Nutritional Manipulation of One-Carbon Metabolism: Effects on Arsenic Methylation and Toxicity

Exposure to arsenic (As) through drinking water is a substantial problem worldwide. The methylation of As, a reactive metalloid, generates monomethyl- (MMA) and dimethyl-arsenical (DMA) species. The biochemical pathway that catalyzes these reactions, one-carbon metabolism, is regulated by folate and other micronutrients. Arsenic methylation exerts a critical influence on both its urinary elimination and chemical reactivity. Mice having the As methyltransferase null genotype show reduced urinary As excretion, increased As retention, and severe systemic toxicity. The most toxic As metabolite in vitro is MMAIII, an intermediate in the generation of DMAV, a much less toxic metabolite. These findings have raised the question of whether As methylation is a detoxification or bioactivation pathway. Results of population-based studies suggest that complete methylation of inorganic As to DMA is associated with reduced risk for As-induced health outcomes, and that nutrients involved in one-carbon metabolism, such as folate, can facilitate As methylation and elimination

XX/XY Sex Chromosomes in the South American Dwarf Gecko (\u3cem\u3eGonatodes humeralis\u3c/em\u3e)

Sex-specific genetic markers identified using restriction site-associated DNA sequencing, or RADseq, permits the recognition of a species’ sex chromosome system in cases where standard cytogenetic methods fail. Thus, species with male-specific RAD markers have an XX/XY sex chromosome system (male heterogamety) while species with female-specific RAD markers have a ZZ/ZW sex chromosome (female heterogamety). Here, we use RADseq data from 5 male and 5 female South American dwarf geckos (Gonatodes humeralis) to identify an XX/XY sex chromosome system. This is the first confidently known sex chromosome system in a Gonatodes species. We used a low-coverage de novo G. humeralis genome assembly to design PCR primers to validate the male-specificity of a subset of the sex-specific RADseq markers and describe how even modest genome assemblies can facilitate the design of sex-specific PCR primers in species with diverse sex chromosome systems

Transcriptomic data support a nocturnal bottleneck in the ancestor of gecko lizards

Gecko lizards are a species-rich clade of primarily-nocturnal squamate reptiles. In geckos, adaptations to nocturnality have dramatically reshaped the eye. Perhaps the most notable change is the loss of rod cells in the retina and subsequent “transmutation” of cones into a rod-like morphology and physiology. While many studies have noted the absence of some rod-specific genes, such as the visual pigment Rhodopsin (RH1), these studies have focused on just a handful of species that are nested deep in the gecko phylogeny. Thus, it is not clear whether these changes arose through convergence, are homologous and ubiquitous across geckos, or restricted to a subset of species. Here, we used de novo eye transcriptomes from five gecko species, and genomes from two additional gecko species, representing the breadth of extant gecko diversity (i.e. 4 of the 7 gecko families, spanning the deepest divergence of crown Gekkota), to show that geckos lost expression of almost the entire suite of necessary rod-cell phototransduction genes in the eye, distinct from all other squamate reptiles. Geckos are the first vertebrate group to have lost their complete rod-cell expression pathway, not just the visual pigment. In addition, all sampled species have also lost expression of the cone-opsin SWS2 visual pigment. These results strongly suggest a single loss of rod cells and subsequent cone-to-rod transmutation that occurred prior to the diversification of extant geckos

Abstracting PROV provenance graphs:A validity-preserving approach

Data provenance is a structured form of metadata designed to record the activities and datasets involved in data production, as well as their dependency relationships. The PROV data model, released by the W3C in 2013, defines a schema and constraints that together provide a structural and semantic foundation for provenance. This enables the interoperable exchange of provenance between data producers and consumers. When the provenance content is sensitive and subject to disclosure restrictions, however, a way of hiding parts of the provenance in a principled way before communicating it to certain parties is required. In this paper we present a provenance abstraction operator that achieves this goal. It maps a graphical representation of a PROV document PG1 to a new abstract version PG2, ensuring that (i) PG2 is a valid PROV graph, and (ii) the dependencies that appear in PG2 are justified by those that appear in PG1. These two properties ensure that further abstraction of abstract PROV graphs is possible. A guiding principle of the work is that of minimum damage: the resultant graph is altered as little as possible, while ensuring that the two properties are maintained. The operator developed is implemented as part of a user tool, described in a separate paper, that lets owners of sensitive provenance information control the abstraction by specifying an abstraction policy.</p

The Discovery of XY Sex Chromosomes in a \u3cem\u3eBoa\u3c/em\u3e and \u3cem\u3ePython\u3c/em\u3e

