31 research outputs found
DMTs and Covid-19 severity in MS: a pooled analysis from Italy and France
We evaluated the effect of DMTs on Covid-19 severity in patients with MS, with a pooled-analysis of two large cohorts from Italy and France. The association of baseline characteristics and DMTs with Covid-19 severity was assessed by multivariate ordinal-logistic models and pooled by a fixed-effect meta-analysis. 1066 patients with MS from Italy and 721 from France were included. In the multivariate model, anti-CD20 therapies were significantly associated (OR = 2.05, 95%CI = 1.39â3.02, p < 0.001) with Covid-19 severity, whereas interferon indicated a decreased risk (OR = 0.42, 95%CI = 0.18â0.99, p = 0.047). This pooled-analysis confirms an increased risk of severe Covid-19 in patients on anti-CD20 therapies and supports the protective role of interferon
Optical Properties of Natural Organic Matter in the Yukon River Basin (Extended Abstract)
Variability in dissolved organic matter optical properties in surface waters in the Amerasian Basin
Thulium spiked gel for internal standardisation in LA-ICP-MS bio-imaging: quantitative elemental distribution of uranium in kidney tissue
International audienceThe quantitative analysis of trace metals in different organs or cellular structures is a topic of emerging interest for the assessment of toxicological risk. The kidney is recognized as a major site for uranium accumulation able to induce renal toxicity. Due to its heterogeneous distribution within the tissue, the quantification appears to be of significant interest. Mass spectrometry imaging (MSI) using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been employed for mapping and quantifying uranium in histological tissue sections of mouse kidney. Samples were immediately immersed into a PFA solution during 24h, OCT embedded and stored at -80°C until cutting in cryostat and mounting in gel covered glass slides. Sample preparation (tissue section thickness) and laser conditions were carefully optimized to assure the complete ablation of the sample matrix. This works presents the development of internal standard correction methodology based on doped gelatine. In order to correct matrix effects, lack of tissue homogeneity and instrumental drift, a thulium (Tm, internal standard) spiked 10% (m/v) gel was prepared and deposited on the top of glass microscope slides. The reproducibility and homogeneity of the deposited gel was assessed by ablating several areas (0.5 x 0.5 mm, n=20) in which Tm was recorded. For quantification purposes, matrix-matched laboratory standards were prepared from a pool of mouse kidneys by dosing each level with different concentration of uranium. In the same way, several quality control (QC) samples were synthetized to prove the accuracy and precision of the analytical method. The unspiked tissue was used for blank control. The concentrations were verified by conventional liquid ICP-MS after acid digestion. These standards were cut and mounted onto glass slides in the same way as the samples.Thulium spiked gel was demonstrated to be adequate for compensation of instrumental drifts during measuring time and matrix effects, which improves the quantification of elemental distributions and allows a trustworthy comparison of experiments performed at different times. The proposed analytical bio-imaging approach was successfully applied for quantification of uranium of kidney samples. The comparison of the calculated average concentration obtained by LA-ICP-MS and the results obtained after liquid ICP-MS analysis were in good accordance
The nature of colored dissolved organic matter in the southern Canada Basin and East Siberian Sea
http://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr08-04/
Applications de lâimagerie SIMSau sein de la plateforme PATERSON de lâIRSN
