Impacts of Different Water Pollution Sources on Antioxidant Defense Ability in Three Aquatic Macrophytes in Assiut Province, Egypt

The present study was undertaken to evaluate the impacts of surface water pollution with wastes coming from sewage effluents (Site 2), agricultural runoff (Site 4) and oils and detergents factory (Site 3) on the stability of leaf membrane (measured as injury %), hydrogen peroxide (H2O2), ascorbic acid (Asc A), lipid peroxidation, chlorophyll (Chl) content, soluble sugars (SS), soluble proteins (SP) and total free amino acids (TAA) of Cyperus alopeucroides, Persicaria salicifolia and Echinochloa stagnina. Concentration of H2O2, MDA and TAA were higher in the three plants collected from polluted sites as compared with those of plants grown in control Nile site (Site1). The opposite was true for Asc A, SS and SP where their concentrations reduced significantly in response to water pollution. Leaf membrane was more damaged (high injury %) in plants exposed to wastes from different sources than in plants growing at control site. The results of this study indicated that water pollution reduced the oxidative defense abilities in the three plants through reduction of Asc A activities, enhancement of H2O2 production and increasing MDA accumulation. In addition it impaired the metabolic activity through lowering the SS and SP contents and enhancement of TAA accumulation and increase membrane injury. The over production of hydrogen peroxide by the studied aquatic plants under water pollution could be used as an oxygen source needed to oxidize the more resistant organic and inorganic pollutants and used for pollution control and municipal and industrial wastewater treatment

Acrylamide-induced adverse cerebellar changes in rats: possible oligodendrogenic effect of omega 3 and green tea

Background: Humans are widely exposed to acrylamide (ACR) and its neurotoxicity is a significant public health issue attracting wide attention. The aim of the study was to investigate ACR-induced adverse cerebellar changes in rats and study the possible oligodendrogenic effect of omega 3 and green tea. Materials and methods: Twenty-four adult albino rats weighing 150–200 g were randomly divided into four equal groups (6 rats each): control group (Group I), the rats that received ACR 45 mg/kg/day (Group II), the rats that received ACR concomitant with omega 3 at a dosage of 200 mg/kg/day (Group III), the rats that received ACR concomitant with green tea dissolved in drinking water at a dosage of 5 g/L (Group IV). The rats were euthanized after 8 weeks of the experiment. Malondialdehyde (MDA) and glutathione (GSH) were measured in cerebellar homogenates. Sections of 5 μm thickness from specimens from the cerebellum were stained with haematoxylin and eosin, silver stain and immunohistochemical stains: platelet-derived growth factor alpha (PDGFα; for oligodendrocytes), glial fibrillary acidic protein (GFAP; for astrocytes) and BCL2 (antiapoptotic). Results: Omega 3 and green tea had improved MDA and GSH as compared to the ACR group. Histologically, the ACR group showed variable degrees of cellular degeneration. Omega 3 had induced oligodendrogenesis in Group III. The optical density of silver stain was significantly (p < 0.05) increased in Groups III and IV as compared to the ACR group. Area per cent of positive PDGFα was significantly increased in the ACR + omega 3 group as compared to the ACR group. Area per cent of positive GFAP was significantly decreased in Groups III and IV as compared to the ACR group. Area per cent of positive BCL2 was significantly increased in the omega 3-trated group as compared to the ACR group. Conclusions: Concomitant administration of omega 3 or green tea with ACR might mitigate the adverse cerebellar changes caused by ACR thanks to an oligodendrogenic effect of omega 3

Evaluation of Serum Gonadotropin and Prolactin Level among Sudanese Patients with Chronic Renal Failure

