Occurrence Of Low Molecular Weight Phenolics In Vitis Vinifera Red Grape Cultivars And Their Winemaking By-products From São Paulo (brazil)

The content of low molecular weight phenolic compounds present in winemaking by-products (skins from grape marc and wine lees) of red wine made from the Vitis vinifera grape cultivars Cabernet Sauvignon and Cabernet Franc in the state of São Paulo (Brazil) has been examined. The study was extended to two consecutive years and showed that winemaking by-products still contained high concentrations of interesting functional and bioactive phenolic compounds. In addition to original grape phenolics, the winemaking by-products contained new phenolics originated by the action of enzymatic (yeast mediated) and chemical reactions during winemaking, including formation of anthocyanin-derived pigments and hydrolysis products from flavonol glycosides and tartaric esters of hydroxycinnamic acids. A total of 19 anthocyanins, 9 pyranoanthocyanins, 18 flavonols, 7 hydroxycinnamic acid derivatives, and 3 resveratrol-based stilbenes were identified. Wine lees have been revealed as an interesting source of flavonol aglycones and pyranoanthocyanins. The best sample preparation technique prior to the extraction of phenolic compounds, with regard to the maintenance of phenolic compound profiles and concentrations, was freeze-drying. However, in the case of lees, spray-drying showed to be also effective in the preservation of phenolic compounds and is a less expensive technique. Oven-drying at 50. °C should be avoided if there is interest in using winemaking by-products for further purposes, since phenolic compound concentrations were strongly reduced by thermal degradation. © 2014 Elsevier Ltd.62500513Amico, V., Napoli, E.M., Renda, A., Ruberto, G., Spatafora, C., Tringali, C., Constituents of grape pomace from the Sicilian cultivar 'Nerello Mascalese' (2004) Food Chemistry, 88, pp. 599-607Babbar, N., Oberoi, H.S., Uppal, D.S., Patil, R.T., Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues (2011) Food Research International, 44, pp. 391-396Blanco-Vega, D., López-Bellido, F.J., Alía-Robledo, J.M., Hermosín-Gutiérrez, I., HPLC-DAD-ESI-MS/MS characterization of pyranoanthocyanins pigments formed in model wine (2011) Journal of Agricultural and Food Chemistry, 59, pp. 9523-9531Boulton, R., The copigmentation of anthocyanins and its role in the color of red wine: A critical review (2001) American Journal of Enology and Viticulture, 52, pp. 67-87Castillo-Muñoz, N., Fernández-Gonzalez, M., Gómez-Alonso, S., García-Romero, E., Hermosín-Gutiérrez, I., Red-color related phenolic composition of Garnacha Tintorera (Vitis vinifera L.) grapes and red wines (2009) Journal of Agricultural and Food Chemistry, 57, pp. 7883-7891Castillo-Muñoz, N., Gómez-Alonso, S., García-Romero, E., Hermosín-Gutiérrez, I., Flavonol profiles of Vitis vinifera red grapes and their single-cultivar wines (2007) Journal of Agricultural and Food Chemistry, 55, pp. 992-1002Cheng, V.J., Bekhit, A.E.A., McConnell, M., Mros, S., Zhao, J., Effect of extraction solvent, waste fraction and grape variety on the antimicrobial and antioxidant activities of extracts from wine residue from cool climate (2012) Food Chemistry, 134, pp. 474-482Cortés, S., Rodríguez, R., Salgado, J.M., Domínguez, J.M., Comparative study between Italian and Spanish grape marc spirits in terms of major volatile compounds (2011) Food Control, 22, pp. 673-680Ferrandino, A., Carra, A., Rolle, L., Schneider, A., Schubert, A., Profiling of hydroxycinnamoyl tartrates and acylated anthocyanins in the skin of 34 Vitis vinifera genotypes (2012) Journal of Agricultural and Food Chemistry, 60, pp. 4931-4945Hermosín-Gutiérrez, I., Castillo-Muñoz, N., Gómez-Alonso, S., García-Romero, E., Flavonol profiles for grape and wine authentication (2011) Progress in authentication of food and wine, pp. 113-129. , American Chemical Society, ACS Symposium Series, Washington, DC, S.E. Ebeler, G.R. Takeoka, P. Winterhalter (Eds.)Kammerer, D., Claus, A., Carle, R., Schieber, A., Polyphenol