Cell distribution after intracoronary bone marrow stem cell delivery in damaged and undamaged myocardium: implications for clinical trials

International audienceABSTRACT : INTRODUCTION : Early randomized clinical trials of autologous bone marrow cardiac stem cell therapy have reported contradictory results highlighting the need for a better evaluation of protocol designs. This study was designed to quantify and compare whole body and heart cell distribution after intracoronary or peripheral intravenous injection of autologous bone marrow mononuclear cells in a porcine acute myocardial infarction model with late reperfusion. METHODS : Myocardial infarction was induced using balloon inflation in the left coronary artery in domestic pigs. At seven days post-myocardial infarction, 1 x 10(8) autologous bone marrow mononuclear cells were labeled with fluorescent marker and/or 99mTc radiotracer, and delivered using intracoronary or peripheral intravenous injection (leg vein). RESULTS : Scintigraphic analyses and Upsilon-emission radioactivity counting of harvested organs showed a significant cell fraction retained within the heart after intracoronary injection (6 +/- 1.7% of injected radioactivity at 24 hours), whereas following peripheral intravenous cell injection, no cardiac homing was observed at 24 hours and cells were mainly detected within the lungs. Importantly, no difference was observed in the percentage of retained cells within the myocardium in the presence or absence of myocardial infarction. Histological evaluation did not show arterial occlusion in both animal groups and confirmed the presence of bone marrow mononuclear cells within the injected myocardium area. CONCLUSIONS : Intravenous bone marrow mononuclear cell injection was ineffective to target myocardium. Myocardial cell distribution following intracoronary injection did not depend on myocardial infarction presence, a factor that could be useful for cardiac cell therapy in patients with chronic heart failure of non-ischemic origin or with ischemic myocardium without myocardial infarction

Experimental Study of electron acceleration by plasma beat-waves with Nd lasers

International audienceWe have observed the acceleration of electrons by a beat-wave generated in a deuterium plasma by two Nd-YAG and Nd-YLF laser wavelengths. Electrons injected at an energy of 3.3 MeV are observed to be accelerated up to 4.7 MeV after the plasma. The energy gain is compatible with a peak electric field of the order of 1.2 GV/m. The experiment has been performed with different injection energies, from 2.5 to 3.3 MeV, with different plasma dimensions, and with different laser intensitie

Guide d’interpretation des radiographies chez le chien et le chat

La présente étude est une étude qui a pour but de réaliser un guide d’interprétation en vue de faciliter la compréhension et l’interprétation des clichés radiographiques chez les carnivores domestiques (chien et chat).Elle s’est déroulée tout au long du mois de juillet 2016. Des radiographies de thorax, abdomen, et des structures osseuses (coude et articulation coxo-fémorale) ont été effectuées sur des carnivores domestiques (chien et chat).Après un rappel anatomique, les radiographies normales du thorax, de l’abdomen, sans produit de contraste, et du squelette sont présentées. Chaque radiographie est accompagnée d’une description de la technique d’obtention du cliché, de l’anatomie radiographique normale.Mots clés: Radiogrraphie, chien, chatEnglish Title: Interpretation guide for x-rays in dog and catsEnglish AbstractThe present study is a study that aims to provide an interpretation guide to facilitate the understanding and interpretation of X-ray images in domestic carnivores (dogs and cats). It took place throughout July 2016. X-rays of thorax, abdomen, and bone structures (elbow and hip joint) were performed on domestic carnivores (dogs and cats).After anatomic recall, the chest, abdomen, contrast-free, and skeletal X-rays are presented. Each x-ray is accompanied by a description of the technique for obtaining the radiograph, the normal radiographic anatomy.Keywords: Radiography, dog, ca

Degenerative lumbar disc disease: in vivo data support the rationale for the selection of appropriate animal models

International audienc