509 research outputs found
Multidimensional generalized Gini indices.
The axioms used to characterize the generalized Gini social evaluation orderings for one-dimensional distributions are extended to the multidimensional attributes case. A social evaluation ordering is shown to have a two-stage aggregation representation if these axioms and a separability assumption are satisfied. In the first stage, the distributions of each attribute are aggregated using generalized Gini social evaluation functions. The functional form of the second-stage aggregator depends on the number of attributes and on which version of a comonotonic additivity axiom is used. The implications of these results for the corresponding multidimensional indices of relative and absolute inequality are also considered.Generalized Gini; multidimensional inequality
Thermal Plasma of Electric Arc Discharge Between Composite Cu-Cr Electrsodes: Optical Emission and Electrode Surface Interaction
This work deals with investigations of thermal plasma of electric arc discharge between sintered composite Cu-Cr electrodes, which can be used in electrical contacts of vacuum circuit breakers. Breaking arcs between composite Cu-Cr as well as single-component copper electrodes were used to study the electrical properties, plasma optical emission and electrodes surface modification behavior. In particular, the temporal evolution of plasma emission spectra of electric breaking arcs in air atmosphere was investigated by Optical Emission Spectroscopy (OES). Scanning Electron Microscopy (SEM) with Energy-dispersive X-ray Spectroscopy (EDXS) were applied to analyze the cross-section of working layer of electrodes surface modified by the heat flux from the discharge
Reasons and Means to Model Preferences as Incomplete
Literature involving preferences of artificial agents or human beings often
assume their preferences can be represented using a complete transitive binary
relation. Much has been written however on different models of preferences. We
review some of the reasons that have been put forward to justify more complex
modeling, and review some of the techniques that have been proposed to obtain
models of such preferences
Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding
Pseudomonas aeruginosa employs lectins to bind to its host cells, and is known to be the major cause of lung infections. Lectin B (LecB) from Pseudomonas aeruginosa binds specifically to galactose and fucose and is important for pathogenicity, adhesion and biofilm formation. In this work, the neutron crystal structure (1.9 angstrom) of the deuterated LecB/Ca/fucose complex is reported. The structure, in combination with perdeuteration of the ligand and the receptor allowed the observation of hydrogen atoms, protonation states and hydrogen bonds involved in the interaction between pathogenic bacteria and host cells. Thus the study provides structural insights into the mechanism of high affinity binding of LecB to its targets.
The opportunistic pathogen Pseudomonas aeruginosa, a major cause of nosocomial infections, uses carbohydrate-binding proteins (lectins) as part of its binding to host cells. The fucose-binding lectin, LecB, displays a unique carbohydrate-binding site that incorporates two closely located calcium ions bridging between the ligand and protein, providing specificity and unusually high affinity. Here, we investigate the mechanisms involved in binding based on neutron crystallography studies of a fully deuterated LecB/fucose/calcium complex. The neutron structure, which includes the positions of all the hydrogen atoms, reveals that the high affinity of binding may be related to the occurrence of a low-barrier hydrogen bond induced by the proximity of the two calcium ions, the presence of coordination rings between the sugar, calcium and LecB, and the dynamic behaviour of bridging water molecules at room temperature. These key structural details may assist in the design of anti-adhesive compounds to combat multi-resistance bacterial infections
Plasma-surface interaction of electric arc discharge between composite Cu-Cr electrodes
In this work the plasma of electric arc discharges between Cu-Cr composite electrodes, which material were pressured and sintered at different temperatures 750 or 1050°C, are investigated by Optical Emission Spectroscopy (OES). The Scanning Electron Microscopy (SEM) with Energy-Dispersive X-ray Spectroscopy (EDXS) are used to study the working surface of electrodes, modified by thermal effect of breaking electrical arc in the air atmosphere. The erosion intensity of electrodes’ material due to plasma-surface interaction is estimated in indirect way by determination of metal vapours content in the discharge gap in an assumption of local thermodynamic equilibrium (LTE). The plasma composition is calculated in this case on the base of experimentally determined plasma parameters: temperature and electron density.