223 research outputs found

    Evaluation of the contribution of NTFPs gathering: to rural people’s livelihoods around two timber concessions in Gabon

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    NTFP are often presented as bringing a major contribution to rural livelihoods in terms of food and cash, and this particularly for rural communities. Few data are available in Gabon to confirm this common assertion. An annual monitoring of 127 households was conducted in 14 villages around two timber concessions in the south-east and south-west regions of Gabon. Conventional socio‐economic survey tools such as focus group discussions, census and semi-structured interviews of households were used in gathering data during one year. Results reveal that rural people depend on various sources of livelihoods for food and income generation, but overall, the current contributions of vegetal NTFPs are insignificant compared to other activities. Odika (Irvingiagabonensis), atanga sauvage (Dacryodes buettneri), fungus (Termitomyces spp) and “nut” (Coula edulis) represent the main forest products that are commonly harvested by rural people, primarily for subsistence purposes while the surplus is sold. Although some efforts were made to promote the NTFP sector in the country, the results of this study suggest that: (1) the main components of the decree No.137/PR/MEFP of February 4, 2009, that prohibited the logging of five multiple use tree species over a period of 25 years should be reconsidered for revision; (2) the State authorities and partners should promote projects aiming at increasing the knowledge of the NTFP sector. These projects should contribute to the census of NTFP (for food, medicine and services), characterize their uses, the market chains of target products, and the development potential of NTFP. Such projects may help Gabon and other Congo Basin countries to fix norms/standards for a sustainable natural resource management and for enhancing their contribution to the national economy. This will be particularly relevant in the light of dwindling oil revenues and the need to diversify and promote other revenue sources in the country

    Effects of sports drinks on the maintenance of physical performance during 3 tennis matches: A randomized controlled study

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    Background: Tennis tournaments often involve playing several consecutive matches interspersed with short periods of recovery. Objective: The objective of this study was firstly to assess the impact of several successive tennis matches on the physical performance of competitive players and secondly to evaluate the potential of sports drinks to minimize the fatigue induced by repeated matches. Methods: This was a crossover, randomized controlled study. Eight male regionally-ranked tennis players participated in this study. Players underwent a series of physical tests to assess their strength, speed, power and endurance following the completion of three tennis matches each of two hours duration played over three consecutive half-days (1.5 day period for each condition). In the first condition the players consumed a sports drink before, during and after each match; in the second, they drank an identical volume of placebo water. The results obtained were compared with the third 'rest' condition in which the subjects did not play any tennis. Main outcomes measured were maximal isometric strength and fatigability of knee and elbow extensors, 20-m sprint speed, jumping height, specific repeated sprint ability test and hand grip strength. Results: The physical test results for the lower limbs showed no significant differences between the three conditions. Conversely, on the upper limbs the EMG data showed greater fatigue of the triceps brachii in the placebo condition compared to the rest condition, while the ingestion of sports drinks attenuated this fatigue. Conclusions: This study has demonstrated for the first time that, when tennis players are adequately hydrated and ingest balanced meals between matches, then no large drop in physical performance is observed even during consecutive competitive matches

    Multicentric validation of proteomic biomarkers in urine specific for diabetic nephropathy

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    Background: Urine proteome analysis is rapidly emerging as a tool for diagnosis and prognosis in disease states. For diagnosis of diabetic nephropathy (DN), urinary proteome analysis was successfully applied in a pilot study. The validity of the previously established proteomic biomarkers with respect to the diagnostic and prognostic potential was assessed on a separate set of patients recruited at three different European centers. In this case-control study of 148 Caucasian patients with diabetes mellitus type 2 and duration >= 5 years, cases of DN were defined as albuminuria >300 mg/d and diabetic retinopathy (n = 66). Controls were matched for gender and diabetes duration (n = 82). Methodology/Principal Findings: Proteome analysis was performed blinded using high-resolution capillary electrophoresis coupled with mass spectrometry (CE-MS). Data were evaluated employing the previously developed model for DN. Upon unblinding, the model for DN showed 93.8% sensitivity and 91.4% specificity, with an AUC of 0.948 (95% CI 0.898-0.978). Of 65 previously identified peptides, 60 were significantly different between cases and controls of this study. In <10% of cases and controls classification by proteome analysis not entirely resulted in the expected clinical outcome. Analysis of patient's subsequent clinical course revealed later progression to DN in some of the false positive classified DN control patients. Conclusions: These data provide the first independent confirmation that profiling of the urinary proteome by CE-MS can adequately identify subjects with DN, supporting the generalizability of this approach. The data further establish urinary collagen fragments as biomarkers for diabetes-induced renal damage that may serve as earlier and more specific biomarkers than the currently used urinary albumin

    Two-dimensional SDS-PAGE fractionation of biological samples for biomarker discovery

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    Two-dimensional electrophoresis is still a very valuable tool in proteomics, due to its reproducibility and its ability to analyze complete proteins. However, due to its sensitivity to dynamic range issues, its most suitable use in the frame of biomarker discovery is not on very complex fluids such as plasma, but rather on more proximal, simpler fluids such as CSF, urine, or secretome samples. Here, we describe the complete workflow for the analysis of such dilute samples by two-dimensional electrophoresis, starting from sample concentration, then the two-dimensional electrophoresis step per se, ending with the protein detection by fluorescence
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