440 research outputs found
Ultrasonic activation of irrigants increases growth factor release from human dentine.
OBJECTIVES
Bioactive proteins are sequestered in human dentine and play a decisive role in dental pulp regeneration and repair. They can be released and exposed on the dentine surface by acids, but also chelators, such as ethylenediaminetetraacetic acid (EDTA). The objectives of this study were (i) to evaluate whether ultrasonic activation of irrigants in the root canal will promote growth factor release from dentine and (ii) to collect bioactive proteins in a physiological solution.
MATERIALS AND METHODS
Human dentine disks underwent irrigation with and without ultrasonic activation. The protocols included treatment by either a single or two consecutive steps with 10 % EDTA and phosphate-buffered saline (PBS), where each sample was treated three times. To mimic clinical conditions, selected irrigation regimens were applied to root canals of extracted human teeth after preparation. Amounts of transforming growth factor β1 (TGF-β1) in solution were quantified using enzyme-linked immunosorbent assays. Nonparametric statistical analysis was performed to compare different groups as well as repetitions within a group (Mann-Whitney U test, α = 0.05). Additionally, morphological changes of dentine surfaces were visualized by scanning electron microscopy (SEM).
RESULTS
TGF-β1 was not detectable after irrigation of dentine with PBS, neither with nor without ultrasonic activation. Irrigation with EDTA released TGF-β1, and ultrasonic activation of EDTA enhanced this effect. However, preceding EDTA conditioning enabled the release of bioactive proteins into PBS solution. Similar results were observed in dentine disks and root canals. Visualization of dentine surfaces after different treatment revealed superficial erosion after ultrasonic activation irrespective of the irrigant solution, but different degrees of exposure of organic substance.
CONCLUSIONS
Ultrasonic activation enhances growth factor release from human dentine. Bioactive proteins can be isolated in physiological solvents and may act as autologous supplements for regenerative endodontic treatment or pulp tissue engineering.
CLINICAL RELEVANCE
Autologous growth factors from human dentine can advance treatment strategies in dental pulp tissue engineering
Endodontic management of traumatized permanent teeth : a comprehensive review
The pulp plays a key role in the treatment of traumatic dental injuries (TDIs) and is strongly associated with the outcome, particularly in severe cases. A correct pulp diagnosis is essential as it forms the basis for developing the appropriate management strategy. However, many TDIs are complex, and their treatment requires a profound knowledge of the physiological and pathological responses of the affected tissues. This comprehensive review will look at the dentine-pulp complex and its interaction with the surrounding tissues following TDIs. The literature up to 2020 was reviewed based on several searches on PubMed and the Cochrane Library using relevant terms. In addition to the recently revised guidelines of the International Association of Dental Traumatology, this article aims to provide background information with a focus on endodontic aspects and to gather evidence on which a clinician can make decisions on the choice of the appropriate endodontic approach for traumatized permanent teeth.Peer reviewe
European Society of Endodontology position statement : endodontic management of traumatized permanent teeth
This position statement represents a consensus of an expert committee convened by the European Society of Endodontology (ESE) on the endodontic management of traumatized permanent teeth. A recent comprehensive review with detailed background information provides the basis for this position statement (Krastl et al. 2021, International Endodontic Journal, ). The statement is based on current scienti?c evidence as well as the expertise of the committee. Complementing the recently revised guidelines of the International Association of Dental Traumatology, this position statement aims to provide clinical guidance for the choice of the appropriate endodontic approach for traumatized permanent teeth. Given the dynamic nature of research in this area, this position statement will be updated at appropriate intervals.Peer reviewe
European Society of Endodontology position statement : endodontic management of traumatized permanent teeth
This position statement represents a consensus of an expert committee convened by the European Society of Endodontology (ESE) on the endodontic management of traumatized permanent teeth. A recent comprehensive review with detailed background information provides the basis for this position statement (Krastl et al. 2021, International Endodontic Journal, ). The statement is based on current scienti?c evidence as well as the expertise of the committee. Complementing the recently revised guidelines of the International Association of Dental Traumatology, this position statement aims to provide clinical guidance for the choice of the appropriate endodontic approach for traumatized permanent teeth. Given the dynamic nature of research in this area, this position statement will be updated at appropriate intervals.Peer reviewe
