Determination of Mo(VI)Zr(IV) interaction parameters in highly acidic solutions by UV absorbance spectrophotometry

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Investigation of Mo(VI) monomerdimer equilibrium in highly acidic solutions by UV absorbance spectroscopy using refined numerical processing

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Proteome modification induced by differential inhibition of MsrA and MsrB in HEK293 cells

11th International Symposium on the Neurobiology and Neuroendocrinology of Aging, Bregenz, AUSTRIA, JUL 29-AUG 03, 2012International audienceno abstrac

Determination of lanthanum(III) and europium(III) – lactate interaction constants by capillary electrophoresis for environmental applications

In the present work, we investigated the ability of electrophoresis to determine the stability constant of the complex formed between lactate ions (a simple model of natural organic matter), and lanthanum (III) and europium (III), two model metal cations of actinides. The effect of lactate on lanthanide’s effective mobility is studied, and conditional metal ion-ligand interaction constants are determined on the basis of the variation in the effective mobilities (log βn = 2.23 for lanthanum (III) and 2.39 for europium (III), at 25°C and I=0.015 M). The originality of this contribution lies in the fact that we have chosen to detect the metal ion instead of the ligand by an indirect UV-visible detection and using a chromophoric compound (creatinine)

Structure, evolution and expression of the FOXL2 transcription unit. Cytogenet. Genome Res

Abstract. FOXL2 is a putative transcription factor involved in ovarian development and function. Its mutations in humans are responsible for the blepharophimosis syndrome, characterized by eyelid malformations and premature ovarian failure (POF). Here we have performed a comparative sequence analysis of FOXL2 sequences of ten vertebrate species. We demonstrate that the entire open reading frame (ORF) is under purifying selection leading to strong protein conservation. We also review recent data on FOXL2 transcript and protein expression. FOXL2 has been shown 1) to be the earliest known sex dimorphic marker of ovarian determination/differentiation in vertebrates, 2) to have, at least in mammals, an ovarian expression persisting until adulthood. The conservation of its sequence and pattern of expression suggests that FOXL2 might be a key factor in the early development of the vertebrate female gonad and involved later in adult ovarian function. Finally, we provide arguments for the existence of an alternative transcript in rodents, that may arise from a differential polyadenylation. Although it has only been demonstrated in rodents, its presence/absence in other species deserves further investigation

Towards the control of intercellular protein turnover: Mitochondrial Lon protease inhibitors versus proteasome inhibitors

Cellular protein homeostasis results from the combination of protein biogenesis processes and protein quality control mechanisms, which contribute to the functional state of cells under normal and stress conditions. Proteolysis constitutes the final step by which short-lived, misfolded and damaged intracellular proteins are eliminated. Protein turnover and oxidatively modified protein degradation are mainly achieved by the proteasome in the cytosol and nucleus of eukaryotic cells while several ATP-dependent proteases including the matrix protease Lon take part in the mitochondrial protein degradation. Moreover, Lon protease seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix. Specific inhibitors are commonly used to assess cellular functions of proteolytic systems as well as to identify their protein substrates. Here, we present and discuss known proteasome and Lon protease inhibitors. To date, very few inhibitors of Lon have been described and no specific inhibitors of this protease are available. The current knowledge on both catalytic mechanisms and inhibitors of these two proteases is first described and attempts to define specific non-peptidic inhibitors of the human Lon protease are presented

La structure de la communauté fongique associée à un individu plante est-elle déterminée par ses voisins et à quelle échelle spatio-temporelle ?

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