1,180 research outputs found
Acumulación de Cu y Zn por célular microalgales marinas de Nannochloropsis gaditana (Eustigmatophyceae) inmovilizadas en alginato de calcio
13 páginas, 5 figuras, 2 tablas.[EN] Different experiments about the accumulation capacity for copper and zinc were carried out on the marine microalgae
Nannochloropsis gaditana Lubián (Eustigmatophyceae). A 24-hour study of the evolution of accumulated metal in the beads
revealed two possible accumulation phases. Accumulation differences between free and immobilized microalgal cells were
investigated finding no differences for copper, and little differences for zinc. Free cells accumulated practically 100% Cu or Zn in
the media under experimental conditions. Experiments in order to compare the accumulation capacity of living vs. dead cells were
designed too, obtaining the largest accumulation levels for both metals in the beads containing immobilized living microalgae. In
experiments carried out in continuous-flow reactors, beads with entrapped cells showed to be more efficient removing Cu and Zn
than beads without cells. In all the experiments, the calcium alginate beads showed strong affinity for Cu. Similar results were
obtained when accumulation by packed beads in columns was tested, but efficacy was higher if this design was used (beads with
cells retain 80% of Cu introduced in the column).[ES] Se han realizado distintos experimentos sobre la capacidad de acumulación de cobre y zinc por parte de la microalga marina
Nannochloropsis gaditana Lubián (Eustigmatophyceae). Un estudio de la evolución del metal acumulado durante 24 horas reveló
la existencia de dos posibles fases de acumulación. Se investigaron las diferentes capacidades de acumulación de células
microalgales libres e inmovilizadas, no encontrándose diferencias para el cobre y pequeñas diferencias para el zinc. Las células
libres acumularon prácticamente el 100% del Cu o Zn presentes en el medio, en las condiciones experimentales. Se desarrollaron
experimentos encaminados a determinar la capacidad de acumulación de estos metales por parte de algas inmovilizadas vivas
frente a la de algas inmovilizadas muertas, encontrándose los niveles acumulativos más altos para ambos metales en las gotas de
alginato que contenían algas vivas. En experimentos realizados en reactores de flujo continuo, las gotas de alginato con células
inmovilizadas en su interior demostraron ser más eficientes en cuanto a la retirada de Cu y Zn del medio que las gotas sin algas.
En todos los experimentos realizados se observa una fuerte afinidad del Cu por el alginato de calcio. Se obtuvieron resultados
similares cuando se ensayó la acumulación de metales en gotas de alginato dispuestas en columnas llenas, aunque la eficacia fue
mayor cuando se usó este último diseño (las gotas de alginato con células retuvieron el 80% del Cu introducido en la columna).Peer reviewe
Influence of metals and metalloids on the composition and fluorescence quenching of the extracellular polymeric substances produced by the polymorphic fungus <i>Aureobasidium pullulans</i>
Aureobasidium pullulansis a ubiquitous and widely distributed fungus in the environment, and exhibits substantial tolerance against toxic metals. However, the interactions between metals and metalloids with the copious extracellular polymeric substances (EPS) produced byA. pullulansand possible relationships to tolerance are not well understood. In this study, it was found that mercury (Hg) and selenium (Se), as selenite, not only significantly inhibited growth ofA. pullulansbut also affected the composition of produced EPS. Lead (Pb) showed little influence on EPS yield or composition. The interactions of EPS fromA. pullulanswith the tested metals and metalloids depended on the specific element and their concentration. Fluorescence intensity measurements of the EPS showed that the presence of metal(loid)s stimulated the production of extracellular tryptophan-like and aromatic protein-like substances. Examination of fluorescence quenching and calculation of binding constants revealed that the fluorescence quenching process for Hg; arsenic (As), as arsenite; and Pb to EPS were mainly governed by static quenching which resulted in the formation of a stable non-fluorescent complexes between the EPS and metal(loid)s. Se showed no significant interaction with the EPS according to fluorescence quenching. These results provide further understanding of the interactions between metals and metalloids and EPS produced by fungi and their contribution to metal(loid) tolerance
Biotransformation of lanthanum by Aspergillus niger
Lanthanum is an important rare earth element and has many applications in modern electronics and catalyst manufacturing. However, there exist several obstacles in the recovery and cycling of this element due to a low average grade in exploitable deposits and low recovery rates by energy-intensive extraction procedures. In this work, a novel method to transform and recover La has been proposed using the geoactive properties of Aspergillus niger. La-containing crystals were formed and collected after A. niger was grown on Czapek-Dox agar medium amended with LaCl 3. Energy-dispersive X-ray analysis (EDXA) showed the crystals contained C, O, and La; scanning electron microscopy revealed that the crystals were of a tabular structure with terraced surfaces. X-ray diffraction identified the mineral phase of the sample as La 2(C 2O 4) 3·10H 2O. Thermogravimetric analysis transformed the oxalate crystals into La 2O 3 with the kinetics of thermal decomposition corresponding well with theoretical calculations. Geochemical modelling further confirmed that the crystals were lanthanum decahydrate and identified optimal conditions for their precipitation. To quantify crystal production, biomass-free fungal culture supernatants were used to precipitate La. The results showed that the precipitated lanthanum decahydrate achieved optimal yields when the concentration of La was above 15 mM and that 100% La was removed from the system at 5 mM La. Our findings provide a new aspect in the biotransformation and biorecovery of rare earth elements from solution using biomass-free fungal culture systems. </p
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Avoiding Breast Animation Deformity with Pectoralis-Sparing Subcutaneous Direct-to-Implant Breast Reconstruction