Compton Scattering by the Proton using a Large-Acceptance Arrangement
Compton scattering by the proton has been measured using the tagged-photon
facility at MAMI (Mainz) and the large-acceptance arrangement LARA. The new
data are interpreted in terms of dispersion theory based on the SAID-SM99K
parameterization of photo-meson amplitudes. It is found that two-pion exchange
in the t-channel is needed for a description of the data in the second
resonance region. The data are well represented if this channel is modeled by a
single pole with mass parameter m(sigma)=600 MeV. The asymptotic part of the
spin dependent amplitude is found to be well represented by pi-0-exchange in
the t-channel. A backward spin-polarizability of
gamma(pi)=(-37.1+-0.6(stat+syst)+-3.0(model))x10^{-4}fm^4 has been determined
from data of the first resonance region below 455 MeV. This value is in a good
agreement with predictions of dispersion relations and chiral pertubation
theory. From a subset of data between 280 and 360 MeV the resonance
pion-photoproduction amplitudes were evaluated leading to a E2/M1 multipole
ratio of the p-to-Delta radiative transition of EMR(340
MeV)=(-1.7+-0.4(stat+syst)+-0.2(model))%. It was found that this number is
dependent on the parameterization of photo-meson amplitudes. With the MAID2K
parameterization an E2/M1 multipole ratio of EMR(340
MeV)=(-2.0+-0.4(stat+syst)+-0.2(model))% is obtained
CD4<sup>+</sup> and CD8a<sup>+</sup> PET imaging predicts response to novel PD-1 checkpoint inhibitor:studies of Sym021 in syngeneic mouse cancer models
Applying SMT Solvers to the Test Template Framework
The Test Template Framework (TTF) is a model-based testing method for the Z
notation. In the TTF, test cases are generated from test specifications, which
are predicates written in Z. In turn, the Z notation is based on first-order
logic with equality and Zermelo-Fraenkel set theory. In this way, a test case
is a witness satisfying a formula in that theory. Satisfiability Modulo Theory
(SMT) solvers are software tools that decide the satisfiability of arbitrary
formulas in a large number of built-in logical theories and their combination.
In this paper, we present the first results of applying two SMT solvers, Yices
and CVC3, as the engines to find test cases from TTF's test specifications. In
doing so, shallow embeddings of a significant portion of the Z notation into
the input languages of Yices and CVC3 are provided, given that they do not
directly support Zermelo-Fraenkel set theory as defined in Z. Finally, the
results of applying these embeddings to a number of test specifications of
eight cases studies are analysed.Comment: In Proceedings MBT 2012, arXiv:1202.582
Neutron polarizabilities investigated by quasi-free Compton scattering from the deuteron
Measuring Compton scattered photons and recoil neutrons in coincidence,
quasi-free Compton scattering by the neutron has been investigated at MAMI
(Mainz) at in an energy range from 200 to 400 MeV.
From the data a polarizability difference of in units of has been
determined. In combination with the polarizability sum deduced from photo absorption data, the neutron electric and
magnetic polarizabilities, and ,
are obtained
A fast Monte Carlo algorithm for site or bond percolation
We describe in detail a new and highly efficient algorithm for studying site
or bond percolation on any lattice. The algorithm can measure an observable
quantity in a percolation system for all values of the site or bond occupation
probability from zero to one in an amount of time which scales linearly with
the size of the system. We demonstrate our algorithm by using it to investigate
a number of issues in percolation theory, including the position of the
percolation transition for site percolation on the square lattice, the
stretched exponential behavior of spanning probabilities away from the critical
point, and the size of the giant component for site percolation on random
graphs.Comment: 17 pages, 13 figures. Corrections and some additional material in
this version. Accompanying material can be found on the web at
http://www.santafe.edu/~mark/percolation
Compton scattering on the nucleon at intermediate energies and polarizabilities in a microscopic model
A microscopic calculation of Compton scattering on the nucleon is presented
which encompasses the lowest energies -- yielding nucleon polarizabilities --
and extends to energies of the order of 600 MeV. We have used the covariant
"Dressed K-Matrix Model" obeying the symmetry properties which are appropriate
in the different energy regimes. In particular, crossing symmetry, gauge
invariance and unitarity are satisfied. The extent of violation of analyticity
(causality) is used as an expansion parameter.Comment: 35 pages, 15 figures, using REVTeX. Modified version to be published
in Phys. Rev. C, more extensive comparison with data for Compton scattering,
all results unchange
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