SYNTHESIS OF FLUORINE-CONTAINING N,O-HETEROCYCLES ON THE BASE OF 4,5-BIS(TRIFLUOROMETHYL)IMIDAZOLIDINE-2-ONES, 2-AMINOETHANOL AND 2-AMINOPHENOL

This work was carried out within the framework of State task AAAA-A19-119011790132-7

Kinetic Resolution of Racemic 2-Aryloxy Propionyl Chlorides Using Enantiopure (S)-3,4-Dihydro-3-methyl-2H-[1,4]benzoxazines

Stereoselectivity in the mutual kinetic resolution (KR) of racemic 3,4-dihydro-3-methyl-2H-[1,4]benzoxazines and racemic 2-aryloxy propionyl chlorides was studied. Based on the results obtained, preparative methods for single enantiomers of a series of 2-aryloxy propionic acids via acylative KR of their racemates with enantiopure (S)-3,4-dihydro-3-methyl-2H-[1,4]benzoxazines have been proposed. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, WeinheimThe work was financially supported by the Russian Science Foundation (grant 19‐13‐00231, in the part of studying mutual kinetic resolution) and the Russian Foundation for Basic Research (grant 18‐33‐00027_mol_a, in the part of preparation of enantiomers of 2‐aryloxy propionic acids). Analytical studies were carried out using equipment of the Centre for Joint Use “Spectroscopy and Analysis of Organic Compounds” at the Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch

Hemozoin "knobs" in Opisthorchis felineus infected liver

Background Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin “knobs” – the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed

Hemozoin "knobs" in Opisthorchis felineus infected liver

Background Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin “knobs” – the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed