Ghrelin inhibits insulin secretion through the AMPK–UCP2 pathway in β cells

AbstractGhrelin inhibits insulin secretion partly via induction of IA-2β. However, the orexigenic effect of ghrelin is mediated by the AMP-activated protein kinase (AMPK)–uncoupling protein 2 (UCP2) pathway. Here, we demonstrate that ghrelin’s inhibitory effect on insulin secretion also occurs through the AMPK-UCP2 pathway. Ghrelin increased AMPK phosphorylation and UCP2 mRNA expression in MIN6 insulinoma cells. Overexpression or downregulation of UCP2 attenuated or enhanced insulin secretion, respectively. Furthermore, AMPK activator had a similar effect to ghrelin on UCP2 and insulin secretion in MIN6 cells. In conclusion, ghrelin’s inhibitory effect on insulin secretion is partly mediated by the AMPK-UCP2 pathway, which is independent of the IA-2β pathway

Charge disproportionation in the spin-liquid candidate κ − (ET)2Cu2(CN)3 at 6 K revealed by 63Cu NQR measurements

The spin-liquid candidate κ−(ET)2Cu2(CN)3 [ET: bis(ethylenedithio)tetrathiafulvalene] does not exhibit magnetic ordering down to a very low temperature, but shows a mysterious anomaly at 6 K. The origin of the so-called 6-K anomaly is still under debate. We carried out nuclear quadrupole resonance (NQR) measurements on the copper sites of the insulating layers, which are sensitive to the charge dynamics unlike conventional spin-1/2 nuclear magnetic resonance (NMR). The main finding of this Rapid Communication is that the observation of a sharp peak behavior in the nuclear spin-lattice relaxation rate T−11 of 63Cu NQR at 6 K while T−11 of both 13C and 1H NMR show no clear anomaly. This behavior can be understood as a second-order phase transition related to charge disproportionation in the ET layers

Toward the Cross-Institutional Data Integration From Shibboleth Federated LMS

Through this study, we aim to examine a method for data integration in shared Learning Management System (LMS) in authentication federation. We proposed a method of transmitting ePTID and learning data with user’s consent as a method for data integration across institutions. The method is compared with the other existing methods to realize the shared LMS. We discuss the suitable method for next version of GakuNinMoodle and conclude that our requirements are not fully satisfied by a single method

Disruption of GM2/GD2 synthase gene resulted in overt expression of 9-O-acetyl GD3 irrespective of Tis211

GM2/GD2 synthase gene knockout mice lack all complex gangliosides, which are abundantly expressed in the nervous systems of vertebrates. In turn, they have increased precursor structures GM3 and GD3, probably replacing the roles of the depleted complex gangliosides. In this study, we found that 9-O-acetyl GD3 is also highly expressed as one of the major glycosphingolipids accumulating in the nervous tissues of the mutant mice. The identity of the novel component was confirmed by neuraminidase treatment, thin layer chromatography-immunostaining, two-dimensional thin layer chromatography with base treatment, and mass spectrometry. All candidate factors reported to be possible inducer of 9-O- acetylation, such as bitamine D binding protein, acetyl CoA transporter, or O-acetyl ganglioside synthase were not up-regulated. Tis21 which had been reported to be a 9-O-acetylation inducer was partially down-regulated in the null mutants, suggesting that Tis21 is not involved in the induction of 9-O-acetyl-GD3 and that accumulated high amount of GD3 might be the main factor for the dramatic increase of 9-O-acetyl GD3. The ability to acetylate exogenously added GD3 in the normal mouse astrocytes was examined, showing that the wild-type brain might be able to synthesize very low levels of 9-O-acetyl GD3. Increased 9-O-acetyl GD3, in addition to GM3 and GD3, may play an important role in the compensation for deleted complex gangliosides in the mutant mice

Photosynthetic rates of four tree species in the upper canopy of a tropical rain forest at the Pasoh Forest Reserve in Peninsular Malaysia

Measurements of photosynthesis (A) and stomatal conductance (gs) were conducted using the leaves of four tropical tree species (Dipterocarpus sublamellatus, Neobalanocarpus heimii, Ptychopyxis caput-medusae, and Xanthophyllum amoneum) in the canopy of the lowland forest at the Pasoh Forest Reserve in peninsular Malaysia. A canopy walkway was used to reach the canopy of 30- to 40-m-tall trees and diurnal changes in A and gs were determined. The diurnal patterns for A differed between days and between species, though A of the four species were similar but quite low. In general, A increased with increasing photosynthetically active radiation (PAR) before noon, then declined. As well, a linear relationship was detected between gs and A, but no statistically significant correlation could be confirmed between these two factors for D. sublamellatus and P. caput-medusae in November and July. The value of gs decreased with increasing vapor pressure deficit for D. sublamellatus, but the other species did not show this response