4,455 research outputs found
Entropy of a Turbulent Bose-Einstein Condensate
Quantum turbulence deals with the phenomenon of turbulence in quantum fluids,
such as superfluid helium and trapped Bose-Einstein condensates (BECs).
Although much progress has been made in understanding quantum turbulence,
several fundamental questions remain to be answered. In this work, we
investigated the entropy of a trapped BEC in several regimes, including
equilibrium, small excitations, the onset of turbulence, and a turbulent state.
We considered the time evolution when the system is perturbed and let to evolve
after the external excitation is turned off. We derived an expression for the
entropy consistent with the accessible experimental data, that is, using the
assumption that the momentum distribution is well-known. We related the
excitation amplitude to different stages of the perturbed system, and we found
distinct features of the entropy in each of them. In particular, we observed a
sudden increase in the entropy following the establishment of a particle
cascade. We argue that entropy and related quantities can be used to
investigate and characterize quantum turbulence.Comment: 14 pages, 5 figure
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase ® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose ® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. © 2014 by the authors.We gratefully recognize the support from the Spanish Government, grant CTQ2009-07568 and
CTQ2013-41507-R and CNPq (Brazil). The predoctoral fellowships for García-Galán (Spanish
Government) and dos Santos (CNPq, Brazil) are also recognized. The authors wish to thank Ramiro
Martínez (Novozymes, Spain) for kindly supplying the enzymes used in this research.
The help and comments from Ángel Berenguer (Instituto de Materiales, Universidad de Alicante) are
kindly acknowledged. We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)Peer Reviewe
The Effect of Aluminium Surface Treatments on the Bonding Properties of Silica-Modified Epoxy Adhesive Joints: A Statistical Approach
A full factorial design is carried out to investigate the effects of different surface treatments, the inclusion of silica microparticles and the use of wash primer on the apparent shear strength and adherent strength of single-lap aluminium joints. Scanning electron microscopy, surface energy and roughness measurements are performed to characterise the aluminium surface. The results show that the use of wash primer decreases the apparent shear strength of the joints significantly. The cohesive failure of the primer is the main cause of the reduction in strength. On the other hand, the inclusion of 10 wt.% of silica microparticles in the adhesive layers increases the shear strength by 26%. Surfaces treated with NaOH for one minute, without using a wash primer, result in the most resistant joint. In contrast to the apparent shear strength, adherent strength is most effective when only degreasing is considered
A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria
Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope. PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG. This work describes the structural and functional characterization of an NlpC/P60-contain-ing peptidase from Photobacterium damselae subsp. piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry. PnpA (Photobacterium NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-D-glutamyl-meso-diaminopimelic acid bond. However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species. Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus. This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments. Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG. IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell. Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components. Here, it is shown that Photobacterium damselae subsp. piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients. The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.Europeu de Desenvolvimento Regional (FEDER) funds through the COMPETE 2020 Operacional Program for Competitiveness and Internationalization (POCI), Portugal 2020, and by Portuguese funds through Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior and Fundação para a Ciência e a Tecnologia (FCT), I.P., within the scope of the Norma Transitória - DL57/2016/CP1355/CT0010. This work had also support from the State Agency for Research (AEI) of Spain cofunded by the FEDER Program from the European Union (grants AGL2016-79738-R and
BIO2016-77639-P
Ground state and elementary excitations of single and binary Bose-Einstein condensates of trapped dipolar gases
We analyze the ground-state properties and the excitation spectrum of
Bose-Einstein condensates of trapped dipolar particles. First, we consider the
case of a single-component polarized dipolar gas. For this case we discuss the
influence of the trapping geometry on the stability of the condensate as well
as the effects of the dipole-dipole interaction on the excitation spectrum. We
discuss also the ground state and excitations of a gas composed of two
antiparallel dipolar components.Comment: 12 pages, 9 eps figures, final versio
Mitochondrial echoes of first settlement and genetic continuity in El Salvador
Background: From Paleo-Indian times to recent historical episodes, the Mesoamerican isthmus played an important role in the distribution and patterns of variability all around the double American continent. However, the amount of genetic information currently available on Central American continental populations is very scarce. In order to shed light on the role of Mesoamerica in the peopling of the New World, the present study focuses on the analysis of the mtDNA variation in a population sample from El Salvador.
Methodology/Principal Findings: We have carried out DNA sequencing of the entire control region of the mitochondrial DNA (mtDNA) genome in 90 individuals from El Salvador. We have also compiled more than 3,985 control region profiles from the public domain and the literature in order to carry out inter-population comparisons. The results reveal a predominant Native American component in this region: by far, the most prevalent mtDNA haplogroup in this country (at ~90%) is A2, in contrast with other North, Meso- and South American populations. Haplogroup A2 shows a star-like phylogeny and is very diverse with a substantial proportion of mtDNAs (45%; sequence range 16090–16365) still unobserved in other American populations. Two different Bayesian approaches used to estimate admixture proportions in El Salvador shows that the majority of the mtDNAs observed come from North America. A preliminary founder analysis indicates that the settlement of El Salvador occurred about 13,400±5,200 Y.B.P.. The founder age of A2 in El Salvador is close to the overall age of A2 in America, which suggests that the colonization of this region occurred within a few thousand years of the initial expansion into the Americas.