screening of pomace from red and white grape varieties (Vitis vinifera L.) by HPLC-DAD-MS/MS (2004) Journal of Agricultural and Food Chemistry, 52, pp. 4360-4367Kammerer, D., Claus, A., Schieber, A., Reinhold, C., A novel process for the recovery of polyphenols from grape (Vitis vinifera L.) pomace (2005) Journal of Food Science, 70 (2), pp. 157-163Koundouras, S., Hatzidimitriou, E., Karamolegkou, M., Dimopoulou, E., Kallithraka, S., Tsialtas, J.T., Irrigation and root stock effects on the phenolic concentration and aroma potential of Vitis vinifera L. cv. Cabernet Sauvignon grapes (2009) Journal of Agricultural and Food Chemistry, 57, pp. 7805-7813Lago-Vanzela, E.S., Da-Silva, R., Gomes, E., García-Romero, E., Hermosín-Gutiérrez, I., Phenolic composition of the edible parts (flesh and skin) of BordÔ grape (Vitis labrusca) using HPLC-DAD-ESI-MS/MS (2011) Journal of Agricultural and Food Chemistry, 59, pp. 13136-13146Lago-Vanzela, E.S., Da-Silva, R., Gomes, E., García-Romero, E., Hermosín-Gutiérrez, I., Phenolic composition of the Brazilian seedless table grape varieties BRS Clara and BRS Morena (2011) Journal of Agricultural and Food Chemistry, 59, pp. 8314-8323Maragkoudakis, P.A., Nardi, T., Bovo, B., D'Andrea, M., Howell, K.S., Giacomini, A., Biodiversity, dynamics and ecology of bacterial community during grape marc storage for the production of grappa (2013) International Journal of Food Microbiology, 162 (2), pp. 143-151Mendes, J.A.S., Prozil, S.O., Evtuguin, D.V., Lopes, L.P.C., Towards comprehensive utilization of winemaking residues: Characterization of grape skins from red grape pomaces of variety Touriga Nacional (2013) Industrial Crops and Products, 43, pp. 25-32Morata, A., Gómez-Cordovés, M.C., Colomo, B., Suárez, J.A., Cell Wall anthocyanin adsorption by different Saccharomyces strains during the fermentation of Vitis vinifera L. cv Graciano grapes (2005) European Food Research and Technology, 220, pp. 341-346Nixdorf, S.L., Hermosín-Gutiérrez, I., Brazilian red wines made from the hybrid grape cultivar Isabel: Phenolic composition and antioxidant capacity (2010) Analytica Chimica Acta, 659, pp. 208-215Paradelo, R., Moldes, A.B., Barral, M.T., Utilization of a factorial design to study the composting of hydrolyzed grape marc and vinification lees (2010) Journal of Agricultural and Food Chemistry, 58, pp. 3085-3092Poudel, P.R., Mochioka, R., Beppu, K., Kataoka, I., Influence of temperature on berry composition of interspecific hybrid wine grape 'Kadainou R-1' (Vitis ficifolia var. ganebu×V. vinifera 'Muscat of Alexandria') (2009) Journal of the Japanese Society for Horticultural Science, 78, pp. 169-174Rebello, L.P.G., Lago-Vanzela, E.S., Barcia, M.T., Ramos, A.M., Stringheta, P.C., Da-Silva, R., Phenolic composition of the berry parts of hybrid grape cultivar BRS Violeta (BRS Rubea×IAC 1398-21) using HPLC-DAD-ESI-MS/MS (2013) Food Research International, 54, pp. 354-366Rockenbach, I.I., Gonzaga, L.V., Rizelio, V.M., Gonçalves, A.E.S.S., Genovese, M.I., Fett, R., Phenolic compounds and antioxidant activity of seed and skin extracts of red grape (Vitis vinifera and Vitis labrusca) pomace from Brazilian winemaking (2011) Food Research International, 44, pp. 897-901Rubilar, M., Pinelo, M., Shene, C., Sineiro, J., Nuñez, M.J., Separation and HPLC-MS identification of phenolic antioxidants from agricultural residues: Almond hulls and grape pomace (2007) Journal of Agricultural and Food Chemistry, 55, pp. 10101-10109Ryan, J., Revilla, E., Anthocyanin composition of Cabernet Sauvignon and Tempranillo grapes at different stages of ripening (2003) Journal of Agricultural and Food Chemistry, 51, pp. 3372-3378Shrikhande, A.J., Wine by-products with health benefits (2000) Food Research International, 33, pp. 469-474Slier, E.O., Neira, A.P., Solís, R.L., Marín, F.Z., Da-Silva, J.M.R., Laureano, O., Comparative study of the phenolic composition of seeds and skins from Carménère and Cabernet Sauvignon grape varieties (Vitis vinifera L.) during ripening (2010) Journal of Agricultural and Food Chemistry, 58, pp. 3591-359

