Effect of ovarian superstimulation on COC collection and maturation in alpacas

The objective of the present study was to compare the ovarian follicular response, cumulus-oocyte complex (COC) collection rate, and maturational status of COC collected from alpacas subsequent to treatment with two different superstimulatory protocols. Alpacas (n = 7 per group) were treated with: (1) 200 mg of FSH im divided bid for 3 d, plus a single i.v. dose of 1000 IU hCG 24 h after the last FSH treatment, or (2) 1200 IU of eCG as a single i.m. dose, plus a single i.v. dose of 1000 IU of hCG on day 3 after eCG treatment (day 0 = start of superstimulatory treatment). At 20-24 h post-hCG treatment, the ovaries were surgically exposed and COC were collected by needle aspiration of all follicles ≥6 mm. The FSH and eCG treatment groups did not differ with respect to the number of follicles ≥6 mm at the time of COC collection (20.0 ± 7.5 versus 27.0 ± 3.3; P = 0.5), the number of COC collected (26.2 ± 8.4 versus 23.3 ± 3.7; P = 0.7), or the collection rate per follicle aspirated (89% versus 87%; P = 0.7). No differences were detected between FSH- and eCG-treated alpacas in the number of expanded COC collected per alpaca (11.5 ± 2.9 versus 8.8 ± 2.8; P = 0.54), the number of expanded COC in metaphase II (8.5 ± 1.9 versus 6.0 ± 2.1; P = 0.1), or the number of compact COC with ≥3 layers of cumulus cells (12.5 ± 4.3 versus 14.3 ± 2.6; P = 0.72). A greater proportion (P < 0.05) of compact COC collected after FSH treatment matured in vitro to the metaphase II stage than after eCG treatment. Eight expanded alpaca COC were fertilized in vitro with llama sperm, three of which were fixed and stained 18 h after exposure to sperm and five were cultured in vitro. Two of the three stained oocytes were in the pronuclear stage, and all five of the cultured oocytes developed to the two-cell and morula stages at 2 and 7 days, respectively, after in vitro fertilization. In summary, FSH and eCG treatments were equally effective for ovarian superstimulation and oocyte collection. Cumulus-oocyte complexes were collected from more than 80% of follicles aspirated during laparotomy. Nearly one third of the COC collected after superstimulation were in metaphase II, and more than 70% of the remaining COC progressed to metaphase II after in vitro maturation for 26 h, bringing the mean number of oocytes available for in vitro fertilization to 16 per alpaca. Preliminary results support the hypothesis that alpaca oocytes obtained after superstimulation in the absence of progesterone are developmentally competent since morulae developed from all five COC fertilized and cultured in vitro

A Short Review on Jet Identification

Jets can be used to probe the physical properties of the high energy density matter created in collisions at the Relativistic Heavy Ion Collider (RHIC). Measurements of strong suppression of inclusive hadron distributions and di-hadron correlations at high $p_{T}$ have already provided evidence for partonic energy loss. However, these measurements suffer from well-known geometric biases due to the competition of energy loss and fragmentation. These biases can be avoided if the jets are reconstructed independently of their fragmentation details - quenched or unquenched. In this paper, we discuss modern jet reconstruction algorithms (cone and sequential recombination) and their corresponding background subtraction techniques required by the high multiplicities of heavy ion collisions. We review recent results from the STAR experiment at RHIC on direct jet reconstruction in central Au+Au collisions at $\sqrt {s_{NN}}= 200$ GeV.Comment: Proceedings for the invited talk of Hot Quarks 2008, Estes Park, CO 18-23 August 200

Flavor Singlet Axial Vector Coupling of the Proton with Dynamical Wilson Fermions

We present the results of a full QCD lattice calculation of the flavor singlet axial vector coupling $G_A^1$ of the proton. The simulation has been carried out on a $16^3\times 32$ lattice at $\beta=5.6$ with $n_f=2$ dynamical Wilson fermions. It turns out that the statistical quality of the connected contribution to $G_A^1$ is excellent, whereas the disconnected part is accessible but suffers from large statistical fluctuations. Using a 1st order tadpole improved renormalization constant $Z_A$, we estimate $G_A^1 = 0.20(12)$.Comment: 13 pages, 5 eps figures, minor changes to text and citation

Nuclear shadowing at low Q^2

We re-examine the role of vector meson dominance in nuclear shadowing at low Q^2. We find that models which incorporate both vector meson and partonic mechanisms are consistent with both the magnitude and the Q^2 slope of the shadowing data.Comment: 7 pages, 2 figures; to appear in Phys. Rev.

Chaos and flights in the atom-photon interaction in cavity QED

We study dynamics of the atom-photon interaction in cavity quantum electrodynamics (QED), considering a cold two-level atom in a single-mode high-finesse standing-wave cavity as a nonlinear Hamiltonian system with three coupled degrees of freedom: translational, internal atomic, and the field. The system proves to have different types of motion including L\'{e}vy flights and chaotic walkings of an atom in a cavity. It is shown that the translational motion, related to the atom recoils, is governed by an equation of a parametric nonlinear pendulum with a frequency modulated by the Rabi oscillations. This type of dynamics is chaotic with some width of the stochastic layer that is estimated analytically. The width is fairly small for realistic values of the control parameters, the normalized detuning $\delta$ and atomic recoil frequency $\alpha$. It is demonstrated how the atom-photon dynamics with a given value of $\alpha$ depends on the values of $\delta$ and initial conditions. Two types of L\'{e}vy flights, one corresponding to the ballistic motion of the atom and another one corresponding to small oscillations in a potential well, are found. These flights influence statistical properties of the atom-photon interaction such as distribution of Poincar\'{e} recurrences and moments of the atom position $x$. The simulation shows different regimes of motion, from slightly abnormal diffusion with $\sim\tau^{1.13}$ at $\delta =1.2$ to a superdiffusion with $\sim \tau^{2.2}$ at $\delta=1.92$ that corresponds to a superballistic motion of the atom with an acceleration. The obtained results can be used to find new ways to manipulate atoms, to cool and trap them by adjusting the detuning $\delta$.Comment: 16 pages, 7 figures. To be published in Phys. Rev.