Conclusions/Significance: As a whole, the results are compatible with the hypothesis that today's A2 variability in El Salvador represents to a large extent the indigenous component of the region. Concordant with this hypothesis is also the observation of a very limited contribution from European and African women (~5%). This implies that the Atlantic slave trade had a very small demographic impact in El Salvador in contrast to its transformation of the gene pool in neighbouring populations from the Caribbean facade
The Hubble PanCET program: The near-ultraviolet transmission spectrum of WASP-79b
We present Hubble Space Telescope (HST) transit observations of the
Hot-Jupiter WASP-79b acquired with the Space Telescope Imaging Spectrograph
(STIS) in the near ultraviolet (NUV). Two transit observations, part of the
PanCET program, are used to obtain the transmission spectra of the planet
between 2280 and 3070{\AA}. We correct for systematic effects in the raw data
using the jitter engineering parameters and polynomial modelling to fit the
white light curves of the two transits. We observe an increase in the
planet-to-star radius ratio at short wavelengths, but no spectrally resolved
absorption lines. The difference between the radius ratios at 2400 and
3000{\AA} reaches (4.5). Although the NUV
transmission spectrum does not show evidence of hydrodynamical escape, the
strong atmospheric features are likely due to species at very high altitudes.
We performed a 1D simulation of the temperature and composition of WASP-79b
using Exo-REM. The temperature pressure profile crosses condensation curves of
radiatively active clouds, particularly MnS, MgSiO, Fe, and
AlO. Still, none of these species produces the level of observed
absorption at short wavelengths and can explain the observed increase in the
planet's radius. WASP-79b's transit depth reaches 23 scale height, making it
one of the largest spectral features observed in an exoplanet at this
temperature (1700 K). The comparison of WASP-79b's transmission spectrum
with three warmer hot Jupiters shows a similar level of absorption to WASP-178b
and WASP-121b between 0.2 and 0.3m, while HAT-P-41b's spectrum is flat.
The features could be explained by SiO absorption.Comment: Accepted for publication January 31, 2023 in the Journal Astronomy &
Astrophysic
Caracterización de la actividad enzimática del jugo gástrico de pulpo, Octopus vulgaris y de choco, Sepia officinalis a distintos pH. Digestibilidad in vitro de distintas dietas, con jugo gástrico de pulpo
Intensive culture of marine species has been increasing in many countries, such
as Japan, Norway, Spain, France, and Greece. Recent research to improve the quality and
quantity of aquatic animals production by understanding the process of feeding (ingestion,
digestion, and absorption), with emphasis on the digestive enzymatic capabilities is important
in order to understand metabolic processes and reduce costs and time on the development
of such diets. The impetus to increase knowledge about suitable inert diets to partially or
totally replace live feed would reduce this expensive part of the operation, particularly in new
potential species, such as the octopus, Octopus vulgaris, and the cuttlefi sh, Sepia offi cinalis,
which are two of the most promising cephalopods for large-scale culture. This work is
divided into two parts: (1) Enzymatic characterization, by determination of protease activity
of the gastric juice at pH ranges from 2 to 12, since this is the major responsible of digestion
processes, and (2) In vitro evaluation of several prepared diets, as well as a selection of
several natural animal protein sources, by means of a pH-Stat system The results of Part 1 showed that the digestive enzyme activity in total proteases from the gastric juice was higher
at pH 7 and 9, therefore being a more alkaline digestion for these species. Total proteases
activity was higher at pH 8, while the tripsin activity was higher at pH 7, for both species.
For Part 2, the natural diets (natural or liophylized squid, blue whiting and mussel) promoted
the highest digestibility, with the highest value being attained with liophylized squid (49%).
From the prepared diets, based on raw blue whiting paste, those diets agglutinated with
gelatine delivered the highest digestibility. The use of soy protein, as well as heating gelatine
as agglutinants, promoted poor digestibility
Understanding the antimicrobial mechanism of TiO2-based nanocomposite films in a pathogenic bacterium
Titania (TiO2)-based nanocomposites subjected to light excitation are remarkably effective in eliciting microbial death. However, the mechanism by which these materials induce microbial death and the effects that they have on microbes are poorly understood. Here, we assess the low dose radical-mediated TiO2 photocatalytic action of such nanocomposites and evaluate the genome/proteome-wide expression profiles of Pseudomonas aeruginosa PAO1 cells after two minutes of intervention. The results indicate that the impact on the gene-wide flux distribution and metabolism is moderate in the analysed time span. Rather, the photocatalytic action triggers the decreased expression of a large array of genes/proteins specific for regulatory, signalling and growth functions in parallel with subsequent selective effects on ion homeostasis, coenzyme-independent respiration and cell wall structure. The present work provides the first solid foundation for the biocidal action of titania and may have an impact on the design of highly active photobiocidal nanomaterial
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Demonstration of the event identification capabilities of the NEXT-White detector
In experiments searching for neutrinoless double-beta decay, the possibility of identifying the two emitted electrons is a powerful tool in rejecting background events and therefore improving the overall sensitivity of the experiment. In this paper we present the first measurement of the efficiency of a cut based on the different event signatures of double and single electron tracks, using the data of the NEXT-White detector, the first detector of the NEXT experiment operating underground. Using a 228Th calibration source to produce signal-like and background-like events with energies near 1.6 MeV, a signal efficiency of 71.6 ± 1.5 stat± 0.3 sys% for a background acceptance of 20.6 ± 0.4 stat± 0.3 sys% is found, in good agreement with Monte Carlo simulations. An extrapolation to the energy region of the neutrinoless double beta decay by means of Monte Carlo simulations is also carried out, and the results obtained show an improvement in background rejection over those obtained at lower energies. [Figure not available: see fulltext.
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