RAPID, METHOD FOR DETERMINATION OF METFORMIN AND CANAGLIFLOZIN IN PLASMA BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETER, APPLICATION TO BIOEQUIVALENCE STUDY

Objective: The objective of the study was to develop and validate a rapid selective bioanalytical method for the simultaneous determination of metformin and canagliflozin in plasma by liquid chromatography-tandem mass spectrometer (LC-MS/MS) to facilitate bioequivalence study sample analysis. Stock solutions and spiking solutions were prepared accurately in methanol and 80% methanol in water, respectively. Methods: Chromatography monitored using Analyst 1.6.2 software. Method was found selective, no matrix effect, reproducible and consistent recovery, accuracy, precision, and stable in aqueous as well as extracted/matrix samples. Method found linear over the range 10–2000 ng/ml for metformin and 30–6000 ng/ml for canagliflozin. The method is successfully applied to analyze samples collected in a bioequivalence study after administration of metformin/canagliflozin 1000/150 mg to 24 healthy male volunteers. Results: Extraction was carried out using a simple solid phase extraction using mobile phase elution. 5 μl sample delivered as injection volume in 1.000 ml/min isocratic mobile phase flow on turbo ion electron spray technique for positive mode on API 4000 MS. Discussion: Chromatography achieved within 3 min using a mobile phase containing acetonitrile and 10 mM ammonium formate buffer in a ratio of 70:30 on chromolith C18 analytical column. Q1/Q3 are 130.1/70.1, 136.3/77.1, 462.2/267.2 (with ammonium adduct), and 466.4/267.1 for metformin, metformin D6, canagliflozin, and canagliflozin D4, respectively. Conclusion: Rapid, sensitive method for the simultaneous estimation of metformin and canagliflozin in plasma by LC-MS/MS is successfully developed, validated and applied to analyze 960 unknown samples of a bioequivalence study. Incurred sample reanalysis was revealed great reproducibility

Studies on the extraction and characterization of thermostable a-amylase from pericarp of Borassus indica

Thermostable a-amylase was extracted and characterized from the fruits (pericarp) of Borassus indica. Analysis on the influence of various physico-chemical parameters on the extracted enzyme revealed a Vmax of 0.793 and a Km of 0.022. The optimum temperature was found to be 370C at pH 4.5. The stability studies on enzyme activity envisaged that the enzyme is stable up to 800C and retained its activity over a wide range of pH (4.0 – 8.5). Significant enhancement in the enzyme activity was observed in the presence of metal ions like Manganese and Strontium and an insignificant decrement in the presence of Sodium ions.African Journal of Biotechnology Vol. 4 (3), pp. 289-291, 200

Shear Stiffness of Pallet Rack Upright Frames

PaIIet racks, often fabricated using cold-formed steel, are used for the storage of goods. Uprights of these racks are braced in the cross-aisle direction forming a frame, which behaves like a built-up column. Evaluation of the shear stiffness of this frame is needed to determine the buckling load. Currently two approaches prevail in the rack industry to determine the shear stiffness. The RMI code uses a theoretical formula and the FEM code requires testing. There is a considerable difference in the stiffness values determined by two approaches. The present paper describes experimental and numerical studies conducted at Oxford Brookes University to evaluate shear stiffness in an ongoing research project

Preparation of transversely isotropic test specimen of glass FRP composite - an innovative approach-I

FRP composites have attracted attention of researchers due to ever increasing demand for lighter and stronger materials from the industry, more so from aerospace and automotive sector. Researchers, particularly in academic institutions, are suffering due to non-availability of detailed information on fabrication techniques for preparing FRP test specimens that are equivalent to an analytical model. Accurate test specimen close to analytical model reduces the compulsion of going for unrealistic assumptions that takes the analysis away from reality. An easy to follow method to design, compute and achieve correct volume fraction is presented in this work. A technique for preparing and dismantling molds with commonly available materials is presented in detail. Using simple tools and tackles coupled with a few precautions followed as described herein, prospective researchers can fabricate FRP test specimen close to their requirement.&nbsp

Preparation of transversely isotropic test specimen of natural FRP composite - an innovative approach-II