Ovarian response and embryo production in llamas treated with equine chorionic gonadotropin alone or with a progestin-releasing vaginal sponge at the time of follicular wave emergence

The objective of the study was to compare the ovulatory response and embryo production in llamas (Lama glama) treated with a single dose of equine chorionic gonadotropin (eCG) alone or combined with intravaginal medroxyprogesterone acetate (MPA) at the time of follicular wave emergence. Llamas with a growing follicle ≥7 mm in diameter were assigned to one of the following groups: (1) Control (n = 28): Nonstimulated llamas were mated and embryos were collected 7 d after mating. (2) eCG (n = 32): Llamas were given 5 mg luteinizing hormone (LH) (Day 0) to induce ovulation, 1000 IU eCG on Day 2, a luteolytic dose of prostaglandin F2α on Day 6, mating on Day 7, and embryo collection on Day 14. (3) eCG+MPA (n = 34): Llamas were treated as those in the eCG group, but a sponge containing 60 mg MPA was placed intravaginally from Days 2 to 6. Llamas that did not respond to synchronization or superstimulation were excluded, leaving data from n = 26, 26, and 27 in the control, eCG, and eCG+MPA groups, respectively, for statistical analysis. The mean (±SD) number of follicles &gt; 7 mm at the time of mating was greatest in the eCG group, intermediate in the eCG+MPA group, and lowest in the control group (16.6 ± 5.3, 12.9 ± 3.7, and 1.0 ± 0.0, respectively, P &lt; 0.001). The number of corpora lutea was similar between eCG and eCG+MPA groups (10.1 ± 2.9 and 8.6 ± 3.7, respectively); both were higher (P &lt; 0.001) than in controls (0.9 ± 0.3). The number of embryos did not differ significantly between the eCG and eCG+MPA groups (4.8 ± 2.8 and 3.5 ± 3.0, respectively), but both were higher (P &lt; 0.001) than in the controls (0.7 ± 0.4). In conclusion, eCG, with or without MPA effectively induced a superovulatory response and multiple embryo production in llamas. © 2009 Elsevier Inc. All rights reserved

Jet Reconstruction in Heavy Ion Collisions

We examine the problem of jet reconstruction at heavy-ion colliders using jet-area-based background subtraction tools as provided by FastJet. We use Monte Carlo simulations with and without quenching to study the performance of several jet algorithms, including the option of filtering, under conditions corresponding to RHIC and LHC collisions. We find that most standard algorithms perform well, though the anti-kt and filtered Cambridge/Aachen algorithms have clear advantages in terms of the reconstructed transverse-momentum offset and dispersion.Comment: 31 pages, 17 figure

Global regularity criterion for the 3D Navier-Stokes equations involving one entry of the velocity gradient tensor

In this paper we provide a sufficient condition, in terms of only one of the nine entries of the gradient tensor, i.e., the Jacobian matrix of the velocity vector field, for the global regularity of strong solutions to the three-dimensional Navier-Stokes equations in the whole space, as well as for the case of periodic boundary conditions

In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas

The objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with either: 1) 25 mg of FSH im, twice daily for 4 d; or 2) 1000 IU of eCG as a single i.m. dose. The start of gonadotropin treatment was considered Day 0. Both groups were given 5 mg of Armour Standard LH im on Day 6, and COC were collected by follicle aspiration on Day 7. Expanded COC collected from FSH- (n = 157) and eCG-treated llamas (n = 151) were fertilized in vitro using epididymal sperm, and presumptive zygotes were in vitro cultured in SOF medium for 8 d. The FSH and eCG treatment groups did not differ with respect to: the number of follicles 7 mm (16.0; 2.7 vs 14.0; 1.9, respectively; P = 0.5); the number of COC collected (11.5; 1.9 vs 9.7; 1.2; P = 0.4); the number of expanded COC (9.8; 1.4 vs 9.4; 1.2; P = 0.8); or the percentage of presumptive zygotes which developed into 2 to 8 cell stage embryos (65.3 vs 63.1), morulas (46.2 vs 42.5), or blastocysts (23.1 vs 20.5; P > 0.05). In conclusion, FSH and eCG treatments were equally effective for recovery of a high number of expanded COC which were used directly for in vitro fertilization. Furthermore, rate of embryo development was not significantly affected by the gonadotropin treatment used. © 2011 Elsevier Inc

Subprocess Size in Hard Exclusive Scattering

The interaction region of hard exclusive hadron scattering can have a large transverse size due to endpoint contributions, where one parton carries most of the hadron momentum. The endpoint region is enhanced and can dominate in processes involving multiple scattering and quark helicity flip. The endpoint Fock states have perturbatively short lifetimes and scatter softly in the target. We give plausible arguments that endpoint contributions can explain the apparent absence of color transparency in fixed angle exclusive scattering and the dimensional scaling of transverse rho photoproduction at high momentum transfer, which requires quark helicity flip. We also present a quantitative estimate of Sudakov effects.Comment: 16 pages, 4 figures, JHEP style; v2: quantitative estimate of Sudakov effects and more detailed discussion of endpoint behaviour of meson distribution amplitude added, few other clarifications, version to appear in Phys. Rev.