In search of a combined brucellosis and tuberculosis vaccine for cattle

Bovine brucellosis is caused by Brucella abortus. The bacterial pathogen causes economic losses because it induces abortion in cattle. Vaccination of calves with live B. abortus strain 19 induces a certain level of protection but induces persistent antibodies against cell envelope lipopolysaccharide that make it difficult to Distinguish Infected from Vaccinated Animals (DIVA). Live vaccine B. abortus strain RB51 was developed to eliminate such interfering antibodies and therefore, facilitate the differentiation of infected from vaccinated animals and help in the eradication of the disease. Vaccination with strain RB51 induces levels of protection similar to strain 19 but neither of the two vaccines give complete protection. We have been working to enhance protection induced by strain RB51 vaccine. Protective Brucella antigens can be over-expressed in strain RB51 by introducing a plasmid containing the leuB gene and the genes encoding such antigens. To avoid the expression of antibiotic resistance genes, we produced a leuB deficient strain RB51 and introduced a plasmid containing the leuB gene and the genes to be over-expressed. This new strain maintains the plasmid and has induced significantly high protection levels in mice. In addition, it allowed the construction of an RB51 vaccine strain able to express Mycobacterium bovis protective antigens so that the vaccine could protect against brucellosis and tuberculosis simultaneously

General Relativity in Electrical Engineering

In electrical engineering metamaterials have been developed that offer unprecedented control over electromagnetic fields. Here we show that general relativity lends the theoretical tools for designing devices made of such versatile materials. Given a desired device function, the theory describes the electromagnetic properties that turn this function into fact. We consider media that facilitate space-time transformations and include negative refraction. Our theory unifies the concepts operating behind the scenes of perfect invisibility devices, perfect lenses, the optical Aharonov-Bohm effect and electromagnetic analogs of the event horizon, and may lead to further applications

Amorphous silicon thin films: The ultimate lightweight space solar cell

Progress is reported with respect to the development of thin film amorphous (alpha-Si) terrestrial solar cells for space applications. Such devices promise to result in very lightweight, low cost, flexible arrays with superior end of life (EOL) performance. Each alpha-Si cell consists of a tandem arrangement of three very thin p-i-n junctions vapor deposited between film electrodes. The thickness of this entire stack is approximately 2.0 microns, resulting in a device of negligible weight, but one that must be mechanically supported for handling and fabrication into arrays. The stack is therefore presently deposited onto a large area (12 by 13 in), rigid, glass superstrate, 40 mil thick, and preliminary space qualification testing of modules so configured is underway. At the same time, a more advanced version is under development in which the thin film stack is transferred from the glass onto a thin (2.0 mil) polymer substrate to create large arrays that are truly flexible and significantly lighter than either the glassed alpha-Si version or present conventional crystalline technologies. In this paper the key processes for such effective transfer are described. In addition, both glassed (rigid) and unglassed (flexible) alpha-Si cells are studied when integrated with various advanced structures to form lightweight systems. EOL predictions are generated for the case of a 1000 W array in a standard, 10 year geosynchronous (GEO) orbit. Specific powers (W/kg), power densities (W/sq m) and total array costs ($/sq ft) are compared

Deep subcutaneous application of poly-L-lactic acid as a filler for facial lipoatrophy in HIV-infected patients

Introduction: Facial lipoatrophy is a crucial problem of HIV-infected patients undergoing highly active antiretroviral therapy (HAART). Poly-L-lactic acid (PLA), provided as New-Fill(R)/Sculptra(TM), is known as one possible treatment option. In 2004 PLA was approved by the FDA as Sculptra(TM) for the treatment of lipoatrophy of the face in HIV-infected patients. While the first trials demonstrated relevant efficacy, this was to some extent linked to unwanted effects. As the depth of injection was considered relevant in this context, the application modalities of the preparation were changed. The preparation was to be injected more deeply into subcutaneous tissue, after increased dilution. Material and Methods: To test this approach we performed a pilot study following the new recommendations in 14 patients. Results: While the efficacy turned out to be about the same, tolerability was markedly improved. The increase in facial dermal thickness was particularly obvious in those patients who had suffered from lipoatrophy for a comparatively small period of time. Conclusion: With the new recommendations to dilute PLA powder and to inject it into the deeper subcutaneous tissue nodule formation is a minor problem. However, good treatment results can only be achieved if lipoatrophy is not too intense; treatment intervals should be about 2 - 3 weeks. Copyright (C) 2005 S. Karger AG, Basel

