17 research outputs found
The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells
MR elastography, T1 and T2 relaxometry of liver: role in noninvasive assessment of liver function and portal hypertension
Magnetic resonance elastography to quantify liver disease severity in autosomal recessive polycystic kidney disease
Assessing spleen stiffness by point shear‐wave elastography: Is it feasible and reproducible in patients with chronic liver disease? Is it useful to predict portal hypertension?
Oxidative status of growing West African dwarf goat kids fed diets containing different nutrient density
Simultaneous measurement of hepatic and splenic stiffness using MR elastography: preliminary experience
Spleen Dynamic Contrast-Enhanced Magnetic Resonance Imaging as a New Method for Staging Liver Fibrosis in a Piglet Model
Deconvoluting hepatic processing of carbon nanotubes
Single-wall carbon nanotubes present unique opportunities for drug delivery, but have not advanced into the clinic. Differential nanotube accretion and clearance from critical organs have been observed, but the mechanism not fully elucidated. The liver has a complex cellular composition that regulates a range of metabolic functions and coincidently accumulates most particulate drugs. Here we provide the unexpected details of hepatic processing of covalently functionalized nanotubes including receptor-mediated endocytosis, cellular trafficking and biliary elimination. Ammonium-functionalized fibrillar nanocarbon is found to preferentially localize in the fenestrated sinusoidal endothelium of the liver but not resident macrophages. Stabilin receptors mediate the endocytic clearance of nanotubes. Biocompatibility is evidenced by the absence of cell death and no immune cell infiltration. Towards clinical application of this platform, nanotubes were evaluated for the first time in non-human primates. The pharmacologic profile in cynomolgus monkeys is equivalent to what was reported in mice and suggests that nanotubes should behave similarly in humans