Cryopreservation of collared peccary (Pecari tajacu) semen using different freezing curves, straw sizes, and thawing rates

AbstractThe objective of this study was to verify the effect of different freezing curves, straw sizes, and thawing rates on the cryopreservation of collared peccary semen. Twelve ejaculates were obtained from captive adult males by electroejaculation, and evaluated for sperm motility, kinetic rating, viability, morphology, and functional membrane integrity. The ejaculates were diluted in a coconut water extender (ACP-116c) with egg yolk and glycerol, packaged into 0.25mL or 0.50mL plastic straws and cryopreserved in liquid nitrogen following a slow (−10°C/min) or a fast (−40°C/min) freezing curve. After one week, samples were thawed at 37°C/1min or 70°C/8s and evaluated as reported for fresh semen, and also for kinematic parameters (computerized analysis). A significant decrease in sperm motility and kinetic rating was observed after glycerol addition at 5°C and also after thawing for all the treatments (P<0.05). Regarding post-thaw semen variables, no differences were verified between freezing curves when the same straw size and thawing rate were taken as reference (P>0.05). In general, values for sperm characteristics found after thawing at 37°C were better preserved than at 70°C (P<0.05), both in the use of 0.25mL or 0.50mL straws, which were similar for semen packaging (P>0.05). The evaluation of the kinematic parameters of sperm motility confirmed these results at values varying from 20% to 30% motile sperm for the samples thawed at 37°C, and values fewer than 12% motile sperm for samples thawed at 70°C (P<0.05). In conclusion, we recommend the use of a fast freezing curve that reduces the time spent on the cryopreservation of collared peccary semen, which could be packaged both in 0.25mL or 0.50mL straws, but the thawing should be conducted at 37°C/1min

Curvatura Da Superfície E Seu Efeito Na Variabilidade Espacial Dos Atributos Do Solo, Pinheiral - Rj/br

Understanding the spatial variability of soil chemical and physical attributes is important for improving management practices and soil conservation. In turn, the spatial variability of soil properties results from variation in morphological relief characteristics. Thus, the purpose of this study was to evaluate the influence of landform curvature on the spatial variability of soil chemical and physical attributes in the Mar de Morros region (Pinheiral-Rio de Janeiro State, Brazil). Two adjacent landforms were selected with convex and concave curvature and sampled in a regularly spaced grid of 10 meters. A total of 56 soil samples (0-5 cm depth) from the two landforms were collected and analyzed for physical and chemical attributes. The data were analyzed using descriptive statistics and geostatistics. All chemical attributes showed random patterns of spatial variability in both landforms. The concave landform had higher values of pH and potassium and lower values of aluminum than the convex landform. In contrast, silt content showed spatial dependence in both the concave and convex landforms. Bulk density and clay showed spatial dependence in the convex landform. Bulk density and silt content increased from the shoulder to the footslope of both landforms. The results show that, for this study area, landform curvature has more influence on the spatial dependence of soil physical attributes than of soil chemical properties. © 2016, Federal University of Lavras. All rights reserved.22443143

Clinical correlation between N-terminal pro-b-type natriuretic peptide and angiographic coronary atherosclerosis

OBJECTIVES: This study aimed to investigate the clinical correlation between angiographic coronary atherosclerosis and N-terminal pro-B-type natriuretic peptide along with other known correlated factors. METHODS: In total, 153 patients with a diagnostic hypothesis of stable angina, unstable angina or acute myocardial infarction were classified as group A (patients with angiographically normal coronary arteries) or group B (patients with angiographic coronary atherosclerosis). The two groups were analyzed with respect to the following factors: gender, age, body mass index, abdominal circumference, smoking, diabetes mellitus, arterial hypertension, early family history of atherosclerosis, statin use, the presence of metabolic syndrome, clinical presentation and biochemical factors, including cholesterol, creatinine and fibrinogen plasma concentrations, monocyte counts and N-terminal pro-B-type natriuretic peptide. RESULTS: Univariate analyses comparing the two groups revealed that group B patients more frequently had diabetes, used statins and had systolic dysfunction, N-terminal pro-B-type natriuretic peptide levels ≥250 pg/mL, fibrinogen levels >;500 mg/dL and ≥501 monocytes/mm3 compared with group A patients (