Electromagnetic wormholes via handlebody constructions

Cloaking devices are prescriptions of electrostatic, optical or electromagnetic parameter fields (conductivity $\sigma(x)$, index of refraction $n(x)$, or electric permittivity $\epsilon(x)$ and magnetic permeability $\mu(x)$) which are piecewise smooth on $\mathbb R^3$ and singular on a hypersurface $\Sigma$, and such that objects in the region enclosed by $\Sigma$ are not detectable to external observation by waves. Here, we give related constructions of invisible tunnels, which allow electromagnetic waves to pass between possibly distant points, but with only the ends of the tunnels visible to electromagnetic imaging. Effectively, these change the topology of space with respect to solutions of Maxwell's equations, corresponding to attaching a handlebody to $\mathbb R^3$. The resulting devices thus function as electromagnetic wormholes.Comment: 25 pages, 6 figures (some color

Numerical Analysis of Three-dimensional Acoustic Cloaks and Carpets

We start by a review of the chronology of mathematical results on the Dirichlet-to-Neumann map which paved the way towards the physics of transformational acoustics. We then rederive the expression for the (anisotropic) density and bulk modulus appearing in the pressure wave equation written in the transformed coordinates. A spherical acoustic cloak consisting of an alternation of homogeneous isotropic concentric layers is further proposed based on the effective medium theory. This cloak is characterised by a low reflection and good efficiency over a large bandwidth for both near and far fields, which approximates the ideal cloak with a inhomogeneous and anisotropic distribution of material parameters. The latter suffers from singular material parameters on its inner surface. This singularity depends upon the sharpness of corners, if the cloak has an irregular boundary, e.g. a polyhedron cloak becomes more and more singular when the number of vertices increases if it is star shaped. We thus analyse the acoustic response of a non-singular spherical cloak designed by blowing up a small ball instead of a point, as proposed in [Kohn, Shen, Vogelius, Weinstein, Inverse Problems 24, 015016, 2008]. The multilayered approximation of this cloak requires less extreme densities (especially for the lowest bound). Finally, we investigate another type of non-singular cloaks, known as invisibility carpets [Li and Pendry, Phys. Rev. Lett. 101, 203901, 2008], which mimic the reflection by a flat ground.Comment: Latex, 21 pages, 7 Figures, last version submitted to Wave Motion. OCIS Codes: (000.3860) Mathematical methods in physics; (260.2110) Electromagnetic theory; (160.3918) Metamaterials; (160.1190) Anisotropic optical materials; (350.7420) Waves; (230.1040) Acousto-optical devices; (160.1050) Acousto-optical materials; (290.5839) Scattering,invisibility; (230.3205) Invisibility cloak

Mechanical Metamaterials with Negative Compressibility Transitions

When tensioned, ordinary materials expand along the direction of the applied force. Here, we explore network concepts to design metamaterials exhibiting negative compressibility transitions, during which a material undergoes contraction when tensioned (or expansion when pressured). Continuous contraction of a material in the same direction of an applied tension, and in response to this tension, is inherently unstable. The conceptually similar effect we demonstrate can be achieved, however, through destabilisations of (meta)stable equilibria of the constituents. These destabilisations give rise to a stress-induced solid-solid phase transition associated with a twisted hysteresis curve for the stress-strain relationship. The strain-driven counterpart of negative compressibility transitions is a force amplification phenomenon, where an increase in deformation induces a discontinuous increase in response force. We suggest that the proposed materials could be useful for the design of actuators, force amplifiers, micro-mechanical controls, and protective devices.Comment: Supplementary information available at http://www.nature.com/nmat/journal/v11/n7/abs/nmat3331.htm