Duration of postpartum anestrus in ewes of the Mexican high plateau

Duration of the postpartum anestrus (DPA) was studied in a typical Mexican flock using 78 ewes, Dorset x Creole and Dorset x Suffolk crosses, that lambed from September to July during the first 10 days of each month. Samples of jugular vein blood (5mL) were taken during the first 90 days postpartum, for determination of progesterone concentration until this reached a level of 1 ng.mL-1 or greater, indicating the end of estrus. The other variables analyzed were phenotype of the ewe, type of parturition (single or twins), parturition number, sex of the offspring, and month of parturition. Data were subjected to analysis of variance using a model of all fixed effects without interaction. DAP was calculated as a function of the period of year in which lambing occurred, least squares means being obtained and compared by Tukey test. Large differences were found between months of parturition (P>.0001), and three periods of year were differentiated by relative DPA, i.e. short (25.90 ± 2.65 days, from June to November); medium (46.9 ± 2.14 days in December and May); long (70.5 ± 2.27 days from January to April). The other variables analyzed did not affect DPA (P < 0.10). These results suggest that DPA varies essentially with month of lambing and that it is possible to distinguish between the minimum physiological DPA and seasoned anestrus

Productive and reproductive performance of dual-purpose cows in two systems of suckling in the tropics

With the objective of finding an alternative management to improve the reproductive and productive efficiencyof dual-purpose Bos taurus x Bos indicus cows in a tropical environment, 20 adult, ¾ Brown Swiss x ¼ Cebu cows were assigned to two treatments: traditional suckling (AT; n = 10) and delayed suckling (AR; n = 10). In AT, three quarters were milked mechanically and one quarter was left for the calf; after milking, the calf remained with its mother for 7 h. Cows in AR were milked completely and a suckling period of 1 h took place 8 h after milking. Estrus was detected twice daily, with the help of a bull with a deviated penis. Milk production and liveweight of the calves and cows were recorded every 7 and 15 days, respectively. Statistical analysis utilized the t-test for samples of independent populations; a model of fixed effects without interaction, with suckling management and number of parturition as the principal effects; and Chi-square. The number of cows observed in estrus was greater (P<.05) in AR (90%) than in AT (70%). Use of AR reduced by 11 d the interval from parturition to first estrus (69.9 ± 24.7 vs. 58.4 ± 23.6). No differences (P>.05) were found in rate of weight gain of the calves (637 ± 200 vs. 724 ± 100 g/d), in milk obtained mechanically (4.4 ± 1.5 vs. 5.1 ± 1.3 kg/d), or in liveweight change of the dams (-0.221 ± 0.2 vs -0.115 ± 0.3 kg/d) between AT and AR, respectively. Total milk production in AR exceeded (P<.05) that in AT (11.3 ± 1.6 vs. 9.4 ± 2.0 kg/d). It is concluded that delaying suckling for 8 h after milking benefits the interval from parturition to first estrus, the proportion of cows ovulating and milk production during the first 100 d postpartum, without adversely affecting calf growth, liveweight of the cow, and milk extracted mechanically

P-P Total Cross Sections at VHE from Accelerator Data

Comparison of P-P total cross-sections estimations at very high energies - from accelerators and cosmic rays - shows a disagreement amounting to more than 10 %, a discrepancy which is beyond statistical errors. Here we use a phenomenological model based on the Multiple-Diffraction approach to successfully describe data at accelerator energies. The predictions of the model are compared with data On the basis of regression analysis we determine confident error bands, analyzing the sensitivity of our predictions to the employed data for extrapolation. : using data at 546 and 1.8 TeV, our extrapolations for p-p total cross-sections are only compatible with the Akeno cosmic ray data, predicting a slower rise with energy than other cosmic ray results and other extrapolation methods. We discuss our results within the context of constraints in the light of future accelerator and cosmic ray experimental results.Comment: 26 pages aqnd 11 figure

