Análisis de antígenos presentes en célulasembrionarias humanas

En la tesis doctoral que presento se describen una serie de anticuerpos y las moléculas que estos reconocen. Trata de un tipo específico de anticuerpos, los anticuerpos monoclonales murinos, y de las técnicas inmunológicas que he empleado para su caracterización. Trata de las moléculas (proteínas) que se han detectado en estos tres años de trabajo tras la inmunización de ratones con extractos de células embrionarias humanas como inmunógeno, y de las diversas técnicas que han permitido, en tres casos, determinar sus características, la tesis, por ello, se ha dividido en capítulos que presentan a las diferentes moléculas. Trata, también, del posible empleo que estos anticuerpos puedan tener en el diagnostico de enfermedades y, de cómo se pueden hacer visibles, como herramientas útiles, para el trabajo en laboratorios de biomedicina de universidades, centros de investigación y hospitales, siendo como lo son, la última tecnología, de libre uso y no sujeto, por tanto, a ninguna patente que impida su aplicación sin restricciones

Future voyages to the solar system

CHALLENGE 2: FUTURE VOYAGES TO THE SOLAR SYSTEMPeer reviewe

A crowdsourcing database for the copy-number variation of the spanish population

Background: Despite being a very common type of genetic variation, the distribution of copy-number variations (CNVs) in the population is still poorly understood. The knowledge of the genetic variability, especially at the level of the local population, is a critical factor for distinguishing pathogenic from non-pathogenic variation in the discovery of new disease variants. Results: Here, we present the SPAnish Copy Number Alterations Collaborative Server (SPACNACS), which currently contains copy number variation profiles obtained from more than 400 genomes and exomes of unrelated Spanish individuals. By means of a collaborative crowdsourcing effort whole genome and whole exome sequencing data, produced by local genomic projects and for other purposes, is continuously collected. Once checked both, the Spanish ancestry and the lack of kinship with other individuals in the SPACNACS, the CNVs are inferred for these sequences and they are used to populate the database. A web interface allows querying the database with different filters that include ICD10 upper categories. This allows discarding samples from the disease under study and obtaining pseudo-control CNV profiles from the local population. We also show here additional studies on the local impact of CNVs in some phenotypes and on pharmacogenomic variants. SPACNACS can be accessed at: http://csvs.clinbioinfosspa.es/spacnacs/. Conclusion: SPACNACS facilitates disease gene discovery by providing detailed information of the local variability of the population and exemplifies how to reuse genomic data produced for other purposes to build a local reference database.This work is supported by Grants PID2020-117979RB-I00 from the Spanish Ministry of Science and Innovation; by the Institute of Health Carlos III (project IMPaCT-Data, exp. IMP/00019, IMP/00009 and PI20/01305), co-funded by the European Union, European Regional Development Fund (ERDF, “A way to make Europe”)

A crowdsourcing database for the copy-number variation of the Spanish population

Background: Despite being a very common type of genetic variation, the distribution of copy-number variations (CNVs) in the population is still poorly understood. The knowledge of the genetic variability, especially at the level of the local population, is a critical factor for distinguishing pathogenic from non-pathogenic variation in the discovery of new disease variants. Results: Here, we present the SPAnish Copy Number Alterations Collaborative Server (SPACNACS), which currently contains copy number variation profiles obtained from more than 400 genomes and exomes of unrelated Spanish individuals. By means of a collaborative crowdsourcing effort whole genome and whole exome sequencing data, produced by local genomic projects and for other purposes, is continuously collected. Once checked both, the Spanish ancestry and the lack of kinship with other individuals in the SPACNACS, the CNVs are inferred for these sequences and they are used to populate the database. A web interface allows querying the database with different filters that include ICD10 upper categories. This allows discarding samples from the disease under study and obtaining pseudo-control CNV profiles from the local population. We also show here additional studies on the local impact of CNVs in some phenotypes and on pharmacogenomic variants. SPACNACS can be accessed at: http://csvs.clinbioinfosspa.es/spacnacs/. Conclusion: SPACNACS facilitates disease gene discovery by providing detailed information of the local variability of the population and exemplifies how to reuse genomic data produced for other purposes to build a local reference database

PhDAY 2020 -FOO (Facultad de Óptica y Optometría)

Por cuarto año consecutivo los doctorandos de la Facultad de Óptica y Optometría de la Universidad Complutense de Madrid cuentan con un congreso propio organizado por y para ellos, el 4º PhDAY- FOO. Se trata de un congreso gratuito abierto en la que estos jóvenes científicos podrán presentar sus investigaciones al resto de sus compañeros predoctorales y a toda la comunidad universitaria que quiera disfrutar de este evento. Apunta en tu agenda: el 15 de octubre de 2020. En esta ocasión será un Congreso On-line para evitar que la incertidumbre asociada a la pandemia Covid-19 pudiera condicionar su celebración

