56 research outputs found

    Specific features of Bazhenov suite sediments in south-eastern Nurolsk sedimentary basin (Tomsk Oblast)

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    The specific sediment features in Georgiev (J[3]kmgr), Bazhenov (J3vbg) and Kulomzin (K1bkl) suites, exposed by drilling in the S-E Nurolsk depression (Tomsk Oblast), were defined and described via petrographic, X-ray diffraction and fluorescence-microscopy analysis methods. The classification of agrillites was identified, the structure-texture features, composition, voids and bitumen types and their distribution were determined. It was defined that Bazhenov suite argillites are characteristic of fine-dispersion, high biogenic silica content and scattered organic matter, enriched multi-composite syngenetic bitumen (from light to resin-asphaltine), as well as fractured surface where the migration of light bitumen occurs

    The mRubyFT Protein, Genetically Encoded Blue-to-Red Fluorescent Timer.

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    peer reviewedGenetically encoded monomeric blue-to-red fluorescent timers (mFTs) change their fluorescent color over time. mCherry-derived mFTs were used for the tracking of the protein age, visualization of the protein trafficking, and labeling of engram cells. However, the brightness of the blue and red forms of mFTs are 2-3- and 5-7-fold dimmer compared to the brightness of the enhanced green fluorescent protein (EGFP). To address this limitation, we developed a blue-to-red fluorescent timer, named mRubyFT, derived from the bright mRuby2 red fluorescent protein. The blue form of mRubyFT reached its maximum at 5.7 h and completely transformed into the red form that had a maturation half-time of 15 h. Blue and red forms of purified mRubyFT were 4.1-fold brighter and 1.3-fold dimmer than the respective forms of the mCherry-derived Fast-FT timer in vitro. When expressed in mammalian cells, both forms of mRubyFT were 1.3-fold brighter than the respective forms of Fast-FT. The violet light-induced blue-to-red photoconversion was 4.2-fold less efficient in the case of mRubyFT timer compared to the same photoconversion of the Fast-FT timer. The timer behavior of mRubyFT was confirmed in mammalian cells. The monomeric properties of mRubyFT allowed the labeling and confocal imaging of cytoskeleton proteins in live mammalian cells. The X-ray structure of the red form of mRubyFT at 1.5 Å resolution was obtained and analyzed. The role of the residues from the chromophore surrounding was studied using site-directed mutagenesis

    Enthalpy of solvation correlations for organic solutes and gases dissolved in 1-propanol and tetrahydrofuran

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    This article discusses the enthalpy of solvation correlations for organic solutes and gases dissolved in 1-propanol and tetrahydrofuran

    Blasthole drilling in a thin vein of quartzite-like sandstone

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    Implementation of the asynchronous signals reception method with the direct sequence spread spectrum

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    The physical and logical organization of most existing communication systems provides for additional options (transmission of known preambles, the presence of an additional synchronization channel) that simplify synchronization. At the same time, in the practical implementation of radio communication systems, it is necessary to solve the problems of developing additional synchronization mechanisms that can reduce the time it takes to synchronize to reduce energy consumption and increase channel capacity. Thus, the existing problem of the asynchrony of the generators of physically remote radio stations is relevant. One of the possible solutions is asynchronous signal reception with the direct sequence spread spectrum. It allows converting the original binary signal into a pseudo-random sequence for modulating the carrier. The current article is devoted to the characterization of this method and has the following items presented: the transmitter and receiver block diagram, sufficient to explain the proposed method; methods for compensating for effects arising from the asynchrony of the transmitter and receiver generators. Since synchronization is generated according to the correlation peaks that correspond to the data bits, it is possible to set the frequency of the chips on the transmitter to jitter artificially and supplement the pseudo-random sequence with several random chips, which complicates the unauthorized detection of the transmitted signal
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