La Leucémie aiguë lymphoblastique (prise en charge en odontologie pédiatrique)

CLERMONT FD-BCIU-Santé (631132104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Scoring and psychometric validation of the ‘Determinants of Intentions to Vaccinate’ (DIVA©) questionnaire

International audienceBackground: Primary care physicians (PCPs) play a key role regarding vaccination in France. The aims of the present study were to define the scoring rules and to assess the measurement properties of the ‘Determinants of Intentions to Vaccinate’ (DIVA©) questionnaire that aims to assess PCPs’ attitudes and beliefs toward vaccination.Methods: The DIVA questionnaire was derived from a literature review and PCPs focus groups. Scoring and early validation of the DIVA questionnaire were determined during a cross-sectional study conducted in France. During the study, PCPs had to complete the DIVA questionnaire for any of the six vaccine-preventable diseases (VPDs) to which they were randomly assigned (measles, pertussis, pneumococcus infection, seasonal influenza, human papillomavirus -HPV- infection and tetanus). Descriptive analyses of items and the analysis of the grouping of items into domains were conducted. Internal consistency reliability and construct validity was assessed according to each VPD.Results: The DIVA questionnaire was completed by 1,069 PCPs and was well accepted. The ‘Commitment of the PCP to the vaccination approach’ score showed very good internal consistency reliability (Cronbach’s alpha >0.70 overall and for each VPD). The construct validity of the DIVA questionnaire was confirmed.Conclusions: The DIVA questionnaire is a valid and reliable measure of PCPs’ attitudes and beliefs toward vaccination

Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines

Abstract Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread quickly around the globe. Currently, no effective vaccine is available to prevent COVID-19 and an intense global development activity is in progress. In this context, the different technology platforms face several challenges resulting from the involvement of a new virus still not fully characterised. Finding of the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles is among them. Here, we describe the establishment of a workflow based on shotgun tandem mass spectrometry data to guide the optimisation of the conditions for viral amplification. In parallel, we analysed the dynamic of the host cell proteome following SARS-CoV-2 infection providing a global overview of biological processes modulated by the virus and that could be further explored to identify drug targets to address the pandemic

Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines

International audienceSevere acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic

La situation délibérative dans le débat public

Le débat public est à la fois nécessaire pour la démocratie et souvent inefficace dans ses mises en œuvre. Ainsi, les débats politiques télévisés sont, la plupart du temps, détournés en opérations de communication, et les NTIC nous proposent des forums qui se contentent de juxtaposer des opinions. L'efficacité du débat public résiderait donc, plutôt, dans la légitimité conférée au citoyen et dans l'impact de sa parole sur les processus décisionnels. Se pose ainsi le besoin d'une réflexion sur la situation délibérative dans le débat public. Motivé par les questions de la vulgarisation scientifique, le CEDP s'est positionné sur une problématique de l'usage qui renverse le schéma traditionnel de la communication. D'une démarche d'interrogation sur le public, il est passé à un positionnement théorique qui interroge le questionnement de ce même public. De nouvelles méthodes de débat public sont recherchées. Celui-ci apparaît alors comme la technique générique de production d'une « efficacité sociale » des usages de la science. À partir de trois acteurs qui, dans l'interrelation nouvelle proposée, sont légitimés (le public, le politique, l'expert), le laboratoire s'est attaché à mettre au point un protocole – les Rencontres Délibératives® – visant à rendre le grand public plus apte au débat sur la science. Le CEDP a décliné le modèle « Rencontres Délibératives » dans des champs diversifiés. Un répertoire de méthodologies employées, en fonction du champ social considéré, a été constitué. Nous avons alors abordé le problème de la propagation des savoirs et de leur appropriation par le public. Se pose aujourd'hui la question du débat public comme « apprentissage social » permettant aux publics le passage de la participation à la délibération. Ce questionnement appelle, de nos jours, une approche pluridisciplinaire. Les contributions nombreuses de ce colloque participent, en théorie et en pratique, à la constitution de cet objet scientifique