Incorporation of nitrogen from crop residues into light fraction organic matter in soils with contrasting management histories

The proportion of N from crop residues entering the light-fraction organic matter (LFOM) pool was investigated in soils with contrasting soil organic matter and microbial characteristics arising from different management histories. A laboratory experiment was conducted in which 15N-labelled sugar beet, Brussels sprout or ryegrass shoots, which possessed a range of C/N contents, and hence different biochemical qualities, were incorporated into a sandy–loam soil collected from within a field (FC) or from the field margin (FM). Amounts of C and N incorporated into LFOM were determined after 112 days. The FC and FM soils had organic C contents of 0.9% and 2.5%, respectively. Addition of crop residues increased total LFOM N content and reduced its C/N in FC soil but had no effect on total LFOM N or its C/N in FM soil. Ryegrass incorporation into FC was the only treatment in which there was a net increase in LFOM C. Isotopic analysis indicated that more crop-residue-derived N became incorporated into the LFOM N pool in FM relative to FC soil, with per cent crop residue N incorporated ranging from 25.9% to 35.3% in FC and between 38.9 and 68.5 in FM. Incorporation of crop residues had a positive priming effect on pre-existing LFOM N in FM but not FC soil. We conclude that the characteristics of plant material, together with differences in soil organic matter and microbiology resulting from contrasting management, determined the amount of crop residue C and N incorporated into both HFOM and LFOM

Arbuscular mycorrhizal fungi in organic systems

Arbuscular mycorrhizal fungi (AMF) are potential contributors to plant nutrition and pathogen suppression in low input agricultural systems, although individual species of AMF vary widely in their functional attributes. Recent studies at HRI and elsewhere have suggested that in some agricultural systems inoculum of AMF is substantially lower under conventional management relative to that under organic management. Further studies have suggested that conventional management selects AMF communities with limited benefits to their plant hosts relative to those in organic systems. There is a need to investigate the generality of these findings, and their implications for the productivity of organic systems, particularly during the period following conversion to organic management. The current project was designed to pull together existing understanding of the role, and potential role, of AMF in organic systems, and to identify sites and develop methods for use in a subsequent research programme. The project had three objectives: 01 To deliver a literature review covering current knowledge of the role of AMF in conventional and organic agricultural systems. The review considered the ways in which management influences the structure and functioning of AMF communities, including their contributions under conventional and organic management, and recommendations for future research needs. 02 To establish the extent of differences in AMF inoculum between organic and conventional systems, covering a range of management practices. Paired organic and conventional fields at 12 sites from across England were selected to investigate the relationships between management, AMF communities and soil chemistry. Organic and conventionally managed soils showed no significant difference in soil chemical properties (Organic C, total N, total P, extractable P, K, Mg). However, organically managed soils had greater AMF spore numbers and root colonisation potential, and therefore higher AMF inoculum potential, than conventionally managed soil. The relative difference in AMF spore numbers between organic and conventionally managed fields increased with time since conversion. Differences in AMF inoculum potential between organic and conventionally managed fields, and between farm sites, could not be related to differences in soil chemistry. 03 To develop a method suitable for characterising AM fungus communities in soil libraries, based on 18S rRNA terminal restriction fragment length polymorphism (T-RFLP) T-RFLP was shown to provide a rapid semi-quantitative method for analysis of AMF community diversity. However it was clear that primers currently used to amplify AMF are selective and do not allow diversity of the whole AMF community to be determined. Additionally these primers amplify contaminant fungi which need to be removed from the T-RFLP profile prior to analysis. However, contaminant diversity was shown to be low. The project has identified sites and techniques which could be valuable in future research to study the role of AMF under organic management. The study has also highlighted a number of key areas in which further research is needed in order to harness AMF to improve sustainability and productivity of organic and other agricultural systems. In particular, there is a need to determine the extent to which AMF diversity varies between organic and conventional management, the rate and mechanisms by which AMF diversity increases following conversion to organic production, the relationships between AMF diversity and crop nutrition/ pathogen control, and the soil factors controlling the effectiveness of AMF inoculum

Anisotropic magnetoresistance contribution to measured domain wall resistances of in-plane magnetised (Ga,Mn)As

We demonstrate the presence of an important anisotropic magnetoresistance contribution to the domain wall resistance recently measured in thin-film (Ga,Mn)As with in-plane magnetic anisotropy. Analytic results for simple domain wall orientations supplemented by numerical results for more general cases show this previously omitted contribution can largely explain the observed negative resistance.Comment: 4 pages; submitted to Phys Rev

Light structures phototroph, bacterial and fungal communities at the soil surface