Background: Generally, patients on hemodialysis for chronic renal failure also have endocrine defects and sexual function disorders. In this study, we aimed to assess the serum prolactin (PRL), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in patients with chronic renal failure. Methods: This hospital-based case–control study was conducted at Jabal Aulia Teaching Hospital, Khartoum, Sudan. The study was carried out between August 2019 and February 2020. A total of 100 subjects were enrolled – 50 chronic renal failure patients and 50 as controls. The serum hormones were estimated using Tosoh 360. SPSS version 25 was used to analyze the results. Results: The serum PRL, LH, and FSH were significantly increased among chronic renal failure patients than their healthy counterparts (p-value = 0.000). The age of patients was positive correlated with plasma hormones, PRL (r = 0.332, p = 0.001), LH (r = 0.387, p = 0.000), and FSH (r = 0.320, p = 0.001). No correlation was found between the duration of the disease and serum hormones. Conclusion: Patients with chronic renal failure had a highly significant increase of serum PRL, LH, and FSH and also the age of the patients was positively correlated with serum hormones. Keywords: chronic renal failure, prolactin, gonadotropin, hemodialysi

Modeling Survival Distributions For Certain Real-World Applications Using Nonparametric Hypothesis Testing

By analyzing the failure behavior of the recorded survival data, we expect to analyze the different processing strategies or functionality of the tools or systems used in this nonparametric statistical test. It is anticipated that the test data would either behave exponentially or like the NBUE Property. If the survival results are NBUE, it is anticipated that the suggested treatment plan would be successful. Contrarily, As shown in the application section, if the data are exponential, the suggested treatment plan wont have any positive or negative consequences on patients. We evaluated the suggested tests efficacy and power for both complete and censored data, compared the outcomes with those of existing tests, and then applied the test to a variety of real-world data to demonstrate its validity

Stellar parameters of Be stars observed with X-shooter

Aims. The X-shooter archive of several thousand telluric star spectra was skimmed for Be and Be-shell stars to derive the stellar fundamental parameters and statistical properties, in particular for the less investigated late type Be stars, and the extension of the Be phenomenon into early A stars. Methods. An adapted version of the BCD method is used, utilizing the Balmer discontinuity parameters to determine effective temperature and surface gravity. This method is optimally suited for late B stars. The projected rotational velocity was obtained by profile fitting to the Mg ii lines of the targets, and the spectra were inspected visually for the presence of peculiar features such as the infrared Ca ii triplet or the presence of a double Balmer discontinuity. The Balmer line equivalent widths were measured, but due to uncertainties in determining the photospheric contribution are useful only in a subsample of Be stars for determining the pure emission contribution. Results. A total of 78 Be stars, mostly late type ones, were identified in the X-shooter telluric standard star archive, out of which 48 had not been reported before. The general trend of late type Be stars having more tenuous disks and being less variable than early type ones is confirmed. The relatively large number (48) of relatively bright (V > 8.5) additional Be stars casts some doubt on the statistics of late type Be stars; they are more common than currently thought: The Be/B star fraction may not strongly depend on spectral subtype.Comment: Accepted for publication in A&

Stimulated emission from ZnO thin films with high optical gain and low loss

Stimulated surface- and edge-emission were investigated for ZnO thin films grown epitaxially by pulsed laser deposition. The lasing threshold was 0.32 MW/cm2 for surface pumping and 0.5 MW/cm2 for edge pumping, which is significantly lower than thresholds observed previously. A modified variable stripe length method was used to measure the gain, which was 1369 cm-1 for N-band emission. Losses were measured using the shifting excitation spot method and values of 6.2 cm-1 and 6.3 cm-1 were found for the N-band and P-band, respectively. The measured gain and loss were the highest and lowest (respectively) ever reported for ZnO films

Evaluation of LL 37 Lipoprotein as Innate Immunity Marker among Sudanese Patients Cutaneous Leishmania

Background: The leishmaniasis is a group of diseases with a broad range of clinical manifestations caused by several species of parasites belonging to the genus Leishmania. LL-37/hCAP18, the only cathelicidin in human, is expressed as an 18-kDa preproprotein. The most prominent function of cathelicidins is their ability to inhibit propagation of a diverse range of microorganisms, which occurs at a micromolar range. Aim: The study was aimed to evaluate the LL37 plasma level in Leishmania Sudanese patients. Materials and Methods: In a case-control study, three hundred subjects were enrolled (200 as case and 100 controls). Five ml. of venous blood was collected in EDTA container, then plasma was obtained and stored frozen at -80ºC. LL 37 was estimated using competitive ELISA. The data were analyzed using SPSS version 21. Results: The results revealed that 115 (57%) of Leishimania patients were male and 85 (43%) were female. Plasma LL 37 level was significantly increased in Leishmania patients (1.30±0.71) compared to the control (0.21±0.20) with (p-value 0.000). Conclusion: Leishmania patients had higher levels of plasma LL37, suggesting effective anti-microbial immunity process enhancing healing of cutaneous leishmaniasis. Keywords:  Cathelicidins; LL37, Innate Immunity, Leishmania , Sudan