Interactions among mitochondrial proteins altered in glioblastoma

Mitochondrial dysfunction is putatively central to glioblastoma (GBM) pathophysiology but there has been no systematic analysis in GBM of the proteins which are integral to mitochondrial function. Alterations in proteins in mitochondrial enriched fractions from patients with GBM were defined with label-free liquid chromatography mass spectrometry. 256 mitochondrially-associated proteins were identified in mitochondrial enriched fractions and 117 of these mitochondrial proteins were markedly (fold-change ≥2) and significantly altered in GBM (p ≤ 0.05). Proteins associated with oxidative damage (including catalase, superoxide dismutase 2, peroxiredoxin 1 and peroxiredoxin 4) were increased in GBM. Protein–protein interaction analysis highlighted a reduction in multiple proteins coupled to energy metabolism (in particular respiratory chain proteins, including 23 complex-I proteins). Qualitative ultrastructural analysis in GBM with electron microscopy showed a notably higher prevalence of mitochondria with cristolysis in GBM. This study highlights the complex mitochondrial proteomic adjustments which occur in GBM pathophysiology

Resource assessment of the marine current developed in the Cozumel Channel

Renewable energy based systems are expected to contribute on the reduction of greenhouse gases and carbon emission, while satisfying global energy demands. In Mexico, the Cozumel Channel located in the Caribbean Sea has been identified as a potential energy source in the region. Preliminary studies have shown that the ocean current is characterized by almost uniform and unidirectional flow velocities of up to 2.0 m/s within its mid-section with water depths > 500 m. Nevertheless, a detailed resource assessment in shallow waters of the Cozumel Channel is required to address sites potentially suitable for the installation of marine energy converters. Field measurements were taken during September 23rd-29th, 2018 to describe the spatial variation of the marine current velocities at various points along the east-side of the Cozumel Channel, at water depths less than 50 m. Flow velocities higher than 1.0 m/s were identified on the northern east of the Cozumel Channel, at a distance >600 m from the shoreline and over the continental shelf with water depths <50 m. Both energy and power intensity exceedance curves were developed from depth averaged velocities from ADCP measurements. Potential sites were identified where an array of marine energy converters could be installed preventing the devastation of the rich ecosphere renown in the region

Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza

Abstract Introduction Human host immune response following infection with the new variant of A/H1N1 pandemic influenza virus (nvH1N1) is poorly understood. We utilize here systemic cytokine and antibody levels in evaluating differences in early immune response in both mild and severe patients infected with nvH1N1. Methods We profiled 29 cytokines and chemokines and evaluated the haemagglutination inhibition activity as quantitative and qualitative measurements of host immune responses in serum obtained during the first five days after symptoms onset, in two cohorts of nvH1N1 infected patients. Severe patients required hospitalization (n = 20), due to respiratory insufficiency (10 of them were admitted to the intensive care unit), while mild patients had exclusively flu-like symptoms (n = 15). A group of healthy donors was included as control (n = 15). Differences in levels of mediators between groups were assessed by using the non parametric U-Mann Whitney test. Association between variables was determined by calculating the Spearman correlation coefficient. Viral load was performed in serum by using real-time PCR targeting the neuraminidase gene. Results Increased levels of innate-immunity mediators (IP-10, MCP-1, MIP-1β), and the absence of anti-nvH1N1 antibodies, characterized the early response to nvH1N1 infection in both hospitalized and mild patients. High systemic levels of type-II interferon (IFN-γ) and also of a group of mediators involved in the development of T-helper 17 (IL-8, IL-9, IL-17, IL-6) and T-helper 1 (TNF-α, IL-15, IL-12p70) responses were exclusively found in hospitalized patients. IL-15, IL-12p70, IL-6 constituted a hallmark of critical illness in our study. A significant inverse association was found between IL-6, IL-8 and PaO2 in critical patients. Conclusions While infection with the nvH1N1 induces a typical innate response in both mild and severe patients, severe disease with respiratory involvement is characterized by early secretion of Th17 and Th1 cytokines usually associated with cell mediated immunity but also commonly linked to the pathogenesis of autoimmune/inflammatory diseases. The exact role of Th1 and Th17 mediators in the evolution of nvH1N1 mild and severe disease merits further investigation as to the detrimental or beneficial role these cytokines play in severe illness