International audienceLa microscopie ionique analytique SIMS, permet lâanalyse Ă©lĂ©mentaire et isotopique dâune surface solide par faisceau dâions couplĂ©e Ă un spectromĂštre de masse. Le principe consiste Ă bombarder lâĂ©chantillon Ă Ă©tudier Ă lâaide dâun faisceau de particules chargĂ©es (dizaine de kV). Ce faisceau dâions primaires rentre en collision avec lâĂ©chantillon cible provoquant lâĂ©jection de particules provenant des couches superficielles de lâĂ©chantillon. Une fraction de ces particules est spontanĂ©ment ionisĂ©e. Ces ions dit secondaires, sont ensuite accĂ©lĂ©rĂ©s et dirigĂ©s Ă lâentrĂ©e dâun spectromĂštre de masse. Lâanalyse de ces ions secondaires permet ainsi de caractĂ©riser et dâimager la composition chimique Ă©lĂ©mentaire et isotopique de la surface de lâĂ©chantillon.Cette technique est dâun apport essentiel dans le domaine du nuclĂ©aire notamment pour lâĂ©tude de la distribution dâĂ©lĂ©ments stables ou de radio-isotopes aussi bien dans des matrices biologiques par des Ă©tudes de micro localisation de lâuranium dans des tissus rĂ©naux contaminĂ©s que gĂ©ologiques avec mise en Ă©vidence de la signature radiogĂ©nique en plomb de phase uranifĂšre issue de rĂ©sidu de traitement de minerai dâUranium . Ainsi lâĂ©quipement SIMS 4FE7 implantĂ© au sein de la plateforme PATERSON de lâIRSN est utilisĂ© dans le domaine de la radioprotection pour amĂ©liorer la comprĂ©hension des mĂ©canismes de transport ainsi que dâaccumulations des radionuclĂ©ides. Dans le domaine de la sĂ»retĂ© nuclĂ©aire, il permet Ă©galement dâaider Ă la comprĂ©hension de la tenue des gaines de combustible Ă lâirradiation en cartographiant les Ă©lĂ©ments hydrogĂšne et oxygĂšne
Applications de lâimagerie SIMSau sein de la plateforme PATERSON de lâIRSN
International audienceLa microscopie ionique analytique SIMS, permet lâanalyse Ă©lĂ©mentaire et isotopique dâune surface solide par faisceau dâions couplĂ©e Ă un spectromĂštre de masse. Le principe consiste Ă bombarder lâĂ©chantillon Ă Ă©tudier Ă lâaide dâun faisceau de particules chargĂ©es (dizaine de kV). Ce faisceau dâions primaires rentre en collision avec lâĂ©chantillon cible provoquant lâĂ©jection de particules provenant des couches superficielles de lâĂ©chantillon. Une fraction de ces particules est spontanĂ©ment ionisĂ©e. Ces ions dit secondaires, sont ensuite accĂ©lĂ©rĂ©s et dirigĂ©s Ă lâentrĂ©e dâun spectromĂštre de masse. Lâanalyse de ces ions secondaires permet ainsi de caractĂ©riser et dâimager la composition chimique Ă©lĂ©mentaire et isotopique de la surface de lâĂ©chantillon.Cette technique est dâun apport essentiel dans le domaine du nuclĂ©aire notamment pour lâĂ©tude de la distribution dâĂ©lĂ©ments stables ou de radio-isotopes aussi bien dans des matrices biologiques par des Ă©tudes de micro localisation de lâuranium dans des tissus rĂ©naux contaminĂ©s que gĂ©ologiques avec mise en Ă©vidence de la signature radiogĂ©nique en plomb de phase uranifĂšre issue de rĂ©sidu de traitement de minerai dâUranium . Ainsi lâĂ©quipement SIMS 4FE7 implantĂ© au sein de la plateforme PATERSON de lâIRSN est utilisĂ© dans le domaine de la radioprotection pour amĂ©liorer la comprĂ©hension des mĂ©canismes de transport ainsi que dâaccumulations des radionuclĂ©ides. Dans le domaine de la sĂ»retĂ© nuclĂ©aire, il permet Ă©galement dâaider Ă la comprĂ©hension de la tenue des gaines de combustible Ă lâirradiation en cartographiant les Ă©lĂ©ments hydrogĂšne et oxygĂšne
Horsesâ Tactile Reactivity Differs According to the Type of Work: The Example of Equine-Assisted Intervention
International audienceTactile perception in humans varies between individuals and could depend on extrinsic factors such as working activity. In animals, there is no study relating the influence of animalsâ work and their tactile reactivity per se. We investigated horsesâ tactile reactivity using von Frey filament in different body areas and compared horses working only in equine-assisted interventions (EAI), in riding school (RS) lessons, and in both activities (EAI-RS). We further compared tactile actions by people with or without mental and/or developmental disorders during brushing sessions. The results indicated that EAI horses showed higher tactile reactivity compared to EAI-RS and RS horses, both in terms of number of reactions overall, and especially when the test involved thin filaments. All horses showed high tactile reactivity when tested on the stifle, and this was particularly true for EAI horses. These differences could be related to humansâ actions, as participants diagnosed with disorders brushed more the hindquarters and showed more fragmented actions. This study opens new lines of thought on the influence of EAI working activity on horsesâ tactile reactivity, and hence, on horsesâ sensory perception. Tactile reactivity outside work, may be directly (via tactile stimulations) or indirectly (via the welfare state), influenced by working conditions