All Muddled Up

First Line: Ev'rything today is syncopated, it's too much for meFirst Line of Chorus: I get so all muddled up whenever I attempt to syncopateKey: C Majo

Courtship Behavior of the Corn Leafhopper Dalbulus maidis (DeLong & Wolcott) (Hemiptera: Cicadellidae)

Decreased Choline Acetyltransferase (ChAT) brain level is one of the main biochemical disorders in Alzheimer's Disease (AD). In rodents, recent data show that the CHAT gene can be regulated by a neural restrictive silencer factor (NRSF). The aim of the present work was to evaluate the gene and protein expression of CHAT and NRSF in frontal, temporal, entorhinal and parietal cortices of AD patient brains. Four brains from patients with AD and four brains from subjects without dementia were studied. Cerebral tissues were obtained and processed by the guanidine isothiocyanate method for RNA extraction. CHAT and NRSF gene and protein expression were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. CHAT gene expression levels were 39% lower in AD patients as compared to the control group (p 0.05, U test) than in the control subjects. These findings suggest for the first time that in the brain of AD patients high NRSF protein levels are related to low CHAT gene expression levels. " 2013, Sociedade Brasileira de Genética. Printed in Brazil.",,,,,,,,,"http://hdl.handle.net/20.500.12104/43074","http://www.scopus.com/inward/record.url?eid=2-s2.0-84874824211&partnerID=40&md5=4aef30d76862bd7c29a21895f569dd6

Sustainable bioremediation of antibacterials, metals and pathogenic DNA in water

The global antibacterial resistance requires urgent attention from different fields of engineering. Here, several unit operations were assessed in a novel water treatment train capable of remediating antibacterials, metals and pathogenic DNA to generate clean water. The analyses used 14C-respirometry, spectrometry, and a set of molecular analyses. Multiresistant bacteria hold antibacterial resistance genes (ARGs), which were harnessed for bioremediation of pollutant mixtures. Treatment efficiencies were 25–71% for 8-days with aerobic Cr(VI) reduction and removal of Cd and Pb; and 34.8% erythromycin (ERY) was biodegraded aerobically in 20 days. The anaerobic digestion (AD) bioremediated 65–73% mixed antibacterials ERY and sulfamethoxazol (SMX) in 60 days. However, high concentrations of mixed antibacterials induced inhibition of bacteria and methanogens and higher diversity of ARGs. ARGs were eliminated at 60 °C and 5.8 kPa for 10 min. The suggested coupling sequence of operations was metal, then antibacterial aerobic bioremediation, AD (yielding biomethane as energy source), recirculation of ARGs in situ, and thermo-pressure pathogenic DNA degradation.Universidad Autónoma del Estado de México proyecto No. 3883M/201

Sustainable bioremediation of antibacterials, metals and pathogenic DNA in water

The global antibacterial resistance requires urgent attention from different fields of engineering. Here, several unit operations were assessed in a novel water treatment train capable of remediating antibacterials, metals and pathogenic DNA to generate clean water. The analyses used C-14-respirometry, spectrometry, and a set of molecular analyses. Multiresistant bacteria hold antibacterial resistance genes (ARGs), which were harnessed for bioremediation of pollutant mixtures. Treatment efficiencies were 25 71% for 8-days with aerobic Cr(VI) reduction and removal of Cd and Pb; and 34.8% erythromycin (ERY) was biodegraded aerobically in 20 days. The anaerobic digestion (AD) bioremediated 65-73% mixed antibacterials ERY and sulfamethoxazol (SMX) in 60 days. However, high concentrations of mixed antibacterials induced inhibition of bacteria and methanogens and higher diversity of ARGs. ARGs were eliminated at 60 degrees C and 5.8 kPa for 10 min. The suggested coupling sequence of operations was metal, then antibacterial aerobic bioremediation, AD (yielding biomethane as energy source), recirculation of ARGs in situ, and thermo-pressure pathogenic DNA degradation. (C) 2018 Elsevier Ltd. All rights reserved