A system that learns to recognize 3-D objects

A system that learns to recognize 3-D objects from single and multiple views is presented. It consists of three parts: a simulator of 3-D figures, a Learner, and a recognizer. The 3-D figure simulator generates and plots line drawings of certain 3-D objects. A series of transformations leads to a number of 2-D images of a 3-D object, which are considered as different views and are the basic input to the next two parts. The learner works in three stages using the method of Learning from examples. In the first stage an elementary-concept learner learns the basic entities that make up a line drawing. In the second stage a multiple-view learner learns the definitions of 3-D objects that are to be recognized from multiple views. In the third stage a single-view learner learns how to recognize the same objects from single views. The recognizer is presented with line drawings representing 3-D scenes. A single-view recognizer segments the input into faces of possible 3-D objects, and attempts to match the segmented scene with a set of single-view definitions of 3-D objects. The result of the recognition may include several alternative answers, corresponding to different 3-D objects. A unique answer can be obtained by making assumptions about hidden elements (e. g. faces) of an object and using a multiple-view recognizer. Both single-view and multiple-view recognition are based on the structural relations of the elements that make up a 3-D object. Some analytical elements (e. g. angles) of the objects are also calculated, in order to determine point containment and conveziti. The system performs well on polyhedra with triangular and quadrilateral faces. A discussion of the system's performance and suggestions for further development is given at the end. The simulator and the part of the recognizer that makes the analytical calculations are written in C. The learner and the rest of the recognizer are written in PROLOG

Recognition of simple 3-D objects by the use of syntactic pattern recognition

This project is an attempt to supply the existing hardware with adequate software, in order to develop a system capable of recognizing 3-D objects, bounded by simple 2-D planes, which in turn are bounded by straight lines. [Continues.

Jouberin localizes to collecting ducts and interacts with nephrocystin-1

Joubert syndrome and related disorders are autosomal recessive multisystem diseases characterized by cerebellar vermis aplasia/hypoplasia, retinal degeneration and cystic kidney disease. There are five known genes; mutations of which give rise to a spectrum of renal cystic diseases the most common of which is nephronophthisis, a disorder characterized by early loss of urinary concentrating ability, renal fibrosis, corticomedullary cyst formation and renal failure. Many of the proteins encoded by these genes interact with one another and are located at adherens junctions or the primary cilia and or basal bodies. Here we characterize Jouberin, a multi-domain protein encoded by the AHI1 gene. Immunohistochemistry with a novel antibody showed that endogenous Jouberin is expressed in brain, kidney and HEK293 cells. In the kidney, Jouberin co-localized with aquaporin-2 in the collecting ducts. We show that Jouberin interacts with nephrocystin-1 as determined by yeast-2-hybrid system and this was confirmed by exogenous and endogenous co-immunoprecipitation in HEK293 cells. Jouberin is expressed at cell-cell junctions, primary cilia and basal body of mIMCD3 cells while a Jouberin-GFP construct localized to centrosomes in subconfluent and dividing MDCK cells. Our results suggest that Jouberin is a protein whose expression pattern supports both the adherens junction and the ciliary hypotheses for abnormalities leading to nephronophthisis

Superoxide dismutase downregulation in osteoarthritis progression and end-stage disease

Oxidative stress is proposed as an important factor in osteoarthritis (OA). To investigate the expression of the three superoxide dismutase (SOD) antioxidant enzymes in OA. SOD expression was determined by real-time PCR and immunohistochemistry using human femoral head cartilage. SOD2 expression in Dunkin–Hartley guinea pig knee articular cartilage was determined by immunohistochemistry. The DNA methylation status of the SOD2 promoter was determined using bisulphite sequencing. RNA interference was used to determine the consequence of SOD2 depletion on the levels of reactive oxygen species (ROS) using MitoSOX and collagenases, matrix metalloproteinase 1 (MMP-1) and MMP-13, gene expression. All three SOD were abundantly expressed in human cartilage but were markedly downregulated in end-stage OA cartilage, especially SOD2. In the Dunkin–Hartley guinea pig spontaneous OA model, SOD2 expression was decreased in the medial tibial condyle cartilage before, and after, the development of OA-like lesions. The SOD2 promoter had significant DNA methylation alterations in OA cartilage. Depletion of SOD2 in chondrocytes increased ROS but decreased collagenase expression. This is the first comprehensive expression profile of all SOD genes in cartilage and, importantly, using an animal model, it has been shown that a reduction in SOD2 is associated with the earliest stages of OA. A decrease in SOD2 was found to be associated with an increase in ROS but a reduction of collagenase gene expression, demonstrating the complexities of ROS function

Risk management of crude oil using refined products

SIGLEAvailable from British Library Document Supply Centre-DSC:DX190691 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

An Implicit Method for Interpolating Two Digital Closed Curves on Parallel Planes ⋆

Abstract. Ardon et al. [2] presented an implicit method for surface segmentation in 3D images. The boundary of the surface is assumed to be constrained by two given curves in the image. In this work we adopt the afore approach to interpolate two given digital curves lying on parallel planes, by introducing an artificial image potential, which is based on a triangular facet surface interpolation technique.

Metabolism of α‐Glyceryl Ethers by Crithidia fasciculata. I. Study of the In Vivo Degradation of Exogenous Chimyl and Batyl Alcohols

ABSTRACT. [14C]chimyl and [3H]batyl alcohols were added to Crithidia fasciculata cultures during the mid‐log phase of cell growth, and the lipid extracts of the cells were analyzed for degradation products. C. fasciculata cells were able to take up exogenous glyceryl ethers, and in amounts as high as the endogenous lipid content. The glyceryl ether taken up by the cells was incorporated into lipids either prior to the ether bond cleavage or after degradation to fatty acid. The extent of degradation and the degree of incorporation of degradation products into cellular lipid were higher for chimyl than for batyl alcohol. Batyl alcohol was not metabolized efficiently, leading to the formation of large intracellular pools of free substrate. One product of glyceryl ether degradation was identified as alkyl‐dihydroxy acetone, and was detected inside and outside of the cells. The data strongly suggest that this product is the first stable intermediate in the degradation process and indicate that the extracellular formation of alkyl‐dihydroxy acetone is due to the action of exocnzyme; ecteted by the cells. The constant detection of alk I cnyl glycerol among the degradation products indicates the existence of a second mechantsm in C. fasciculata for converting the alkyl‐to alkenyl‐glycerol. Copyright © 1981, Wiley Blackwell. All rights reserve