Методами оптичної емісійної спектроскопії досліджується плазма електродугових розрядів між композитними Cu-Cr-електродами, матеріал яких y процесі виготовлення піддавався пресуванню та спіканню при різних температурах: 750 або 1050°C. Для дослідження робочої поверхні електродів, які зазнали впливу термічної дії розривної електричної дуги в атмосфері повітря, використовується скануюча електронна мікроскопія (СЕМ), доповнена енергодисперсійною рентгенівською спектроскопією (ЕДРС). Інтенсивність ерозії матеріалу електродів внаслідок взаємодії плазма-поверхня оцінювалась у непрямий спосіб шляхом визначення вмісту парів металів у розрядному проміжку в припущенні локальної термодинамічної рівноваги (ЛТР). Склад плазми в даному випадку розрахований з експериментально отриманих параметрів плазми: температури та електронної концентрації.Методами оптической эмиссионной спектроскопии (ОЭС) исследуется плазма электродуговых разрядов между композитными Cu-Cr- электродами, материал которых в процессе изготовления подвергался прессованию и спеканию при разных температурах: 750 или 1050°C. Для изучения рабочей поверхности электродов, подвергающейся термическому воздействию разрывной электрической дуги в атмосфере воздуха, использована сканирующая электронная микроскопия (СЭМ), дополненная энергодисперсионной рентгеновской спектроскопией (ЭДРС). Интенсивность эрозии материала электродов вследствие взаимодействия плазма-поверхность оценивалась косвенным методом путем определения содержания паров металлов в разрядном промежутке в предположении локального термодинамического равновесия (ЛТР). Состав плазмы в этом случае рассчитан из экспериментально полученных параметров: температуры и электронной концентрации
Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions.
Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the room temperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-π stacking interactions between fucose and the aromatic amino acids in the binding site
Candidate biomarkers of antibiotic resistance for the monitoring of wastewater and the downstream environment
Urban wastewater treatment plants (UWTPs) are essential for reducing the pollutants load and protecting water bodies. However, wastewater catchment areas and UWTPs emit continuously antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), with recognized impacts on the downstream environments. Recently, the European Commission recommended to monitor antibiotic resistance in UWTPs serving more than 100 000 population equivalents. Antibiotic resistance monitoring in environmental samples can be challenging. The expected complexity of these systems can jeopardize the interpretation capacity regarding, for instance, wastewater treatment efficiency, impacts of environmental contamination, or risks due to human exposure. Simplified monitoring frameworks will be essential for the successful implementation of analytical procedures, data analysis, and data sharing. This study aimed to test a set of biomarkers representative of ARG contamination, selected based on their frequent human association and, simultaneously, rare presence in pristine environments. In addition to the 16S rRNA gene, ten potential biomarkers (intI1, sul1, ermB, ermF, aph(3′’)-Ib, qacEΔ1, uidA, mefC, tetX, and crAssphage) were monitored in DNA extracts (n = 116) from raw wastewater, activated sludge, treated wastewater, and surface water (upstream and downstream of UWTPs) samples collected in the Czech Republic, Denmark, Israel, the Netherlands, and Portugal. Each biomarker was sensitive enough to measure decreases (on average by up to 2.5 log-units gene copy/mL) from raw wastewater to surface water, with variations in the same order of magnitude as for the 16S rRNA gene. The use of the 10 biomarkers allowed the typing of water samples whose origin or quality could be predicted in a blind test. The results show that, based on appropriate biomarkers, qPCR can be used for a cost-effective and technically accessible approach to monitoring wastewater and the downstream environment.info:eu-repo/semantics/publishedVersio
Monitoring antibiotic resistance in aquatic environments
info:eu-repo/semantics/publishedVersio
Welding dynamics in an atomistic model of an amorphous polymer blend with polymer-polymer interface
We consider an atomistic model of thermal welding at the polymer-polymer interface of a polyetherimide/polycarbonate blend, motivated by applications to 3D manufacturing in space. We follow diffusion of semiflexible chains at the interface and analyze strengthening of the samples as a function of the welding time tw by simulating the strain-stress and shear viscosity curves. The time scales for initial wetting, and for fast and slow diffusion, are revealed. It is shown that each component of the polymer blend has its own characteristic time of slow diffusion at the interface. Analysis of strainstress demonstrates saturation of the Young’s modulus at tw = 240 ns, while the tensile strength continues to increase. The shear viscosity is found to have a very weak dependence on the welding time for tw > 60 ns. It is shown that both strain-stress and shear viscosity curves agree with experimental data
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