesis. Here we investigated the synergism between MA and PA in ceramide synthesis, ER stress, lipotoxicity and NASH. Unlike PA, MA is not lipotoxic but potentiated PA-mediated lipoapoptosis, ER stress, caspase-3 activation and cytochrome c release in primary mouse hepatocytes (PMH). Moreover, MA kinetically sustained PA-induced total ceramide content by stimulating dehydroceramide desaturase and switched the ceramide profile from decreased to increased ceramide 14:0/ceramide16:0, without changing medium and long-chain ceramide species. PMH were more sensitive to equimolar ceramide14:0/ceramide16:0 exposure, which mimics the outcome of PA plus MA treatment on ceramide homeostasis, than to either ceramide alone. Treatment with myriocin to inhibit ceramide synthesis and tauroursodeoxycholic acid to prevent ER stress ameliorated PA plus MA induced apoptosis, similar to the protection afforded by the antioxidant BHA, the pan-caspase inhibitor z-VAD-Fmk and JNK inhibition. Moreover, ruthenium red protected PMH against PA and MA-induced cell death. Recapitulating in vitro findings, mice fed a diet enriched in PA plus MA exhibited lipodystrophy, hepatosplenomegaly, increased liver ceramide content and cholesterol levels, ER stress, liver damage, inflammation and fibrosis compared to mice fed diets enriched in PA or MA alone. The deleterious effects of PA plus MA-enriched diet were largely prevented by in vivo myriocin treatment. These findings indicate a causal link between ceramide synthesis and ER stress in lipotoxicity, and imply that the consumption of diets enriched in MA and PA can cause NASH associated with lipodystrophy

Influence of anthropometry and body composition in climbing ability

INTRODUCTION: Climbing has become a popular, competitive and recreational sport. It requires, principally, skilled technique and high muscle strength. Therefore, it has been necessary to implement formal studies about the training of this discipline. Investigations have been performed some studies to demonstrate that the improvement of climbing can be explained with anthropometric and physiological variables. PURPOSE: The purpose of this study was evaluate the main variables that affect the performance of the climbing, namely, anthropometry, body composition and climbing level. METHODS: Eighteen climbers, ranged 22 - 40 years old and V2-V7 of climbing ability (according Hueco Tanks scale), were volunteered to participate. Measurements of anthropometric characteristics and body composition including height, arm spam, forearm maximum, midstylion-dactilion, lean arm mass, body mass index (BMI), skeletal muscle mass, body fat mass, visceral fat, were performed. All variables were evaluated by using a combination of a principal component analysis (PCA) with a cluster analysis. RESULTS: When the analysis was performed in both anthropometry (skeletal muscle mass, lean arm mass and forearm maximum) and body composition (BMI, body fat mass and visceral fat) groups of variables, the results showed an 86% of the variance. The anthropometry variables showed correlation with 60% of the total variance. Concretely, the higher climbing level athletes (V5-V7) showed a higher lean body mass and arm muscle mass than the lower climbing levels athletes (V0-V4). On the other hand, the last group showed higher body fat mass and visceral fat mass with respect to the V5-V7 climbing athletes. CONCLUSION: The data of the present study showed a clear tendency on the variables, both body composition and anthropometry, in terms of the climbing level. However, these results are not completely conclusive. We suggest necessary to improve the performance of the measurements in future studies

Spatial variation of the vegetative roughness in Mediterranean torrential streams affected by check dams

This is an Accepted Manuscript of an article published in Hydrological Sciences Journal on 2018, available online: http://www.tandfonline.com/10.1080/02626667.2017.1414384[EN] This study focuses on the spatial variations in vegetative roughness associated with morphological channel adjustments due to the presence of check dams in Mediterranean torrential streams. Manning's n values were estimated using methods established by previous studies of submerged and non-submerged vegetation in laboratory flume experiments and field work. The results showed a linear decrease in shrub density and rate of variation of the roughness coefficient versus degree of submergence with increasing distance upstream from the check dam, while downstream, the filling of the check dam and the bed incision had the most influence. A regression analysis was applied by fitting the data to different models: relationships between Manning's n and the flow velocity were found to be of the power type for shrubs in all upstream sections, while relationships of flow velocity versus hydraulic radius in the sections closest to check dams showed a good fit to second-order polynomial equations.This work was supported by project PI/13 (Hydrological and geomorphological response to the fluvial torrential systems affected by forestry-hydrological restoration works in semiarid catchments in southeast of Spain), from the Euromediterranean Institute of Hydrotechnics, European Council, and the Autonomous Community of Murcia, Spain.Conesa-García, C.; Sánchez-Tudela, JL.; Pérez-Cutillas, P.; Martinez-Capel, F. (2018). Spatial variation of the vegetative roughness in Mediterranean torrential streams affected by check dams. Hydrological Sciences Journal. 63(1):114-135. doi:10.1080/02626667.2017.1414384S11413563

The shape of our gut: Dissecting its impact on drug absorption in a 3D bioprinted intestinal model