Performance during a strenuous swimming session is associated with high blood lactate: pyruvate ratio and hypoglycemia in fasted rats

<div><p>The aim of this study was to investigate the effect of lactatemia elevation and glycemia reduction on strenuous swimming performance in fasted rats. Three rats were placed in a swimming tank at the same time. The first rat was removed immediately (control group) and the remaining ones were submitted to a strenuous swimming session. After the second rat was exhausted (Exh group), the third one was immediately removed from the water (Exe group). According to the period of time required for exhaustion, the rats were divided into four groups: low performance (3–7 min), low-intermediary performance (8–12 min), high-intermediary performance (13–17 min), and high performance (18–22 min). All rats were removed from the swimming tanks and immediately killed by decapitation for blood collection or anesthetized for liver perfusion experiments. Blood glucose, lactate, and pyruvate concentrations, blood lactate/pyruvate ratio, and liver lactate uptake and its conversion to glucose were evaluated. Exhaustion in low and low-intermediary performance were better associated with higher lactate/pyruvate ratio. On the other hand, exhaustion in high-intermediary and high performance was better associated with hypoglycemia. Lactate uptake and glucose production from lactate in livers from the Exe and Exh groups were maintained. We concluded that there is a time sequence in the participation of lactate/pyruvate ratio and hypoglycemia in performance during an acute strenuous swimming section in fasted rats. The liver had an important participation in preventing hyperlactatemia and hypoglycemia during swimming through lactate uptake and its conversion to glucose.</p></div

Lodo de esgoto e sistema radicular da pupunheira Sewage sludge doses and the root system of peach palm

Os efeitos do lodo de esgoto sobre o sistema radicular da pupunheira foram estudados em experimento em campo, em blocos casualizados, instalado em Ubatuba (SP), em julho 2001, na densidade de 5.000 plantas ha-1. As doses de lodo de esgoto fresco (79,7 % de umidade) utilizadas foram de 0, 38, 76 e 152 t ha-1, equivalentes a 0, 100, 200 e 400 kg ha-1 de N. Os tratamentos foram aplicados no sulco de plantio, adicionando-se 15 g por planta de KCl como fonte de K. A análise do sistema radicular foi efetuada doze meses após, por meio de trado e de trincheiras e com o auxílio de fotos digitais. Detectou-se que o sistema radicular de pupunheiras com um ano de campo estava concentrado nas camadas superficiais (acima de 75 % nos primeiros 20 cm), assim como em distâncias de até 0,5 m da base da planta. O lodo de esgoto modificou positivamente a densidade do solo, alterando também favoravelmente a densidade das raízes. Houve aumento na biomassa radicular proporcional às doses de lodo de esgoto, existindo uma relação direta daquela com a fitomassa aérea. Doses de lodo equivalentes a 200 e 400 kg de N contribuíram para aprofundar o sistema radicular e proporcionaram maior quantidade relativa de raízes finas.<br>The effects of sewage sludge doses on the root system of peach palm were studied in a field experiment, in randomized complete blocks, carried out in Ubatuba, state of São Paulo, Brazil. The trial was set up in July 2001, with a density of 5,000 plants ha-1. The sewage sludge doses (79.7 % water content) were 0, 38, 76, and 152 t ha-1, which were equivalent to doses of 0, 100, 200, and 400 kg ha-1 of N. The treatments were applied in the planting furrow, and every plant was provided with K by a dose of 15 g potassium chloride. The root system was analyzed one year after planting using soil auger and digging of trenches and with the help of digital images. It was concluded that the root system of one-year old peach palm was concentrated in the upper soil layer (over 75 % in the 0-20 cm surface layer), and within a horizontal distance of 0.50 m from the palm trunk. The sewage sludge positively modified the soil density as well as the root density. There was an increment in root biomass proportional to the sludge doses, and there was a positive linear relationship between the root and shoot biomass. Sludge doses equivalent to 200 and 400 kg ha-1 of N allowed the root system to explore deeper soil layers, as well as the development of more fine roots compared to the other treatments

