Almansa, E. (Eduardo)

Bartolomé-Baraza, A. (Aurora)

García-Fernández, Pablo

Gestal, Camino

Nande, M. (Manuel)

Otero-Pinzas, J.J. (Juan José)

Perales-Raya, C. (Catalina)

English

Gestal, C. (Camino)

García-Fernández, P. (Pablo)

Repositorio Institucional Digital del IEO

AGEING EARLY LIFE STAGES OF Octopus vulgarisEFFECT OF TEMPERATUREC. Perales-Raya,  M. Nande, A. Bartolomé,  C. Gestal, JJ. Otero, P. García-Fernández & E. AlmansaWild and cultured populations of common octopus (Octopus vulgaris)have a high economic value worldwideBackground - AIMSWe planned a comparative analysis of wild and cultured paralarvae of similar age (gene expression, physiology, biochemistry, larval behavior, ecology) Aim – Age estimation of wild paralarvae in O. vulgarisSurvival of the species during its planktonic early life: critical for the population success. Still low survival in captivityUnderstanding early stages in the wild and the optimization of culture conditions37 paralarvae (0-22 days) reared at 14ºCSimilar temperature than wild samplesThe samples52 wild paralarvae caught in NW Atlantic Oceanographic vessel of IEO Sampling: Multinet 200 µm, 2m diameter. Hauls of 15 min, 2 knots, at 10-20m depth 33 paralarvae (0-30 days) reared at 21ºCOptimal culture temperatureDaily increments validated in beaks of early stages up to 98 days old  Age estimations Transmitted light 400X, DIC-Nomarski Two readings. Precision calculated by Coefficient of Variation (CV-Campana, 2001)Paralarvae set Mean CVCultured 21ºC  (N=33) 6.71Cultured 14ºC  (N=37) 5.24Wild  (N=52) 4.7350 µm Reading areaRESULTS - Beak reading improvementsIdentification of the first increment  Hatchlings showed 1-3 increments:  mean values of 1.6 increments at 14ºC and 1.7 increments at 21ºCFirst incrementDelimitation of reading area: Located in the Rostrum, joined to the Hood in early stages Shoulder out of the reading areaRostrum   HoodShoulderReading areay = 1,0932x - 1,7R² = 0,986y = 0,0522x2 + 1,3248x - 1,6R² = 0,8757-5051015202530350 5 10 15 20 25 30 35Age (days)Number of incrementsRESULTS - Beak increments in captivityCold  (14ºC)  <1 increment/day  Optimal  (21ºC) = 1crement/day Growth in captivity012340 10 15 20 25 30Dry Weight of the paralarva (mg)Age (days)21ºC Artemia 14ºC Artemia21ºC14ºC20 days old  20 days old Mean environmental data for all the capture locations:DEPTH (m) 18.5TEMPERATURE (ºC) 14.3SALINITY (‰) 35.6Captures during spawning season:  May - September 2015 Ages of wild paralarvaeEstimated according results obtained at 14ºC03691215180 1 2 3 4 5 6 7 8 9 10 11Number of wild paralarvaeEstimated ageWild paralarva(4 increments)y = 0,0522x2 + 1,3248x - 1,6R² = 0,8757-505101520250 5 10 15 20 25Age (days)Number of increments Beaks of paralarvae reared at cold waters (14ºC) showed age underestimation with <1 increment/day in beaks, but at optimal temperature displayed daily increments.  Several hypothesis are feasible: • Overlapping of increments when growth slow down, as observed in experiments at 14ºC,• Periodicity is daily in culture at 21ºC but it may change with water temperatureSUMMARY & CONCLUSIONSNext steps - laboratory experiments to cover the thermal range of wild early stagesThanks for your attentionFunded by the MINECO (Spanish Government) under Project OCTOWELF (AGL2013-49101-C2-1). We thank the collaboration of Parque Nacional das Illas Atlanticas (Galicia, Spain)

Ageing early life stages of Octopus vulgaris. Effect of temperature

Perales-Raya, Catalina

Nande, Manuel

Bartolomé-Baraza, Aurora

Otero-Pinzas, Juan José

Almansa, Eduardo

Digital.CSIC

http://www.repositorio.ieo.es/e-ieo/bitstream/10508/10584/5/Perales-Raya%20et%20al_2016%20ICES2016_oral.pdf

Ageing early life stages of Octopus vulgaris. Effect of temperature

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