Shear-Velocity Structure and Dynamics Beneath the Sicily Channel and Surrounding Regions of the Central Mediterranean Inferred From Seismic Surface Waves

The evolution of the Sicily Channel Rift Zone (SCRZ) is thought to accommodate the regional tectonic stresses of the Calabrian subduction system. Much of the observations we have today are either limited to the surface or to the upper crust or deeper from regional seismic tomography, missing important details about the lithospheric structure and dynamics. It is unclear whether the rifting is passive from far-field extensional stresses or active from mantle upwelling beneath. We measure Rayleigh-and Love-wave phase velocities from ambient seismic noise and invert for 3-D shear-velocity and radial anisotropic models. Variations in crustal S-velocities coincide with topographic and tectonic features. The Tyrrhenian Sea has a ∼10 km thin crust, followed by the SCRZ (∼20 km). The thickest crust is beneath the Apennine-Maghrebian Mountains (∼55 km). Areas experiencing extension and intraplate volcanism have positive crustal radial anisotropy (VSH > VSV); areas experiencing compression and subduction-related volcanism have negative anisotropy. The crustal anisotropy across the Channel shows the extent of the extension. Beneath the Tyrrhenian Sea, we find very low sub-Moho S-velocities. In contrast, the SCRZ has a thin mantle lithosphere underlain by a low-velocity zone. The lithosphere-asthenosphere boundary rises from 60 km depth beneath Tunisia to ∼33 km beneath the SCRZ. Negative radial anisotropy in the upper mantle beneath the SCRZ is consistent with vertical mantle flow. We hypothesize a more active mantle upwelling beneath the rift than previously thought from an interplay between poloidal and toroidal fluxes related to the Calabrian slab, which in turn produces uplift at the surface and induces volcanism

Switching-On Survival and Repair Response Programs in Islet Transplants by Bone Marrow–Derived Vasculogenic Cells

OBJECTIVE—Vascular progenitors of bone marrow origin participate to neovascularization at sites of wound healing and transplantation. We hypothesized that the biological purpose of this bone marrow–derived vascular component is to contribute angiogenic and survival functions distinct from those provided by the local tissue-derived vasculature

Endothelium-Derived Netrin-4 Supports Pancreatic Epithelial Cell Adhesion and Differentiation through Integrins α2β1 and α3β1

BACKGROUND: Netrins have been extensively studied in the developing central nervous system as pathfinding guidance cues, and more recently in non-neural tissues where they mediate cell adhesion, migration and differentiation. Netrin-4, a distant relative of Netrins 1-3, has been proposed to affect cell fate determination in developing epithelia, though receptors mediating these functions have yet to be identified. METHODOLOGY/PRINCIPAL FINDINGS: Using human embryonic pancreatic cells as a model of developing epithelium, here we report that Netrin-4 is abundantly expressed in vascular endothelial cells and pancreatic ductal cells, and supports epithelial cell adhesion through integrins α2β1 and α3β1. Interestingly, we find that Netrin-4 recognition by embryonic pancreatic cells through integrins α2β1 and α3β1 promotes insulin and glucagon gene expression. In addition, full genome microarray analysis revealed that fetal pancreatic cell adhesion to Netrin-4 causes a prominent down-regulation of cyclins and up-regulation of negative regulators of the cell cycle. Consistent with these results, a number of other genes whose activities have been linked to developmental decisions and/or cellular differentiation are up-regulated. CONCLUSIONS/SIGNIFICANCE: Given the recognized function of blood vessels in epithelial tissue morphogenesis, our results provide a mechanism by which endothelial-derived Netrin-4 may function as a pro-differentiation cue for adjacent developing pancreatic cell populations expressing adhesion receptors α2β1 and α3β1 integrins

Molecular chaperones as therapeutic targets to counteract proteostasis defects

The health of cells is preserved by the levels and correct folding states of the proteome, which is generated and maintained by the proteostasis network, an integrated biological system consisting of several cytoprotective and degradative pathways. Indeed, the health conditions of the proteostasis network is a fundamental prerequisite to life as the inability to cope with the mismanagement of protein folding arising from genetic, epigenetic and micro-environment stress appears to trigger a whole spectrum of unrelated diseases. Here we describe the potential functional role of the proteostasis network in tumor biology and in conformational diseases debating on how the signalling branches of this biological system may be manipulated to develop more efficacious and selective therapeutic strategies. We discuss the dual strategy of these processes in modulating the folding activity of molecular chaperones in order to counteract the antithetic proteostasis deficiencies occurring in cancer and loss/gain of function diseases. Finally, we provide perspectives on how to improve the outcome of these disorders by taking advantage of proteostasis modelling

A pilot trial evaluating the efficacy of a 10% imidacloprid/2.5% moxidectin spot-on formulation in the treatment of natural nasal capillariosis in dogs.

Abstract The efficacy and safety of a spot-on formulation containing 10% imidacloprid and 2.5% moxidectin (Advocate®, Bayer Animal Health GmbH, Leverkusen, Germany) were evaluated in a pilot trial for the treatment of canine nasal capillariosis caused by Capillaria boehmi (syn. Eucoleus boehmi). Sixteen dogs copromicroscopically positive for C. boehmi eggs were confirmed, either by rhinoscopy or species-specific PCR-coupled sequencing assays, as being affected by nasal capillariosis. The animals were randomly allocated to two different study groups, i.e. one (Group T) treated with Advocate® and one (Group C) left untreated, in a ratio of 1:1. The animals underwent clinical examination and quantitative copromicroscopy for C. boehmi eggs on Days -6 and -2 (baseline) and Day 28 ± 2 (post-baseline). Animals in Group T received Advocate® on Day 0. On Day 28 ± 2 the efficacy of the treatment (Group T) or the persistence of the infection (Group C) was confirmed by rhinoscopy or, alternatively, by molecular procedures. Seven of the eight dogs in Group T were negative on Day 28 ± 2 (reduction of baseline faecal egg counts by 99.14%), while for one dog a second treatment on Day 28 ± 2 was necessary to clear the infection, as demonstrated on Day 56 ± 2 (reduction of baseline faecal egg counts by 100% in Group T). Seven animals in Group C received a rescue dose of Advocate® on Day 28 ± 2 and scored microscopically and molecularly negative for the parasite on Day 56 ± 2, thus increasing the reduction of post-baseline egg counts to 99.57% after a single administration. These promising results show that Advocate® spot-on is an effective formulation for the treatment of canine nasal capillariosis under field conditions

Selective shielding of RFID systems in presence of very high electromagnetic field levels

The paper addresses the issue of the protection of data-carrying unit of a RFID system during a microwave disinfestations treatment of industrial wood pallets. This process is carried out using microwave reverberation chambers, which represents an efficient alternative to traditional methods, also considering the velocity of the treatment. As required by safety standards, the history of the pallet must be at disposal of the user at any time, and this can be easily accomplished mounting a transponder on the pallet itself to store and retrieve its data by the contactless reader of the RFID system. The protection, against the microwave energy, of the transponder mounted on the pallet can be assured inserting it inside a selective shield that allows its operation at the working frequency. To enhance the efficiency of this selective shielding, the RFID working frequency must be chosen as low as possible. Three different solutions has been investigated in the paper: the use of aluminium thin foils, the use of a conductive elastomer and the use of conductive plastic compounds. The evaluation of these solutions has been carried out considering their loading effects on the transponder coil, their shielding efficiency in the microwave region and the compatibility between the temperature reached during the pallet treatment and the safety of the internal electronic chip. The plastic metallised compound has resulted the most suitable for the presented application