Un anno di "International Journal of Industrial Organization"

Embora a aveia preta apresente importantes características de interesse agronômico, os estudos direcionados ao conhecimento da planta visando aumentos de produtividade são ainda em pequeno número em condições brasileiras. Este trabalho teve por objetivo avaliar o desenvolvimento de plantas de aveia preta, cultivar Comum. O experimento foi instalado no mês de maio, em condições de campo, em Nitossolo Vermelho, em Botucatu, SP. O delineamento experimental empregado foi inteiramente casualizado, com três repetições. Os tratamentos constaram de épocas de coletas de plantas, as quais foram iniciadas aos 21 dias após a emergência das plântulas (DAE). O maior desenvolvimento vegetativo das plantas, considerando-se o número total de perfilhos e o número total de folhas, foi observado na fase de emissão da panícula, aos 84 DAE. A contribuição dos perfilhos primários foi maior que a dos perfilhos secundários, tanto nos componentes vegetativos como nos componentes relacionados à produção de sementes

Respostas de crescimento da pupunheira à adubação NPK Peach palm growth responses to NPK fertilization

As palmeiras apresentam grande demanda por nutrientes. Por este motivo, o efeito da adubação NPK no crescimento de pupunheiras (Bactris gasipaes Kunth) foi estudado durante 30 meses (11/90 a 04/93) em experimento conduzido a campo em solo Aluvial álico (corrigido por meio de calagem) em Ubatuba, SP (clima "Cfa"). Utilizou-se o delineamento de blocos ao acaso, com uma repetição, em esquema fatorial fracionado (½ de 4³), com tratamentos dispostos em dois blocos, parcelas úteis de 24 plantas e bordaduras duplas ao redor. Foram testadas quatro doses de nitrogênio (0, 100, 200 e 400 kg ha-1 ano-1 de N), fósforo (0, 50, 100 e 200 kg ha-1 ano-1 de P2O5) e potássio (0, 50, 100 e 200 kg ha-1 ano-1 de K2O), aplicadas em faixa e divididas em cinco aplicações anuais, utilizando como fontes nitrocálcio, superfosfato triplo e cloreto de potássio. As plantas foram cultivadas no espaçamento de 2 × 1 m, e avaliadas periodicamente quanto ao diâmetro da haste principal, número de perfilhos e porcentagem de plantas perfilhadas. Em solo arenoso e de baixa fertilidade, a pupunheira apresenta resposta linear, positiva e significativa de crescimento às adubações com nitrogênio (N) e potássio (K) e ausência de resposta ao fósforo (P). As primeiras respostas significativas ocorreram três meses depois de iniciadas as adubações diferenciadas. Não houve interações significativas entre N, P e K. O crescimento máximo foi obtido com doses anuais de 400 kg de N, 0 kg de P2O5 e 200 kg de K2O por hectare.<br>Palms have high nutrient demand. For this reason, the effects of NPK fertilization on the initial growth of peach palm (Bactris gasipaes Kunth) were studied in a field experiment, from November 1990 to April 1993. The trial was set up on an allic, previously limed soil, in Ubatuba, SP, Brazil (23º27'S, 45º04'W, 6 m asl; "Cfa" climate). A fractional factorial experimental design (½ of 4³) was utilized, with two blocks, one replication, 24 inner plants per plot and double border rows. Treatments were composed of four rates of nitrogen (0 to 400 kg N ha-1 year-1), phosphorus (0 to 200 kg P2O5 ha-1 year-1) and potassium (0 to 200 kg K2O ha-1 year-1), applied in stripes and divided in five annual applications. The fertilizers used were: calcium nitrate, triple super phosphate and potassium chloride. Plants were cultivated in a 2 × 1 m array, and periodically evaluated in relation to diameter of main stem, number of offshoots and percentage of plants with offshoots. In this sandy and low fertility soil, peach palm growth responses in relation to N and K amendments were linear and positive. Initial responses were observed three months after fertilizer application. No isolated effects of P fertilization, nor significant interactions among fertilizers were observed. Maximum growth responses were reached with 400 kg N ha-1 year-1, 0 kg P2O5 ha-1 year-1 and 200 kg K2O ha-1 year-1