Summary: There is renewed interest in sparing the pectoralis major muscle in implant breast reconstruction, placing the implant in the subcutaneous position. This advance is made reliable by improvements in mastectomy skin flap quality, evolution of technique, and increased awareness of breast animation deformity. This retrospective review presents 13 patients (23 breasts) reconstructed immediately with placing the definitive implant in the subcutaneous space without disruption of the underlying chest muscles. None of the 13 patients had breast animation deformity postoperatively. One patient experienced an early hematoma, and 3 patients had small seromas that resolved uneventfully. One morbidly obese patient undergoing chemotherapy using a nearby chest port experienced infection requiring implant removal. This study describes an early experience, demonstrates feasibility, and discusses patient selection considerations that are important because we continue to evolve breast reconstruction approaches
Structure-guided design and optimization of small molecules targeting the protein-protein interaction between the von hippel-lindau (VHL) E3 ubiquitin ligase and the hypoxia inducible factor (HIF) alpha subunit with in vitro nanomolar affinities
E3 ubiquitin ligases are attractive targets in the ubiquitin-proteasome system, however, the development of small-molecule ligands has been rewarded with limited success. The von Hippel-Lindau protein (pVHL) is the substrate recognition subunit of the VHL E3 ligase that targets HIF-1α for degradation. We recently reported inhibitors of the pVHL:HIF-1α interaction, however they exhibited moderate potency. Herein, we report the design and optimization, guided by X-ray crystal structures, of a ligand series with nanomolar binding affinities
Low temperature heat capacity of fullerite C60 doped with nitrogen
The heat capacity Cm of polycrystalline fullerite C60 doped with nitrogen has
been measured in the temperature interval 2 - 13 K. The contributions to heat
capacity from translational lattice vibrations (Debye contribution),
orientational vibrations of the C60 molecules (Einstein contribution) and from
the motion of the N2 molecules in the octahedral cavities of the C60 lattice
have been estimated. However, we could not find (beyond the experimental error
limits) any indications of the first - order phase transformation that had been
detected earlier in the dilatometric investigation of the orientational N2-C60
glass. A possible explanation of this fact is proposed.Comment: 4 pages, 2 figures, to be published in Fiz. Nizk. Temp. (Low Temp.
Phys.
Multiple cardiac biomarkers to improve prediction of cardiovascular events:Findings from the Generation Scotland Scottish Family Health Study
Background: Many studies have investigated whether single cardiac biomarkers improve cardiovascular risk prediction for primary prevention but whether a combined approach could further improve risk prediction is unclear. We aimed to test a sex-specific combined cardiac biomarker approach for cardiovascular risk prediction. Methods: In the Generation Scotland Scottish Family Health Study, N-terminal pro-B-type natriuretic peptide (NT-proBNP), growth differentiation factor-15 (GDF-15), cardiac troponin I (cTnI), cardiac troponin T (cTnT), and C-reactive protein (CRP) were measured in stored serum using automated immunoassays. Sex-specific Cox models that included SCORE2 risk factors evaluated addition of single and combined biomarkers for prediction of major adverse cardiovascular events (MACE). Combined biomarker models were compared to a baseline model that included SCORE2 factors.Results: The study population comprised 18,383 individuals (58.9% women, median age of 48 years [25th-75th percentile, 35-58 years]). During the median follow up of 11.6 (25th-75th percentile, 10.8-13.0) years, MACE occurred in 942 (5.1%) individuals. The greatest increase in discrimination with addition of individual biomarkers to base model was for women GDF-15 and for men NT-proBNP (change in c-index: +0.010 for women and +0.005 for men). For women, combined biomarker models that included GDF-15 and NT-proBNP (+0.012) or GDF-15 and cTnI (+0.013), but not CRP or cTnT, further improved discrimination. For men, combined biomarker models that included NT-proBNP and GDF-15 (+0.007), NT-proBNP and cTnI (+0.006), or NT-proBNP and CRP (+0.008), but not cTnT, further improved discrimination. Conclusions: A combined biomarker approach, particularly the use of GDF-15, NT-proBNP and cTnI, further refined cardiovascular risk estimates.<br/
Behavioural risk factors in two generations of non-Western migrants: do trends converge towards the host population?
Migrant mortality does not conform to a single pattern of convergence towards prevalence rates in the host population. To understand better how migrant mortality develops, it is necessary to further investigate how the underlying behavioural determinants change following migration. We studied whether the prevalence of behavioural risk factors over two generations of Turkish and Moroccan migrants converge towards the prevalence rates in the Dutch population. From a random sample from the population register of Amsterdam, 291 Moroccan and 505 Turkish migrants, aged 15–30, participated in a structured interview that included questions on smoking, alcohol consumption, physical inactivity and weight/height. Data from the Dutch population were available from Statistics Netherlands. By calculating age-adjusted Odds Ratio’s, prevalence rates among both generations were compared with prevalence rates in the host population for men and women separately. We found indications of convergence across generations towards the prevalence rates in the host population for smoking in Turkish men, for overweight in Turkish and Moroccan women and for physical inactivity in Turkish women. Alcohol consumption, however, remained low in all subgroups and did not converge towards the higher rates in the host population. In addition, we found a reversed trend among Turkish women regarding smoking: the second generation smoked significantly more, while the first generation did not differ from ethnic Dutch. In general, behavioural risk factors in two generations of non-Western migrants in the Netherlands seem to converge towards the prevalence rates in the Dutch population. However, some subgroups and risk factors showed a different pattern
Academic freedom: in justification of a universal ideal
This paper examines the justification for, and benefits of, academic freedom to academics, students, universities and the world at large. The paper surveys the development of the concept of academic freedom within Europe, more especially the impact of the reforms at the University of Berlin instigated by Wilhelm von Humboldt. Following from this, the paper examines the reasons why the various facets of academic freedom are important and why the principle should continue to be supported
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