For over 50 years, biologists have accepted that all extant snakes share the same ZW sex chromosomes derived from a common ancestor [1, 2, 3], with different species exhibiting sex chromosomes at varying stages of differentiation. Accordingly, snakes have been a well-studied model for sex chromosome evolution in animals [1, 4]. A review of the literature, however, reveals no compelling support that boas and pythons possess ZW sex chromosomes [2, 5]. Furthermore, phylogenetic patterns of facultative parthenogenesis in snakes and a sex-linked color mutation in the ball python (Python regius) are best explained by boas and pythons possessing an XY sex chromosome system [6, 7]. Here we demonstrate that a boa (Boa imperator) and python (Python bivittatus) indeed possess XY sex chromosomes, based on the discovery of male-specific genetic markers in both species. We use these markers, along with transcriptomic and genomic data, to identify distinct sex chromosomes in boas and pythons, demonstrating that XY systems evolved independently in each lineage. This discovery highlights the dynamic evolution of vertebrate sex chromosomes and further enhances the value of snakes as a model for studying sex chromosome evolution

First Report of Sex Chromosomes in Night Lizards (Scincoidea: Xantusiidae)

Squamate reptiles (lizards, snakes, and amphibians) are an outstanding group for studying sex chromosome evolution—they are old, speciose, geographically widespread, and exhibit myriad sex-determining modes. Yet, the vast majority of squamate species lack heteromorphic sex chromosomes. Cataloging the sex chromosome systems of species lacking easily identifiable, heteromorphic sex chromosomes, therefore, is essential before we are to fully understand the evolution of vertebrate sex chromosomes. Here, we use restriction site-associated DNA sequencing (RADseq) to classify the sex chromosome system of the granite night lizard, Xantusia henshawi. RADseq is an effective alternative to traditional cytogenetic methods for determining a species’ sex chromosome system (i.e., XX/XY or ZZ/ZW), particularly in taxa with non-differentiated sex chromosomes. Although many xantusiid lineages have been karyotyped, none possess heteromorphic sex chromosomes. We identified a ZZ/ZW sex chromosome system in X. henshawi—the first such data for this family. Furthermore, we report that the X. henshawi sex chromosome contains fragments of genes found on Gallus gallus chromosomes 7, 12, and 18 (which are homologous to Anolis carolinensis chromosome 2), the first vertebrate sex chromosomes to utilize this linkage group

NDM-552: COMBINED PROBABILITIES OF PEAK WIND AND SNOW LOAD EVENTS

The National Building Code of Canada 2010 (NBCC) defines several loading combination scenarios for use in structural design. Appropriate combination factors are provided based on the probability of failure due to the simultaneous occurrence of the specified loads. Load Combination Cases 3 and 4 of Table 4.1.3.2.A include the combination of wind and snow loads, which are transient in nature. The recommended combination factors are intended to provide a uniform degree of reliability for design. However, in reality, the probability of the simultaneous loading due to wind and snow depends on the local meteorological climate. This probability can be more accurately simulated through the Finite Area Element (FAE) process, which studies the hour-by-hour accumulation and depletion of snow based on historical meteorological records. It takes into account variables such as wind speed and direction, temperature, humidity, water retention in a snow pack and many others. In the present work, the accumulation and depletion of snow on a modelled ground patch and the corresponding wind speeds were computed on an hourly basis to determine the correlation of wind and snow loads. Using this process, this paper investigates the interaction between wind and snow loads for 25 distinct regions in Canada, for both ground and roof snow loads

STR-940: PARAMETRIC SIMULATION OF ROOF STRUCTURAL SNOW LOADS

While the National Building Code of Canada (NBCC) provides engineers with suitable snow loading guidelines for structural design, the strict application of the code may not lead to an optimized structural design. Generalizations have been made to ensure the applicability of the code to the majority of potential structures within Canada, which result in conservative estimates in certain situations. In particular, the interaction between region-specific prevailing wind directionality, climate and roof orientation are not accounted for. However, the development of advanced physical and numerical snow simulation approaches allows for the investigation of building-specific variables that affect snow loading. The Finite Area Element (FAE) process simulates the hour-by-hour accumulation and depletion of snow on a specific building design. This tool provides detailed quantification of the probabilistic snow loading accounting for region-specific long term meteorological conditions and building-specific variables such as roof size, exposure to prevailing winds, thermal capacity and local aerodynamics. While providing a detailed assessment of the snow loads, a full FAE assessment can be both time consuming and relatively costly for many applications. This parametric analysis approach has been developed using a variety of simple building geometries to provide an approach to assess the relative impacts of many of the key variables needed to inform a design. This paper describes the physical and numerical models used for the parametric simulation of snow loads, and discusses their application to structures within Canada