Primary torsion of the greater omentum: An overlooked cause of acute abdomen

Background/purpose Primary torsion of the greater omentum is an uncommon cause of acute abdomen in children. It was estimated to be found in 0.1% of cases operated for acute appendicitis and is almost impossible to be diagnosed preoperatively. Surgical excision of the infarcted omentum is the treatment of choice. In this study, we aimed to highlight the importance of suspecting primary omental torsion when operating upon a child presenting with a picture of acute appendicitis with normal appearing appendix intraoperatively.Patients and methods Through the period from June 2009 to May 2016, medical records of patients who had definite diagnosis of primary omental torsion were retrospectively reviewed. Clinical presentations, laboratory findings, imaging studies results, intraoperative findings, and histopathological findings were reviewed.Results During the specified time period, out of 1344 patients operated upon for acute appendicitis in our department, only four patients proved to have primary torsion of the greater omentum. All the patients were obese with clinical picture mimicking acute appendicitis. The postoperative histopathological examination showed normal appendix and ischemic necrosis of the resected omental segment.Conclusion Inspection of the greater omentum is essential when finding a normal appearing appendix in any case operated for suspicion of acute appendicitis. Keywords: acute abdomen, appendix, greater omentum, omental torsio

Effects of administration of 10 nm or 50 nm gold nanoparticles (AuNPs) on blood profile, liver and kidney functions in male albino rats

This work aimed to investigate the effect of acute and chronic administration of gold nanoparticles (GNPs) on liver and kidney functions, blood glucose concentration, lipid profile, and haematological parameters in male albino rats. Two experiments were conducted. In acute study: Fifty-four adult mature male rats were randomly assigned into three equal groups (18 per group). Group 1 (control group): in which rats were received intramuscular (i.m) injection of 1 ml normal saline 0.9%. Group 2 (50 nm GNPs group): rats were i.m. injected with a single dose of 75 µg 50 nm GNPs/kg body weight (bwt). In Group 3 (10 nm GNPs group): rats were i.m. injected with a single dose of 75 µg 10 nm GNPs/kg bwt. In chronic study: Eighteen adult male rats were randomly divided into three equal groups (6 per group). Group І (control): rats were intramuscular (i.m) repeatedly injected with 1 ml normal saline 0.9% once/week 5 for weeks. Group 2 (50 nm GNPs): rats were i.m. injected with once/week with a dose of 75 µg 50 nm GNPs/kg bwt) for 5 weeks. In Group 3 (10 nm GNPs): male rats were i.m. injected with once/week with a dose of 75 µg 50 nm GNPs/kg bwt for 5 weeks, followed by 3 weeks washout period for all groups. Blood was collected at 3, 7, and 60 days in acute experiment, while, they were collected only before and after 2 months in chronic experiment. Acute and chronic administration of GNPs (10 or 50 nm size) in male albino rats induced no significant alterations for liver and kidney functions, lipid profile parameters and different haematological parameters at days 3 and 60 of the study. However, on day-7 post-treatment, GNPs-treated rats showed significantly (P <0.05) higher serum ALT, AST, ALP, urea, creatinine, glucose, and different lipid profile and decreased HDL level. Chronic administration of 10 nm or 50 nm GNPs significantly (P <0.05) decreased serum glucose levels. In conclusion acute or chronic administration of 10 nm or 50 nm GNPs could alter the liver, kidney functions and blood profile on day 7 post-treatment, however, these values returned to the normal levels on day 60 post- injection. Also, the chronic administration of GNPs induced a hypoglycemic effect in male albino rats