Dynamic measurements of serum inhibin B and estradiol: a predictive evaluation of ovarian response to gonadotrophin stimulation in the early stage of IVF treatment

Objective: We dynamically measured serum inhibin B and estradiol in the early stage of hormonal stimulation to predict the ovarian response in in vitro fertilization (IVF) treatment. Methods: A total of 57 patients (<40 years of age) who underwent the first cycle of long protocol IVF or introcytoplasmic sperm injection (ICSI) treatment were included. Serum inhibin B, estradiol, follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels were measured four times: (1) on Day 3 of the menstrual cycle (basal); (2) on the day before the first administration of gonadotrophin (Gn) (Day 0); (3) on Day 1 of Gn therapy; and (4) on Day 5 of Gn therapy. Comparisons of these measurements with ovarian responses and pregnancy outcomes were made and analyzed statistically. Results: (1) On Day 1 and Day 5 of recombinant FSH (rFSH) stimulation, ovarian response, i.e., numbers of follicles, oocytes, fertilized oocytes, and embryos, had a positive correlation (r s=0.46~0.61, P=0.000) with raised inhibin B and estradiol concentrations, but a negative correlation (r s=−0.67~−0.38, P=0.000 or P<0.01) with total rFSH dose and total days of rFSH stimulation. (2) No significant variation (P>0.05) between the pregnant and non-pregnant groups on the basis of mean age or on all hormone concentrations at four times of the IVF cycle was observed. However, all the seven patients aged >35 years did not reach pregnancy. Conclusions: (1) Serum inhibin B and estradiol concentrations obtained shortly after Gn therapy may offer an accurate and early prediction of ovarian response; (2) Low levels of serum inhibin B and estradiol obtained shortly after Gn stimulation indicate the need for a longer period of Gn treatment and a higher daily dosage; (3) No obvious pregnancy difference among patients of age <35 years was found; however, IVF pregnancy outcome is significantly lower in women of age >35 years

Climates of the last three interglacials in subtropical eastern Australia inferred from wetland sediment geochemistry

Records of Australian climate during Marine Isotope Stages 5 and 7 (130–71 and 243–191 ka) are rare, preventing detailed assessments of long-term climate, drivers and ecological responses across the continent over glacial-interglacial timescales. This study presents a geochemistry-based palaeoclimate record from Fern Gully Lagoon on North Stradbroke Island (also known as Minjerribah) in subtropical eastern Australia, which records climates in MIS 7a–c, MIS 5 and much of the Holocene, in addition to MIS 4 (71–57 ka), and parts of MIS 6, MIS 3 and MIS 2 (191–130, 57–29 and 29–14 ka). Indicators of inorganic sedimentation from a 9.5 m sediment core – focussed on high-resolution estimates of sediment geochemistry supported by x-radiography, inorganic content and magnetic susceptibility – were combined with a chronology consisting of six radiocarbon (14C) and thirteen single-grain optically stimulated luminescence (OSL) ages. Hiatuses occurred at ~178–153 ka, ~36–21 ka and ~7–2 ka and likely result from the wetland drying. Low values of locally sourced aeolian materials indicate a wet MIS 7a–c and early MIS 6 before a relatively dry MIS 5. Inorganic flux during the Holocene was up to four times greater than during MIS 5, consistent with long-term interglacial drying observed in other regions, most notably in central Australia. This study highlights the importance of employing a combination of multiple dating approaches and calibrated geochemical proxies to derive climate reconstructions and to identify depositional complexities in organic-rich wetland records.C.W. Kemp, J. Tibby, L.J. Arnold, C. Barr, P.S. Gadd, J.C. Marshall, G.B. McGregor, G.E. Jacobse