Monoclonal Antibody That Recognizes a Domain on Heterogeneous Nuclear Ribonucleoprotein K and PTB-Associated Splicing Factor

hnRNP K protein is a member of the heterogeneous nuclear protein (hnRNP) complex that, besides its function as a translational regulator of human mRNA, is also considered to be a transcription factor involved in tumorigenesis. PSF is a protein part of the human spliceosome and essential in RNA splicing. Here we report the generation of one monoclonal antibody GG6H9.1C3 that recognized both hnRNP K and PSF proteins using Western blot analysis, flow cytometry, and immunocytochemistry.This work was funded by Fundación Progreso y Salud (FPS), Junta de Andalucía, Spain (D.L.); Instituto de Salud Carlos III (TERCEL RD06/0010/0025) (B.S.); and Fundación Progreso y Salud–Junta de Andalucía (PI-0095/2007) and Instituto de Salud Carlos III (CIBERDEM) ( J.R.T.). Also appreciated is funding from Consejería de Salud, Junta de Andalucía (EF-0005/2008) (G.G.).Peer Reviewe

Targeting neural stem cells with titanium dioxide nanoparticles coupled to specific monoclonal antibodies

22 páginas, 4 figuras -- PAGS nros. 1069-1089Aiming to characterize the use of biomaterials in cancer therapy, we took advantage of the n-type semiconductor properties, which upon irradiation excite their electrons into the conduction band to induce photoelectrochemical reactions generating oxygen reactive species (ROS). Indeed, photoactivated TiO(2) nanoparticles have been shown to kill in vitro either bacteria or tumor cells in culture following UV irradiation, as a consequence of the ROS levels generated; the killing was highly effective although devoid of specificity. In this report, we have directed the TiO(2) nanoparticles to particular targets by coupling them to the monoclonal antibody (mAb) Nilo1, recognizing a surface antigen in neural stem cells within a cell culture, to explore the possibility of making this process specific. TiO(2) nanoparticles generated with particular rutile/anatase ratios were coupled to Nilo1 antibody and the complexes formed were highly stable. The coupled antibody retained the ability to identify neural stem cells and upon UV irradiation, the TiO(2) nanoparticles were activated, inducing the selective photokilling of the antibody-targeted cells. Thus, these data indicate that antibody-TiO(2) complexes could be used to specifically remove target cell subpopulations, as demonstrated with neural stem cells. The possible applications in cancer therapy are discussedThe work in the author’s laboratories has been supported by grants: SAF2006-4826 from the Spanish Ministry of Science and Innovation; RD06/0010/1010 from the Spanish Ministry of Health; S-BIO-204/2006 from the Comunidad de Madrid; and PIF200620F0041 from the Spanish National Research Council (to A.S.), NEST/STREP (FP6) 028473-2 from European Union; and PIF-200660F0103 from CSIC (to JRJ)Peer reviewe

The sacralization process of space in Gadir/Gades. The archaeological site of Casa del Obispo (Cádiz)

A finales del siglo VI a. C., en el archipiélago gaditano se erigió un monumento funerario con claras evidencias del alto status social del individuo enterrado. El monumento se convirtió en el punto de partida de la sacralización de un lugar conocido popularmente como «Entre Catedrales», donde siempre ha prevalecido su carácter religioso, plasmado en diferentes manifestaciones arquitectónicas que se adaptan a las necesidades de culto y organizativas de la religión vigente en cada período histórico. Este artículo presenta las transformaciones cultuales de este lugar durante el período de incorporación de Gadir en la órbita romana, reflejo del peculiar proceso de romanización que sufrió la antigua ciudad gadirita. El nuevo elemento ritual elegido fue el agua, por lo que se levantó en el siglo II a. C. un complejo ricamente ornamentado, con una fuente, una pileta lustral y seis cisternas. El espacio elegido fue una zona abierta empleada durante el período de ocupación púnico para realizar diferentes tipos de rituales como banquetes, ofrendas aromáticas, etc.In the late 6th century BC, a funerary monument with clear evidence of the high social status of the individual buried in was erected in the Cadiz archipelago. The monument became the starting point of the sacralization of a place popularly known as «Entre Catedrales», whose religious nature has always prevailed, expressed in different architectural representations that adapt to the religious and organizational needs of the current religion in each historical period. This article presents the cultural transformations of this place during the period when Gadir was brought into the Roman orbit, a reflection of the peculiar Romanization process suffered by the ancient city of Gadir. The new ritual element chosen was water and, for this reason, a complex was built in the 2nd century BC with a fountain, a lustral font and six cisterns. The chosen space was an open area used during the Punic period to perform different types of rituals such as banquets, aromatic offerings, etc