Glaucoma related proteomic alterations in human retina samples

Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma

Vulnerability and vulnerable groups from an intersectionality perspective

In general, the identification and protection of vulnerable groups in the case of hazards or when a crisis unfolds is an issue that any crisis and disaster risk management should address, since people have different levels of exposure to hazards and crises. In this article, we promote the application of the intersectionality perspective in the study of vulnerable groups, and we call for intersectionality as a guiding principle in risk and crisis management, to provide a better and more nuanced picture of vulnerabilities and vulnerable groups. This can help national and local authorities and agencies to formulate specific guides, to hire staff with the skills necessary to meet particular needs, and to inform vulnerable groups in a particular way, taking into account the differences that may coexist within the same group. Intersectionality allows us to read vulnerability not as the characteristic of some socio-demographic groups. It is rather the result of different and interdependent societal stratification processes that result in multiple dimensions of marginalisation. In this vein, we argue that research should focus on 1) self-perceived vulnerability of individuals and an intersectionality approach to unpack vulnerable groups; 2) cases of crises according to the level and/or likelihood of individual exposure to hazards, to better nuance issues of vulnerability.publishedVersio

Communication-related vulnerability to disasters: A heuristic framework

The concept of social vulnerability has been increasingly applied in disaster literature, but its communicative drivers have remained understudied. In this article, we put forward a heuristic framework for explaining how communication-related factors may adversely affect people's capacity to prepare for and respond to disasters. This will help researchers, policy makers, and practitioners in the field of disasters and crises to systematically identify individual, social-structural, and situational factors of vulnerability that shape how people access, understand, and act upon information about hazards. We integrate ideas from recent literature on information disorders – various forms and effects of false or harmful information that are characteristic to modern communication ecosystems – to improve our understanding of how the new media environments may transform the ways people learn about hazards and cope with disasters.publishedVersio

Replication Data for: Research Methodology and Community Participation

The data and supplemental appendix describing the data collection and coding process for the article "Research Methodology and Community Participation: A Decade of Indigenous Social Science Research in Canada," to be published in Canadian Review of Sociology/Revue canadienne de sociologie, 57, 1 (Feburary), 2020. This research was supported by a Knowledge Synthesis Grant (872-2016-0013) from the Social Sciences and Humanities Research Council of Canada. Research assistance was provided by Ben Manshanden and Marrissa Mathews

Glaucoma related proteomic alterations in human retina samples

Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma

Development, piloting and evaluation of an app-supported psychosocial prevention intervention to strengthen participation in working life: a study protocol of a mixed-methods approach

Introduction Rates of incapacity to work due to mental disorders have increased in many European countries. The consequences of persistent stress can impact individuals’ physical and psychological well-being and gradually develop into chronic stress. Mental disorders or symptoms of burn-out syndrome can have severe consequences. Mental disorders leading to work incapacity significantly burden the health system. Prevention interventions can protect against burn-out, depression, anxiety and other mental health disorders. Digital health is a promising approach to increase the utilisation of effective prevention interventions. This mixed-methods study evaluates a newly developed app-supported psychosocial prevention intervention called ‘RV Fit Mental Health’ to strengthen participation in working life.Methods and analysis The study uses a three-stage parallel mixed-methods design. This study accompanies the development (stage 1), piloting (stage 2) and evaluation (stage 3) of the new intervention. Within the stages, there is a quantitative as well as a qualitative research strand. Employed persons with an incipient mental disorder will be included. Additionally, experts within the project or connected areas will be included. Quantitative data will be analysed using multifactorial variance analyses in a pre–post design. Qualitative data will be analysed using qualitative content analysis. The study is a comprehensive research approach to investigate the development, piloting and evaluation of an app-supported psychosocial app-based prevention intervention. The rigour of the study will be achieved through data triangulation.Ethics and dissemination All participants will receive detailed study information and give written informed consent before data collection. Ethical approval was obtained from the Technical University of Munich Ethics Committee. All data collection will follow all legislative rules regarding data protection, also following the Declaration of Helsinki. The study results will be disseminated in peer-reviewed journals and presented at international conferences.Trial registration numbers DRKS00030818 and DRKS00033080