The upper few millimeters of soil harbour photosynthetic microbial communities that are structurally distinct from those of underlying bulk soil due to the presence of light. Previous studies in arid zones have demonstrated functional importance of these communities in reducing soil erosion, and enhancing carbon and nitrogen fixation. Despite being widely distributed, comparative understanding of the biodiversity of the soil surface and underlying soil is lacking, particularly in temperate zones. We investigated the establishment of soil surface communities on pasture soil in microcosms exposed to light or dark conditions, focusing on changes in phototroph, bacterial and fungal communities at the soil surface (0–3 mm) and bulk soil (3–12 mm) using ribosomal marker gene analyses. Microbial community structure changed with time and structurally similar phototrophic communities were found at the soil surface and in bulk soil in the light exposed microcosms suggesting that light can influence phototroph community structure even in the underlying bulk soil. 454 pyrosequencing showed a significant selection for diazotrophic cyanobacteria such as Nostoc punctiforme and Anabaena spp., in addition to the green alga Scenedesmus obliquus. The soil surface also harboured distinct heterotrophic bacterial and fungal communities in the presence of light, in particular, the selection for the phylum Firmicutes. However, these light driven changes in bacterial community structure did not extend to the underlying soil suggesting a discrete zone of influence, analogous to the rhizosphere

Study of the spatial variation of the biodegradation rate of the herbicide bentazone with soil depth using contrasting incubation methods

Vertical and horizontal spatial variability in the biodegradation of the herbicide bentazone was compared in sandy-loam soil from an agricultural field using sieved soil and intact soil cores. An initial experiment compared degradation at five depths between 0 and 80 cm using sieved soil. Degradation was shown to follow the first-order kinetics, and time to 50% degradation (DT50), declined progressively with soil depth from 56 d at 0–10 cm to 520 d at 70–80 cm. DT50 was significantly correlated with organic matter, pH and dehydrogenase activity. In a subsequent experiment, degradation rate was compared after 127 d in sieved soil and intact cores from 0 to 10 and 50 to 60 cm depth from 10 locations across a 160 × 90 m portion of the field. Method of incubation significantly affected mean dissipation rate, although there were relatively small differences in the amount of pesticide remaining in intact cores and sieved soil, accounting for between 4.6% and 10.6% of that added. Spatial variability in degradation rate was higher in soil from 0 to 10 cm depth relative to that from 50 and 60 cm depth in both sieved soil and intact core assessments. Patterns of spatial variability measured using cores and sieved soil were similar at 50–60 cm, but not at 0–10 cm depth. This could reflect loss of environmental context following processing of sieved soil. In particular, moisture content, which was controlled in sieved soil, was found to be variable in cores, and was significantly correlated with degradation rate in intact topsoil cores from 0 to 10 cm depth

Evaluation of rice–legume–rice cropping system on grain yield, nutrient uptake, nitrogen fixation, and chemical, physical, and biological properties of soil

To achieve higher yields and better soil quality under rice–legume–rice (RLR) rotation in a rainfed production system, we formulated integrated nutrient management (INM) comprised of Azospirillum (Azo), Rhizobium (Rh), and phosphate-solubilizing bacteria (PSB) with phosphate rock (PR), compost, and muriate of potash (MOP). Performance of bacterial bioinoculants was evaluated by determining grain yield, nitrogenase activity, uptake and balance of N, P, and Zn, changes in water stability and distribution of soil aggregates, soil organic C and pH, fungal/bacterial biomass C ratio, casting activities of earthworms, and bacterial community composition using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The performance comparison was made against the prevailing farmers’ nutrient management practices [N/P2O5/K2O at 40:20:20 kg ha−1 for rice and 20:30:20 kg ha−1 for legume as urea/single super-phosphate/MOP (urea/SSP/MOP)]. Cumulative grain yields of crops increased by 7–16% per RLR rotation and removal of N and P by six crops of 2 years rotation increased significantly (P < 0.05) in bacterial bioinoculants-based INM plots over that in compost alone or urea/SSP/MOP plots. Apparent loss of soil total N and P at 0–15 cm soil depth was minimum and apparent N gain at 15–30 cm depth was maximum in Azo/Rh plus PSB dual INM plots. Zinc uptake by rice crop and diethylenetriaminepentaacetate-extractable Zn content in soil increased significantly (P < 0.05) in bacterial bioinoculants-based INM plots compared to other nutrient management plots. Total organic C content in soil declined at 0–15 cm depth and increased at 15–30 cm depth in all nutrient management plots after a 2-year crop cycle; however, bacterial bioinoculants-based INM plots showed minimum loss and maximum gain of total organic C content in the corresponding soil depths. Water-stable aggregation and distribution of soil aggregates in 53–250- and 250–2,000 μm classes increased significantly (P < 0.05) in bacterial bioinoculants-based INM plots compared to other nutrient management plots. Fungal/bacterial biomass C ratio seems to be a more reliable indicator of C and N dynamics in acidic soils than total microbial biomass C. Compost alone or Azo/Rh plus PSB dual INM plots showed significantly (P < 0.05) higher numbers of earthworms’ casts compared to urea/SSP/MOP alone and bacterial bioinoculants with urea or SSP-applied plots. Hierarchical cluster analysis based on similarity matrix of DGGE profiles revealed changes in bacterial community composition in soils due to differences in nutrient management, and these changes were seen to occur according to the states of C and N dynamics in acidic soil under RLR rotation