Host adaptive immunity deficiency in severe pandemic influenza

INTRODUCTION: Pandemic A/H1N1/2009 influenza causes severe lower respiratory complications in rare cases. The association between host immune responses and clinical outcome in severe cases is unknown. METHODS: We utilized gene expression, cytokine profiles and generation of antibody responses following hospitalization in 19 critically ill patients with primary pandemic A/H1N1/2009 influenza pneumonia for identifying host immune responses associated with clinical outcome. Ingenuity pathway analysis 8.5 (IPA) (Ingenuity Systems, Redwood City, CA) was used to select, annotate and visualize genes by function and pathway (gene ontology). IPA analysis identified those canonical pathways differentially expressed (P < 0.05) between comparison groups. Hierarchical clustering of those genes differentially expressed between groups by IPA analysis was performed using BRB-Array Tools v.3.8.1. RESULTS: The majority of patients were characterized by the presence of comorbidities and the absence of immunosuppressive conditions. pH1N1 specific antibody production was observed around day 9 from disease onset and defined an early period of innate immune response and a late period of adaptive immune response to the virus. The most severe patients (n = 12) showed persistence of viral secretion. Seven of the most severe patients died. During the late phase, the most severe patient group had impaired expression of a number of genes participating in adaptive immune responses when compared to less severe patients. These genes were involved in antigen presentation, B-cell development, T-helper cell differentiation, CD28, granzyme B signaling, apoptosis and protein ubiquitination. Patients with the poorest outcomes were characterized by proinflammatory hypercytokinemia, along with elevated levels of immunosuppressory cytokines (interleukin (IL)-10 and IL-1ra) in serum. CONCLUSIONS: Our findings suggest an impaired development of adaptive immunity in the most severe cases of pandemic influenza, leading to an unremitting cycle of viral replication and innate cytokine-chemokine release. Interruption of this deleterious cycle may improve disease outcome.The study was scientifically sponsored by the Spanish Society for Critical Care Medicine (SEMICYUC). Funding: MICCIN-FIS/JCYL-IECSCYL-SACYL (Spain): Programa de Investigación Comisionada en Gripe, GR09/0021-EMER07/050- PI081236-RD07/0067. CIHR-NIH-Sardinia Recherché-LKSF Canada support DJK.S

Expression of Colonization Factor CS5 of Enterotoxigenic Escherichia coli (ETEC) Is Enhanced In Vivo and by the Bile Component Na Glycocholate Hydrate

Enterotoxigenic Escherichia coli (ETEC) is an important cause of acute watery diarrhoea in developing countries. Colonization factors (CFs) on the bacterial surface mediate adhesion to the small intestinal epithelium. Two of the most common CFs worldwide are coli surface antigens 5 and 6 (CS5, CS6). In this study we investigated the expression of CS5 and CS6 in vivo, and the effects of bile and sodium bicarbonate, present in the human gut, on the expression of CS5. Five CS5+CS6 ETEC isolates from adult Bangladeshi patients with acute diarrhoea were studied. The level of transcription from the CS5 operon was approximately 100-fold higher than from the CS6 operon in ETEC bacteria recovered directly from diarrhoeal stool without sub-culturing (in vivo). The glyco-conjugated primary bile salt sodium glycocholate hydrate (NaGCH) induced phenotypic expression of CS5 in a dose-dependent manner and caused a 100-fold up-regulation of CS5 mRNA levels; this is the first description of NaGCH as an enteropathogenic virulence inducer. The relative transcription levels from the CS5 and CS6 operons in the presence of bile or NaGCH in vitro were similar to those in vivo. Another bile salt, sodium deoxycholate (NaDC), previously reported to induce enteropathogenic virulence, also induced expression of CS5, whereas sodium bicarbonate did not