1-[2-(4-methyl-7-coumarinyloxy)ethyl]-4-(5-{1-[2-(4-methyl-7-coumarinyloxy)ethyl]-1h-1, 2, 3-triazol-4-yl}pentyl)-1h-1, 2, 3-triazole

Nature often produces compounds with a high degree of symmetry to reduce structural information and complexity. Synthesis of identical twin drugs, through the linkage of two identical pharmacophoric entities, is a classical strategy to produce more potent and/or selective drugs. Herein, two units of the privileged core of the coumarin hymecromone were linked together using “click chemistry”. Synthesis of 1-[2-(4-Methyl-7-coumarinyloxy)ethyl]-4-(5-{1-[2-(4-methyl- 7-coumarinyloxy)ethyl]-1H-1, 2, 3-triazol-4-yl}pentyl)-1H-1, 2, 3-triazole was achieved by coupling of two identical units of an azido coumarin with a symmetrical alkine using copper(I)-catalyzed alkyne-azide cycloaddition reaction, in good yields and with complete regioselectivity. © 2016 by the authors; licensee MDPI, Basel, Switzerland.2016

Nonsingular FRW cosmology and nonlinear electrodynamics

The possibility to avoid the cosmic initial singularity as a consequence of nonlinear effects on the Maxwell eletromagnetic theory is discussed. For a flat FRW geometry we derive the general nonsingular solution supported by a magnetic field plus a cosmic fluid and a nonvanishing vacuum energy density. The nonsingular behavior of solutions with a time-dependent $\Lambda(t)$-term are also examined. As a general result, it is found that the functional dependence of $\Lambda(t)$ can uniquely be determined only if the magnetic field remains constant. All these models are examples of bouncing universes which may exhibit an inflationary dynamics driven by the nonlinear corrections of the magnetic field.Comment: 20 pages, 7 figure

Cosmological particle production, causal thermodynamics, and inflationary expansion

Combining the equivalence between cosmological particle creation and an effective viscous fluid pressure with the fact that the latter represents a dynamical degree of freedom within the second-order Israel-Stewart theory for imperfect fluids, we reconsider the possibility of accelerated expansion in fluid cosmology. We find an inherent self-limitation for the magnitude of an effective bulk pressure which is due to adiabatic (isentropic) particle production. For a production rate which depends quadratically on the Hubble rate we confirm the existence of solutions which describe a smooth transition from inflationary to noninflationary behavior and discuss their interpretation within the model of a decaying vacuum energy density. An alternative formulation of the effective imperfect fluid dynamics in terms of a minimally coupled scalar field is given. The corresponding potential is discussed and an entropy equivalent for the scalar field is found.Comment: 16 pages, revtex file, submitted to Phys. Rev.

Collisional equilibrium, particle production and the inflationary universe

Particle production processes in the expanding universe are described within a simple kinetic model. The equilibrium conditions for a Maxwell-Boltzmann gas with variable particle number are investigated. We find that radiation and nonrelativistic matter may be in equilibrium at the same temperature provided the matter particles are created at a rate that is half the expansion rate. Using the fact that the creation of particles is dynamically equivalent to a nonvanishing bulk pressure we calculate the backreaction of this process on the cosmological dynamics. It turns out that the `adiabatic' creation of massive particles with an equilibrium distribution for the latter necessarily implies power-law inflation. Exponential inflation in this context is shown to become inconsistent with the second law of thermodynamics after a time interval of the order of the Hubble time.Comment: 19 pages, latex, no figures, to appear in Phys.Rev.