Primitive erythropoiesis in early chick embryogenesis. II. Correlation between hemoglobin synthesis and the mitotic history.

Primitive erythroblasts in the circulating blood of the chick embryo continue to divide while synthesizing hemoglobin (Hb). Hb measurements on successive generations of erythroblasts show that there is a progressive increase in the Hb content of both interphase and metaphase cells. Furthermore, for any given embryo the Hb content of metaphase cells is always significantly greater than that of interphase cells. The distribution of Hb values for metaphase cells suggests that there are six Hb classes corresponding to the number of cell cycles in the proliferative phase. The location of erythroblasts in the cell cycle was determined by combining Feulgen cytophotometry with thymidine radioautography on the same cells. Measurements of the Hb content for erythroblasts in different compartments of the cell cycle (G1, S, G2, and M) show a progressive increase through the cycle. Thus, the amount of Hb per cell is a function of the number of cell divisions since the initiation of Hb synthesis and, to a lesser degree, the stage of the cell cycle. Earlier generations of erythroblasts synthesize Hb at a faster rate than the terminal generation. Several models have been proposed to explain these findings

Early surgical closure of a large ventricular septal defect: Influence on long-term growth

AbstractThe pre- and postoperative growth patterns or 52 otherwise normal infants undergoing primary surgical closure of a large ventricular septal defect before 7 months of age were reviewed. Serial measurements of weight, length and head circumference were compiled for all patients preoperatively and in 46 long-term survivors and were expressed as Z scores (in standard deviations from the mean for age and gender).By the time of surgery at a mean age of 0.33 year, the mean weight, length and head circumference Z scores of all 52 infants were −2.9, −0.9 and −0.6, respectively, and were all significantly below normal (p < 0.001). At a mean age of 5.7 years, the mean weight, length and head circumference Z scores of 35 patients of normal birth weight were normal or varied only marginally from those of the reference population (−0.4, −0.1 and +0.5, respectively: p < 0.02, p > 0.05 and p = 0.008, respectively) and did not differ significantly in any variable from those of 44 normal siblings. However, among 11 infants with a low birth weight, all three variables remained abnormal at long-term follow-up when compared with the reference population (−1.7, −1.7 and −0.9, respectively; p < 0.001 for each) and 22 normal siblings (p < 0.008). The difference between pre- and postoperative Z scores was highly significant (p ≤ 0.004) for all three variables in the normal birth weight group, but only a significant difference in weight Z scores emerged in the low birth weight group (p < 0.001). Catch-up growth in most cases was complete within 6 to 12 months after operation.Early surgical repair of a large ventricular septal defect results in near normal long-term growth in the majority of patients. Residual growth disturbances are usually due to extracardiac factors. The present results support a policy of early surgical intervention in infants with a large ventricular septal defect

Evidence for Accretion in the High-resolution X-ray Spectrum of the T Tauri Star System Hen 3-600

We present high-resolution X-ray spectra of the multiple T Tauri star system Hen 3-600, obtained with the High Energy Transmission Grating Spectrograph on the Chandra X-ray Observatory. Two binary components were detected in the zeroth-order image. Hen 3-600-A, which has a large mid-infrared excess, is a 2-3 times fainter in X-rays than Hen 3-600-B, due to a large flare on B. The dispersed X-ray spectra of the two primary components overlap spatially; spectral analysis was performed on the combined system. Analysis of the individual spectra was limited to regions where the contributions of A and B can be disentangled. This analysis results in two lines of evidence indicating that the X-ray emission from Hen 3-600 is derived from accretion processes: line ratios of O VII indicate that the characteristic density of its X-ray-emitting plasma is large; a significant component of low-temperature plasma is present and is stronger in component A. These results are consistent with results obtained from X-ray gratings spectroscopy of more rapidly accreting systems. All of the signatures of Hen 3-600 that are potential diagnostics of accretion activity -- X-ray emission, UV excess, H-alpha emission, and weak infrared excess -- suggest that its components represent a transition phase between rapidly accreting, classical T Tauri stars and non-accreting, weak-lined T Tauri stars.Comment: latex, 27 pages, 12 figures, 6 tables; accepted by Ap

Disruption of the MyoD/p21 Pathway in Rhabdomyosarcoma

Purpose. Rhabdomyosarcoma (RMS) is an embryonal tumor thought to arise from skeletal muscle cells that fail to differentiate terminally. The majority of RMSs express MyoD, a protein essential to the differentiation of skeletal muscle. It was recently shown that during myogenesis, MyoD activates the expression of the cyclin-dependent kinase inhibitor (CDKi), p21, which itself plays a critical role in normal muscle development. To investigate the integrity of the MyoD/p21 pathway in RMS, we analyzed p21 and its relationship to MyoD expression in RMS

Detection of Molecular Hydrogen Orbiting a "Naked" T Tauri Star

Astronomers have established that for a few million years newborn stars possess disks of orbiting gas and dust. Such disks, which are likely sites of planet formation, appear to disappear once these stars reach ages of 5-10 times 10^6 yr; yet, >= 10^7 yr is thought necessary for giant planet formation. If disks dissipate in less time than is needed for giant planet formation, such planets may be rare and those known around nearby stars would be anomalies. Herein, we report the discovery of H_2 gas orbiting a weak-lined T Tauri star heretofore presumed nearly devoid of circumstellar material. We estimate that a significant amount of H_2 persists in the gas phase, but only a tiny fraction of this mass emits in the near-infrared. We propose that this star possesses an evolved disk that has escaped detection thus far because much of the dust has coagulated into planetesimals. This discovery suggests that the theory that disks are largely absent around such stars should be reconsidered. The widespread presence of such disks would indicate that planetesimals can form quickly and giant planet formation can proceed to completion before the gas in circumstellar disks disperses.Comment: latex 12 pages, including 1 figur

Dispersal of aphids, whiteflies and their natural enemies under photoselective nets

Integrated Pest Management of insects includes several control tactics, such as the use of photoselective nets, which may reduce the flight activity of insects. Limiting the dispersal of pests such as aphids and whiteflies is important because of their major role as vectors of plant viruses, while a minor impact on natural enemies is desired. In this study, we examined for the first time the dispersal ability of three vector species, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), Macrosiphum euphorbiae (Thomas) (Hemiptera: Aphididae) and Myzus persicae (Sulzer) (Hemiptera: Aphididae), in cages covered with photoselective nets. Contrary to the results obtained with aphids, the ability of the whitefly B. tabaci, to reach the target plant was reduced by photoselective nets. In a second set of experiments, the impact of UV-absorbing nets on the visual cues of two important predator species, Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) and Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae), was evaluated. The anthocorid was caught in higher numbers in traps placed under regular nets, whereas the mites preferably chose environments in which the UV radiation was attenuated. We have observed a wide range of effects that impedes generalization, although photoselective nets have a positive effect on pest management of whiteflies and aphids under protected environments