Fiber Reinforced Plastic (FRP) composites can be broadly classified as synthetic and natural, based on the type of fibers incorporated. Abundantly available natural fibers like toddy palm, sisal, jute and banana are attracting the attention of researchers due to ever increasing demand for lighter, stronger and eco-friendly materials from the industry. However, natural fibers are limited in length, not so uniform in size and behave differently in different atmospheric conditions. Added to this, the inherent tendency of natural fibers to twist and curl in dry conditions poses many problems to researchers while preparing test specimens. Researchers in general and academicians in particular are handicapped by non-availability of relevant literature on fabrication techniques to prepare natural FRP composite test specimens close to their analytical models. Present paper addresses typical problems faced by researchers during preparation of unidirectional continuous fiber reinforced composite test specimen ensuring transversely isotropic nature. Using simple hand tools coupled with a few precautions taken as described herein, prospective researchers can condition natural fibers and prepare composite specimen to suit their requirements.&nbsp

Modified Assay Procedure for the Estimation of Serum Glucose using

ABSTRACT Determination of blood glucose levels is very important to know the physiological condition of the human beings as the hormonal imbalance may cause abnormalities in glucose metabolism. The traditional methods of glucose estimation by colorimetric and titrimetric methods were involved with huge expenditure and time. The modified colorimetric microwell reader method proposed in the present study was performed with small quantities of sample and reagents with the same linearity that was observed in the normal colorimetric analysis. The modified method not only reduces the cost of the test to almost one third of the normal colorimetric method but also provide an opportunity to screen the large number of samples in a short duration of time

PREVENTING BLACK HOLE ATTACK FROM MANETS USING SECRETE KEY

MANETs system made out of remote portable device that communicates by transferring on wireless medium. This system & portrayed by absence of infrastructure, without focal facilitator and central assets. Communication is conceivable by device in a system are helpful; however, it is not generally valid in disseminated compelled asset condition. Hacker can play out the malicious exercises by not following directing convention of network layer protocols, one such attack is black hole attack. In which black hole device control the routing messages and pull in the correspondence data towards it and after that drop the data. Earlier works identifies and prevent black hole attack by observing the nodes in a network, which is not practical arrangement in hostile environment. The proposed technique mitigates Black Hole Attack from routing path in MANETs by Secret Key and Hashing. Analysis of our results demonstrates that our proposed technique precisely removes the black hole attack and extend the performance of network

Prostate specific antigen: a new means as diagnostic and prognostic factor for breast cancer

ABSTRACT Prostate specific antigen (PSA) is a serine protease expressed at high levels in prostate epithelium and elevated PSA in serum is a well established marker, and also helps in management of prostate cancer. In recent times it has become widely accepted that PSA is also present in many non prostatic sources, creating doubts about the specificity of its tissue expression. Numerous studies have suggested that the molecular forms of PSA imply to signify a potential tool for the risk assessment of breast cancer. Studies have suggested new biological role of PSA in breast physiopathology. Additional studies are essential to enrol PSA indisputably as an additional means as diagnostic and prognostic tool for breast cancer. Here is the summary of how PSA has a potential to become a new diagnostic and prognostic tool

Therapeutic implications of recombinant human erythropoietin in anaemic related clinical manifestations

The introduction of recombinant human erythropoietin (RHUEPO) has revolutionised the treatment strategies for patients suffering with anaemia of chronic renal disease and chronic heart failure. Clinical studies and several observational evidences have demonstrated that RHUEPO is also useful in various non-uraemic conditions including haematological and oncological disorders, prematurity, HIV infection and preoperative therapies. The successful treatment of all the anaemic related malfunctions with recombinant human erythropoietin (RHUEPO) has become a standard treatment tool for dialysis patients and as an interesting therapeutic option for several forms of non-renal anaemia. As a conesquence of both, RHUEPO has achieved the highest annual sales worldwide and the potential of it increases its scope in the future prospective also

Pancreatic adenocarcinoma in type 2 progressive familial intrahepatic cholestasis

<p>Abstract</p> <p>Background</p> <p>BSEP disease results from mutations in ABCB11, which encodes the bile salt export pump (BSEP). BSEP disease is associated with an increased risk of hepatobiliary cancer.</p> <p>Case Presentation</p> <p>A 36 year old woman with BSEP disease developed pancreatic adenocarcinoma at age 36. She had been treated with a biliary diversion at age 18. A 1.7 × 1.3 cm mass was detected in the pancreas on abdominal CT scan. A 2 cm mass lesion was found at the neck and proximal body of the pancreas. Pathology demonstrated a grade 2-3 adenocarcinoma with invasion into the peripancreatic fat.</p> <p>Conclusions</p> <p>Clinicians should be aware of the possibility of pancreatic adenocarcinoma in patients with BSEP disease.</p