Inclusion of seasonal variation in river system microbial communities and phototroph activity increases environmental relevance of laboratory chemical persistence tests

Regulatory tests assess crop protection product environmental fate and toxicity before approval for commercial use. Although globally applied laboratory tests can assess biodegradation, they lack environmental complexity. Microbial communities are subject to temporal and spatial variation, but there is little consideration of these microbial dynamics in the laboratory. Here, we investigated seasonal variation in the microbial composition of water and sediment from a UK river across a two-year time course and determined its effect on the outcome of water-sediment (OECD 308) and water-only (OECD 309) biodegradation tests, using the fungicide isopyrazam. These OECD tests are performed under dark conditions, so test systems incubated under non-UV light:dark cycles were also included to determine the impact on both inoculum characteristics and biodegradation. Isopyrazam degradation was faster when incubated under non-UV light at all collection times in water-sediment microcosms, suggesting that phototrophic communities can metabolise isopyrazam throughout the year. Degradation rate varied seasonally between inoculum collection times only in microcosms incubated in the light, but isopyrazam mineralisation to 14CO2 varied seasonally under both light and dark conditions, suggesting that heterotrophic communities may also play a role in degradation. Bacterial and phototroph communities varied across time, but there was no clear link between water or sediment microbial composition and variation in degradation rate. During the test period, inoculum microbial community composition changed, particularly in non-UV light incubated microcosms. Overall, we show that regulatory test outcome is not influenced by temporal variation in microbial community structure; however, biodegradation rates from higher tier studies with improved environmental realism, e.g. through addition of non-UV light, may be more variable. These data suggest that standardised OECD tests can provide a conservative estimate of pesticide persistence end points and that additional tests including non-UV light could help bridge the gap between standard tests and field studies

Mapping the dynamic interactions between vortex species in highly anisotropic superconductors

Here we use highly sensitive magnetisation measurements performed using a Hall probe sensor on single crystals of highly anisotropic high temperature superconductors $Bi_{2}Sr_{2}CaCu_{2}O_{8}$ to study the dynamic interactions between the two species of vortices that exist in such superconductors. We observe a remarkable and clearly delineated high temperature regime that mirrors the underlying vortex phase diagram. Our results map out the parameter space over which these dynamic interaction processes can be used to create vortex ratchets, pumps and other fluxonic devices.Comment: 7 pages, 3 figures, to be published in Supercond. Sci. Techno

Angular dependence of domain wall resistivity in artificial magnetic domain structures

We exploit the ability to precisely control the magnetic domain structure of perpendicularly magnetized Pt/Co/Pt trilayers to fabricate artificial domain wall arrays and study their transport properties. The scaling behaviour of this model system confirms the intrinsic domain wall origin of the magnetoresistance, and systematic studies using domains patterned at various angles to the current flow are excellently described by an angular-dependent resistivity tensor containing perpendicular and parallel domain wall resistivities. We find that the latter are fully consistent with Levy-Zhang theory, which allows us to estimate the ratio of minority to majority spin carrier resistivities, rho-down/rho-up~5.5, in good agreement with thin film band structure calculations.Comment: 14 pages, 3 figure

Equilibrium properties of the mixed state in superconducting niobium in a transverse magnetic field: Experiment and theoretical model

Equilibrium magnetic properties of the mixed state in type-II superconductors were measured with high purity bulk and film niobium samples in parallel and perpendicular magnetic fields using dc magnetometry and scanning Hall-probe microscopy. Equilibrium magnetization data for the perpendicular geometry were obtained for the first time. It was found that none of the existing theories is consistent with these new data. To address this problem, a theoretical model is developed and experimentally validated. The new model describes the mixed state in an averaged limit, i.e. %without detailing the samples' magnetic structure and therefore ignoring interactions between vortices. It is quantitatively consistent with the data obtained in a perpendicular field and provides new insights on properties of vortices. % and the entire mixed state. At low values of the Ginzburg-Landau parameter, the model converts to that of Peierls and London for the intermediate state in type-I superconductors. It is shown that description of the vortex matter in superconductors in terms of a 2D gas is more appropriate than the frequently used crystal- and glass-like scenarios.Comment: 8 pages, 9 figure