Risk of Seven-Day Worsening and Death: A New Clinically Derived COVID-19 Score

This monocentric, retrospective, two-stage observational study aimed to recognize the risk factors for a poor outcome in patients hospitalized with SARS-CoV-2 infection, and to develop and validate a risk score that identifies subjects at risk of worsening, death, or both. The data of patients with SARS-CoV-2 infection during the first wave of the pandemic were collected and analyzed as a derivation cohort. Variables with predictive properties were used to construct a prognostic score, which was tried out on a validation cohort enrolled during the second wave. The derivation cohort included 494 patients; the median age was 62 and the overall fatality rate was 22.3%. In a multivariable analysis, age, oxygen saturation, neutrophil-to-lymphocyte ratio, C-reactive protein and lactate dehydrogenase were independent predictors of death and composed the score. A cutoff value of 3 demonstrated a sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of 93.5%, 68.5%, 47.4% and 97.2% for death, and 84.9%, 84.5%, 79.6% and 87.9% for worsening, respectively. The validation cohort included 415 subjects. The score application showed a Se, Sp, PPV and NPV of 93.4%, 61.6%, 29.5% and 98.1% for death, and 81%, 76.3%, 72.1% and 84.1% for worsening, respectively. We propose a new clinical, easy and reliable score to predict the outcome in hospitalized SARS-CoV-2 patients

Serum ferritin is an independent risk factor for acute respiratory distress syndrome in COVID-19

Increased ferritin levels could be indicative of a strong inflammatory reaction in COVID-19 and recent studies suggest that increased levels of circulating ferritin levels play a critical role by contributing to the development of a cytokine storm. In this regard, ferritin evaluation could be an early, available and easy to use screening tool to assess the disease severity at the first admission in the emergency department. This test might be of crucial importance for the timely identification of patients at higher risk of an adverse outcome

Resurrection of Anopheles goeldii from synonymy with Anopheles nuneztovari (Diptera, Culicidae) and a new record for Anopheles dunhami in the Brazilian Amazon

Nucleotide sequences of the internal transcribed spacer 2 (ITS2) rDNA and partial sequences of the cytochrome coxidase subunit I (COI) mtDNA and white gene nDNA were obtained from specimens of Anopheles nuneztovari A collected in Macapá (state of Amapá), Óbidos, Prainha and Almeirim (state of Pará), Itacoatiara and Parintins (state of Amazonas), Brazil, and compared with previously published sequences of A. nuneztovari s.l. Results of the Bayesian phylogenetic analyses performed using either COI or combined ITS2, COI and white gene sequences suggest that An. nuneztovari B/C is distinct from specimens obtained in the Amazonas/Solimões River basin. Anopheles goeldii, currently in synonymy with An. nuneztovari, was described from individuals collected in Belterra (= Fordlândia) in the Tapajós River, state of Pará, Southern Amazonas River. Morphological comparisons of the characteristics of the male genitalia indicated that An. nuneztovari A and An. goeldii are similar but distinct from An. nuneztovariB/C by the apex of the aedeagus. In considering the results of the phylogenetic analyses and morphological comparisons, An. goeldii is resurrected from synonymy with An. nuneztovari. Additionally, Anopheles dunhamiis reported for the first time in Parintins. This species can be distinguished from An. goeldiiby characters of the male genitalia and molecular dataFAPESP 05/53973-0, CNPq 472485/2006-

LA FLORA E LA MESOFAUNA DI SATRIANUM

Il sito di Satrianum si dispone tra le due vallate del Melandro e del Noce, in connessione con un’articolata rete idrografica facente capo ai fiumi Agri e Basento . Tutta l’area che circonda l’altura, è caratterizzata da terreni che si dispiegano tra gli 800 e i 600 m. s.l.m., e presenta un habitat montano ideale per le colture cerealicole, per le distese boschive e per la pastorizia. Notizie del sito si attestano fin dall’età altomedievale (Agiografia di San Laviero, 1162). In età normanna la città aveva un dominus e la sede vescovile come testimoniano le fonti documentarie. L’impianto insediativo si presenta racchiuso da mura di cinta, con all’ interno, oltre alle strutture abitative, i due poli del potere politico e religioso: la torre, a pianta quadrata, ubicata in posizione decentrata rispetto all’insediamento, e la cattedrale. L’abbandono di Satrianum avvenne nel corso del XV secolo, probabilmente a seguito di un grave evento sismico ma continuò ad essere frequentato in maniera sporadica fino al XVIII secolo. Nell’ottica di comprendere e ricreare un ambiente passato è stata effettuata una ricognizione e l’identificazione della flora e della mesofauna esistente. Alla fine è stato realizzato un erbario e un insettario

