Features of the immune response in children with acute respiratory infections caused by DNA and RNA viruses

The issues of immunopathogenesis of acute respiratory viral infections (ARVI) remain relevant, despite a long history of study. The aim of the study was to investigate the characteristics of the content of some cytokines in the blood serum of children with ARVI caused by DNA and RNA-containing viruses. Material and methods. We examined 92 children with ARVI at the age from 1 to 15 years, hospitalized in the hospital of the Children’s Clinical Hospital No. 6 (Novosibirsk). In order to determine the etiological factor, a study was carried out using the RT-PCR method (test systems AmpliSensORVI-screen-FL, “InterLabService”, Russia) of mucus from the nose and throat for the presence of genetic material of viruses that cause ARVI. Determination of the content of IL-8, IL-17, IFN-γ in the blood serum in children of all groups was carried out by the method of solid-phase ELISA using commercial test systems of LLC “Cytokin” (Russia). Results and discussion. 4 groups were formed: group I (n = 20) children with ARVI caused by DNA-containing viruses (group B, C, E adenoviruses, bocaviruses); group II (n = 53) children with ARVI caused by RNA viruses (RS virus, metapneumovirus, parainfluenza viruses 1, 2, 3, 4, rhinovirus and coronoviruses); group III (n = 12) children with ARVI caused by mixed infection; group IV (n = 7) – the genetic material of the pathogen has not been isolated. Concentration of IL-8, IL-17, IFN-γ was significantly increased in all groups compared to the normative values. Higher values of IFN-γ and IL-8 were revealed in patients with DNA viruses compared with the group with RNA-containing viruses, although the excess of IFN-γ was not statistically significant. Differences in the content of IFN-γ, IL-8 and IL-17 were noted for various etiological viral agents, but they were not significant. Conclusions. The results obtained can serve as confirmation that the functional characteristics of the response of the immune system in children with ARVI are determined not so much by the etiological factor as by its individual state. With ARVI in children, the presence of genetic material from more than one virus, apparently, is not a significant potentiating factor in the activation of the immune response. At the same time, a negative PCR result with a detailed clinical picture of ARVI does not exclude the viral genesis of the disease

Изучение продукции некоторых цитокинов при вирусных инфекциях, протекающих с диарейным синдромом у детей

The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children. Materials and research methods. A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No. 6 in Novosibirsk in the first 2 days from the onset of the disease. Two groups were formed — group 1 — patients with diarrheal syndrome and signs of respiratory system damage. Group 2 — children with isolated lesions of the respiratory tract. In all children, the genetic material of viruses was determined by polymerase chain reaction (PCR) in the nose and throat washes. Also, in all children, the determination of serum levels of IL-8, IL-17, IFN-ã by ELISA was performed. Results and discussion. When studying the levels of cytokines (IFN-ã, IL-8, IL-17) in patients with signs of gastrointestinal tract lesions in comparison with isolated respiratory manifestations, no statistically significant differences were found. However, when analyzing the levels of the studied parameters in patients with diarrheal syndrome in different age groups, significant differences in the levels of IFN-ã and IL-8 were revealed.Целью исследования явилось изучение особенностей содержания некоторых цитокинов в сыворотке крови как показателей иммунного реагирования при вирусных инфекциях, сопровождающихся диарейным синдромом у детей. Материалы и методы исследования. Проведено обследование 92 детей в возрасте от 3 месяцев до 15 лет с острыми респираторными вирусными инфекциями, госпитализированных в стационар ДКБ №6 города Новосибирска в первые двое суток от начала заболевания. Сформированы две группы: I группа — пациенты с диарейным синдромом и признаками поражения респираторной системы. Группа II — дети с изолированным поражением респираторного тракта. У всех детей провели определение генетического материала вирусов методом полимеразной цепной реакции (ПЦР) в смывах из носо- и ротоглотки. Также у всех детей было проведено определение содержания в сыворотке крови IL-8, IL-17, IFN-ã методом ИФА. Результаты и обсуждение. При исследовании уровней содержания цитокинов (IFN-ã, IL-8, IL-17) у пациентов с признаками поражения желудочно-кишечного тракта в сравнении с изолированными респираторными проявлениями статистически значимых различий выявлено не было. Однако при анализе уровней исследуемых показателей у пациентов с диарейным синдромом в различных возрастных группах выявлены достоверные различия в уровне содержания IFN-ã и IL-8

Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies

Anti-glycan antibodies represent a vast and yet insufficiently investigated subpopulation of naturally occurring and adaptive antibodies in humans. Recently, a variety of glycan-based microarrays emerged, allowing high-throughput profiling of a large repertoire of antibodies. As there are no direct approaches for comparison and evaluation of multi-glycan assays we compared three glycan-based immunoassays, namely printed glycan array (PGA), fluorescent microsphere-based suspension array (SA) and ELISA for their efficacy and selectivity in profiling anti-glycan antibodies in a cohort of 48 patients with and without ovarian cancer. The ABO blood group glycan antigens were selected as well recognized ligands for sensitivity and specificity assessments. As another ligand we selected P1, a member of the P blood group system recently identified by PGA as a potential ovarian cancer biomarker. All three glyco-immunoassays reflected the known ABO blood groups with high performance. In contrast, anti-P1 antibody binding profiles displayed much lower concordance. Whilst anti-P1 antibody levels between benign controls and ovarian cancer patients were significantly discriminated using PGA (p = 0.004), we got only similar results using SA (p = 0.03) but not for ELISA. Our findings demonstrate that whilst assays were largely positively correlated, each presents unique characteristic features and should be validated by an independent patient cohort rather than another array technique. The variety between methods presumably reflects the differences in glycan presentation and the antigen/antibody ratio, assay conditions and detection technique. This indicates that the glycan-antibody interaction of interest has to guide the assay selection

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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Nurses' perceptions of aids and obstacles to the provision of optimal end of life care in ICU

Contains fulltext : 172380.pdf (publisher's version ) (Open Access

VERBAL MODEL OF MANAGEMENT OF A VULNERABLE PROCESS OF DEDICATING THE ACCESS OF USERS TO THE SOFTWARE OF THE ELECTRONIC DOCUMENT SYSTEM

Objectives Traditional methods for solving the problem of protecting the information resource of electronic document management systems based on protective basic functions (identification, authentication and verification of users, etc.) of available tools and information security subsystems have a significant drawback - inefficiency when an attacker masters the password of regular users. In order to eliminate it, it is necessary to develop new methods related to managing the weakly vulnerable process of delimiting user access not only to confidential information, but also to the software of the electronic document management system.Method One way to solve this problem is to use the modified “soft administration” method, which consists in automatically generating lists of allowed source files in the authorization matrix by reducing the lists and comparing file integrity functions with a reference list for an existing software package. This allows you to automate the process of developing access control rules and provide management of the weakly vulnerable process of access control to software tools of the electronic document management system.Result A verbal model is developed for automated control of a weakly vulnerable process of differentiating access to software tools of an electronic document management system, and a system of performance indicators for the functioning of a promising subsystem for protecting confidential information characterizing it as a control object is proposed.Conclusion The presented verbal model provides for the possibility of distributed control, in which the security administrator directly controls the specified process using a remote software tool. The formation (change) of the authority establishment matrix for managing a weakly vulnerable process of access control using a promising subsystem for protecting confidential information and the development of a system of indicators to comprehensively evaluate the effectiveness of its functioning is one of the most important tasks of protecting information from unauthorized access, guaranteeing the implementation of a security policy throughout the entire operation period secure electronic document management system