The small intestine is a complex organ with a characteristic architecture and a major site for drug and nutrient absorption. The three-dimensional (3D) topography organized in finger-like protrusions called villi increases surface area remarkably, granting a more efficient absorption process. The intestinal mucosa, where this process occurs, is a multilayered and multicell-type tissue barrier. In vitro intestinal models are routinely used to study different physiological and pathological processes in the gut, including compound absorption. Still, standard models are typically two-dimensional (2D) and represent only the epithelial barrier, lacking the cues offered by the 3D architecture and the stromal components present in vivo, often leading to inaccurate results. In this work, we studied the impact of the 3D architecture of the gut on drug transport using a bioprinted 3D model of the intestinal mucosa containing both the epithelial and the stromal compartments. Human intestinal fibroblasts were embedded in a previously optimized hydrogel bioink, and enterocytes and goblet cells were seeded on top to mimic the intestinal mucosa. The embedded fibroblasts thrived inside the hydrogel, remodeling the surrounding extracellular matrix. The epithelial cells fully covered the hydrogel scaffolds and formed a uniform cell layer with barrier properties close to in vivo. In particular, the villus-like model revealed overall increased permeability compared to a flat counterpart composed by the same hydrogel and cells. In addition, the efflux activity of the P-glycoprotein (P-gp) transporter was significantly reduced in the villus-like scaffold compared to a flat model, and the genetic expression of other drugs transporters was, in general, more relevant in the villus-like model. Globally, this study corroborates that the presence of the 3D architecture promotes a more physiological differentiation of the epithelial barrier, providing more accurate data on drug absorbance measurements.Copyright © 2023. Published by Elsevier B.V

Tuning the size, composition and structure of Au and Co50Au50 Nanoparticles by High-Power Impulse Magnetron Sputtering in gas-phase Synthesis

Gas-phase synthesis of nanoparticles with different structural and chemical distribution is reported using a circular magnetron sputtering in an ion cluster source by applying high-power impulses. The influence of the pulse characteristics on the final deposit was evaluated on Au nanoparticles. The results have been compared with the more common direct current approach. In addition, it is shown for the first time that high-power impulses in magnetron based gas aggregation sources allows the growth of binary nanoparticles, CoAu in this case, with a variety of crystalline and chemical arrangements which are analyzed at the atomic level

Medición de la conductividad hidráulica bajo trayectorias horizontales en suelos granulares

In geotechnical structures, the permeability-dependent stability analysis is generally evaluated under vertical trajectories, because most permeameters are configured so that the water passes through the porous medium in this way. However, it is clear from the physical point of view that water can flow along different paths, including preferential ways that can include horizontal trajectories, parallel to the deposit of the stratum. The foregoing implies that both the vertical and horizontal component of the hydraulic conductivity or permeability coefficient must be estimated for a given stratum. The current research aims to explore possibilities for measuring the coefficient of permeability in horizontal trajectories, on granular soils, under a constant condition of relative density. For this purpose, a special chamber attached to a constant head permeameter was designed and constructed, which allows to measure the permeability in conditions of horizontal flow parallel to the soil layers. The proposed camera also admits the estimation of the permeability coefficient by combining stratifications of different granular soils, where the trajectories are not perfectly horizontal, but have diagonal paths. The results are compared with data obtained by conventional vertical flow permeameters, in order to check the difference in the measurements considering both situations in the samples. As a conclusion, it is important to report that there is evidently a difference in the permeability coefficients measured under different trajectories,En las estructuras geotécnicas generalmente el análisis de estabilidad dependiente de la permeabilidad, es evaluado bajo trayectorias verticales, debido a que la mayoría de permeámetros están configurados para que el agua atraviese de esta manera el medio poroso. No obstante, es claro desde el punto de vista físico que el agua puede fluir siguiendo diferentes caminos, entre ellos recorridos preferenciales que pueden incluir trayectorias horizontales paralelas a la depositación del estrato. Lo anterior implica que se debe estimar para un estrato, tanto la componente vertical, como horizontal de la conductividad hidráulica o coeficiente de permeabilidad. En la investigación actual se pretende explorar posibilidades de medición del coeficiente de permeabilidad en trayectorias horizontales, en suelos granulares, bajo una condición constante de densidad relativa. Para ello se diseñó y construyó una cámara especial adosada a un permeámetro de cabeza constante, que permite medir la permeabilidad en condiciones de flujo horizontal paralelo a los estratos. La cámara propuesta admite también, la estimación del coeficiente de permeabilidad combinando estratificaciones de diferentes suelos granulares, donde las trayectorias no son perfectamente horizontales, sino presentan recorridos diagonales. Los resultados son comparados con datos obtenidos mediante permeámetros convencionales de flujo vertical, con el fin de comprobar la diferencie en las mediciones considerando ambas situaciones en las muestras. Como conclusión generar es importante reportar que evidentemente existe una diferencia en los coeficientes de permeabilidad medidos bajo diferentes trayectorias