Usefulness of bronchoalveolar lavage in suspect COVID-19 repeatedly negative swab test and interstitial lung disease

The diagnosis of coronavirus disease 2019 (COVID-19) relies on nasopharyngeal swab, which shows a 20–30% risk of false negativity [1]. Bronchoalveolar lavage (BAL) is reported to be useful in patients with pulmonary interstitial infiltrates on high-resolution computed tomography (HRCT). We investigated the usefulness of BAL in symptomatic patients with positive HRCT and a repeatedly negative swab test (‘grey zone’)

Halpha3: Halpha imaging survey of HI selected galaxies from ALFALFA

We present Halpha3 (acronym for Halpha-alpha-alpha), an Halpha narrow-band imaging survey of ~400 galaxies selected from the HI Arecibo Legacy Fast ALFA Survey (ALFALFA) in the Local Supercluster, including the Virgo cluster. By using hydrogen recombination lines as a tracer of recent star formation, we aim to investigate the relationships between atomic neutral gas and newly formed stars in different environments (cluster and field), morphological types (spirals and dwarfs), and over a wide range of stellar masses (~10^7.5-10^11.5 Msun). We image in Halpha+[NII] all the galaxies that contain more than 10^7 Msun of neutral atomic hydrogen in the sky region 11^h < R.A. <16^h 4^o < Dec. <16^o; 350< cz <2000 km/s using the San Pedro Martir 2m telescope. This survey provides a complete census of the star formation in HI rich galaxies of the local universe. We present the properties of the galaxy sample, together with Halpha fluxes and equivalent widths. We find an excellent agreement between the fluxes determined from our images in apertures of 3 arcsec diameter and the fluxes derived from the SDSS spectral database. From the Halpha fluxes corrected for galactic and internal extinction and for [NII] contamination we derive the global star formation rates (SFRs).Comment: 22 pages, 8 figures. A&A accepted. Atlas available upon reques

Survivorship of Anopheles darlingi (Diptera: Culicidae) in Relation with Malaria Incidence in the Brazilian Amazon

We performed a longitudinal study of adult survival of Anopheles darlingi, the most important vector in the Amazon, in a malarigenous frontier zone of Brazil. Survival rates were determined from both parous rates and multiparous dissections. Anopheles darlingi human biting rates, daily survival rates and expectation of life where higher in the dry season, as compared to the rainy season, and were correlated with malaria incidence. The biting density of mosquitoes that had survived long enough for completing at least one sporogonic cycle was related with the number of malaria cases by linear regression. Survival rates were the limiting factor explaining longitudinal variations in Plasmodium vivax malaria incidence and the association between adult mosquito survival and malaria was statistically significant by logistic regression (P<0.05). Survival rates were better correlated with malaria incidence than adult mosquito biting density. Mathematical modeling showed that P. falciparum and P. malariae were more vulnerable to changes in mosquito survival rates because of longer sporogonic cycle duration, as compared to P. vivax, which could account for the low prevalence of the former parasites observed in the study area. Population modeling also showed that the observed decreases in human biting rates in the wet season could be entirely explained by decreases in survival rates, suggesting that decreased breeding did not occur in the wet season, at the sites where adult mosquitoes were collected. For the first time in the literature, multivariate methods detected a statistically significant inverse relation (P<0.05) between the number of rainy days per month and daily survival rates, suggesting that rainfall may cause adult mortality

LKB1 is an essential regulator of spermatozoa release during spermiation in the mammalian testis

LKB1 acts as a master upstream protein kinase regulating a number of kinases involved in diverse cellular functions. Recent studies have suggested a role for LKB1 in male fertility. Male mice with reduced total LKB1 expression, including the complete absence of the major splice variant in testis (LKB1(S)), are completely infertile. We sought to further characterise these mice and determine the mechanism underlying this infertility. This involved expression studies of LKB1 in developing germ cells, morphological analysis of mature spermatozoa and histological studies of both the testis and epididymis using light microscopy and transmission electron microscopy. We conclude that a defect in the release of mature spermatids from the seminiferous epithelium (spermiation) during spermatozoan development is a major cause of the infertility phenotype. We also present evidence that this is due, at least in part, to defects in the breakdown of the junctions, known as ectoplasmic specialisations, between the sertoli cells of the testis epithelium and the heads of the maturing spermatids. Overall this study uncovers a critical role for LKB1 in spermiation, a highly regulated, but poorly understood process vital for male fertility