20 research outputs found
Análisis de la expresión de genes de respuesta inmune durante el desarrollo ontogénico de paralarvas de pulpo Octopus vulgaris criadas en cautividad
El pulpo común es una especie de gran importancia comercial, considerada como una especie
emergente en acuicultura. En este trabajo se analizó el nivel de expresión de los genes inmunes
TLR, C1q, Galectina, PGRP, LITAF, SERPIN, PRDX y Caspasa 3 mediante PCR cuantitativa (q-PCR)
en embriones y paralarvas de O. vulgaris de edades 0, 10, 20 y 34 días. Adicionalmente, se
infectaron paralarvas de 22 días con bacterias patógenas vivas Vibrio lentus y V. splendidus a 1h,
4h y 24h. El estudio del desarrollo del sistema inmune de estas paralarvas ayudará a identificar
factores claves para la supervivencia y cultivo del pulpo común. Durante el desarrollo ontogénico,
los embriones mostraron el menor nivel de expresión de PGRP, Caspasa 3 y PRDX. Por el contrario,
C1q, Galectina y LITAF se observaron visiblemente expresados. C1q, TLR y SERPIN fueron los
genes que presentaron mayor nivel de expresión en Pa0D. A partir de Pa10D se observó un
notable incremento en la expresión de C1q, Galectina, PGRP y LITAF. La expresión de Caspasa3 se
incrementó gradualmente desde Em. V. lentus y V. splendidus inducen un notable incremento de la
expresión de C1q y PRDX entre 1h y 4h post infección. Sin embargo, durante las primeras horas de
infección se observó una disminución de la expresión de Galectina, TLR, PGRP y LITAF.
Particularmente la infección por V. lentus produjo una disminución de la expresión de SERPIN. Los
resultados obtenidos sugieren que la capacidad del sistema inmune de reconocer patógenos y
evitar infecciones es significativamente activo desde los estadíos de paralarvas recién eclosionadas.
Sin embargo, se observó un aumento significativo de los genes seleccionados a partir de Pa10D.The common octopus is a species of high commercial interest and nowadays is considered as an
emergent species in aquaculture. The mRNA expression level of immune-related genes (TLR, C1q,
Galectin, PGRP, LITAF, SERPIN, PRDX and Caspase 3) was analyzed by Real time qPCR on
embryos and paralarvae of O. vulgaris at age of 0, 10, 20 and 34 days. Additionally, paralarvae of
22 days were challenged with live Vibrio lentus and V. splendidus during a time course (1 h, 4 h
and 24 h). This analysis will allow a better understanding of the developmental of immune system
of these paralarvae, which will help to identify key factors for survival and culture of the common
octopus. Regarding ontogeny, Em showed the lowest expression of PGRP, Caspase 3 and PRDX. In
contrast, C1q, Galectin and LITAF were visibly expressed. C1q, TLR and SERPIN were highly
expressed in Pa0D. A notably increase in the expression of C1q, Galectin, PGRP and LITAF began
from Pa10D. Caspase 3 expression was gradually increased from Em. V. lentus and V. splendidus
induced a notable expression of C1q and PRDX at 1 h and 4 h post infection (p. i.). Nevertheless,
they markedly suppress the activation of Galectin, TLR, PGRP and LITAF during the first hours p.i..
Particularly, V. lentus suppressed the expression of SERPIN. The present results suggest that the
ability of immune system to recognize pathogens and avoid infections is a priority in recently
hatched paralarvae. However, a noticeable increase of gene expression was observed from Pa10D.0,000
RELATIONSHIP BETWEEN ZOOPLANKTONICS SPECIES, PHYSICS PARAMETERS AND AGE IN WILD PARALARVAE OF Octopus vulgaris
Association of Systemic Lupus Erythematosus Clinical Features with European Population Genetic Substructure
Systemic Lupus Erythematosus (SLE) is an autoimmune disease with a very varied spectrum of clinical manifestations that could be partly determined by genetic factors. We aimed to determine the relationship between prevalence of 11 clinical features and age of disease onset with European population genetic substructure. Data from 1413 patients of European ancestry recruited in nine countries was tested for association with genotypes of top ancestry informative markers. This analysis was done with logistic regression between phenotypes and genotypes or principal components extracted from them. We used a genetic additive model and adjusted for gender and disease duration. Three clinical features showed association with ancestry informative markers: autoantibody production defined as immunologic disorder (P = 6.8×10(-4)), oral ulcers (P = 6.9×10(-4)) and photosensitivity (P = 0.002). Immunologic disorder was associated with genotypes more common in Southern European ancestries, whereas the opposite trend was observed for photosensitivity. Oral ulcers were specifically more common in patients of Spanish and Portuguese self-reported ancestry. These results should be taken into account in future research and suggest new hypotheses and possible underlying mechanisms to be investigated. A first hypothesis linking photosensitivity with variation in skin pigmentation is suggested
Selection of reliable reference genes for RT-qPCR studies in Octopus vulgaris paralarvae during development and immune-stimulation
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