Leiomyosarcoma of the Inferior Vena Cava: a case report of complete and sustained response with Trabectedin

Inferior vena cava leiomyosarcoma is a very rare tumor, accounting for only 0.5% of all soft tissue sarcomas. As the other leyomiosarcomas of vascular origin, they have a poor prognosis, and radical resection with surgical margins free of tumor is the only potentially curative treatment. We present a case of a 46 year-old woman with metastatic inferior vena cava leiomyosarcoma who progressed after anthracyclines and ifosfamide and achieved a complete and sustained response with trabectedin. Beyond progression, the patient started third line treatment with pazopanib. A brief review of literature is also given. This case supports the efectiveness of a recent therapeutic agent, with an impressive progression-free survival in a recurrent metastatic inferior vena cava leiomyosarcoma

A importância dos animais nas propriedades familiares rurais agroecológicas.

Analisou-se a integração animal nas propriedades rurais familiares agroecológicas a partir de projetos de pesquisa em interface com a extensão. Para avaliar os impactos das mudanças utilizou-se de avaliações participativas, pesquisas domiciliares, monitoramento do desempenho produtivo, entrevistas com informantes-chave e análise de conteúdo. Concluiu-se que o objetivo inicial de melhorar a integração animal com vistas à produção de esterco desencadeou diferentes idéias, projetos e perspectivas tanto nos agricultores quanto nos pesquisadores. As atividades realizadas promoveram maior consciência agroecológica, principalmente em relação à diversificação e o inter-relacionamento das atividades, assim como o respeito à natureza; permitiram também elevar a auto-estima das mulheres, por meio das atividades nas hortas e beneficiamento do leite e; melhorou a condição de vida das famílias através da diversidade e qualidade alimentar, conhecimento de aspectos sanitários e aumento de renda

Vigilância laboratorial da tuberculose em Portugal: relatório 2012

Em Portugal, e de acordo com o último relatório do Programa Nacional de luta contra a Tuberculose da Direção-Geral da Saúde, em 2012 foram notificados 2480 casos de tuberculose (TB), dos quais 2286 correspondem a casos novos (taxa de incidência de 21,6/100.000 habitantes). Embora Portugal continue entre os países de incidência intermédia, o único na Europa Ocidental, a incidência dos casos novos parece vir a diminuir desde o início da década de 2000. A incidência de tuberculose multirresistente (TB-MR) tem também vindo a diminuir. Em 2012 a incidência de TB-MR foi de 0,56% (14 casos) do total dos casos de TB registados. Esta é uma proporção inferior à média na UE e encontra-se praticamente circunscrita às áreas metropolitanas do Porto e, principalmente, de Lisboa. De acordo com os dados deste mesmo relatório, o número de casos de TB confirmados dos quais se conhece o perfil de suscetibilidade aos antibacilares tem vindo a diminuir, sendo que é cada vez mais frequente os doentes iniciarem tratamento empiricamente com base apenas em critérios clínicos e radiológicos. De facto, não sendo os laboratórios do INSA os únicos a nível nacional a efetuar isolamento em cultura e respetivo teste de suscetibilidade aos antibacilares (TSA), pode verificar-se, pela análise dos dados laboratoriais, que, embora o número de casos de TB notificados tenha aumentado em 2012, o número de casos de TB confirmados no INSA e com resultado de TSA tem vindo a diminuir. Por outro lado, e em relação aos casos de TB-MR verificamos que, entre 2008-2011, o número de casos notificados ao SVIG tem sido discordante em relação ao número de casos com resultado laboratorial. Em 2012 acentua-se essa diferença,com aumento do número de casos de TB-MR com confirmação laboratorial e que se mantêm sem notificação clínica. Este facto, alerta por si só, para a importância de se avaliarem periodicamente os vários componentes dos sistemas de vigilância, concretamente, no que diz respeito aos atrasos de notificação e consequentes implicações nas decisões a adoptar no controlo e prevenção da transmissão da tuberculose

miRNome profiling of clonal stem cells in Ph+ CML

Chronic myeloid leukemia (CML) is a myeloid stem cell neoplasm characterized by an expansion of myeloid progenitor cells and the presence of BCR-ABL1 oncoprotein. Since the introduction of specific BCR-ABL1 tyrosine kinase inhibitors (TKI), overall survival has improved significantly. However, under long-term therapy patients may have residual disease that originates from TKI-resistant leukemic stem cells (LSC). In this work, we analyzed the miRNome of CML LSC, normal hematopoietic stem cells (HSC) obtained from the same CML patients, and stem and progenitor cells obtained from healthy donors (HD) by next-generation sequencing. We detected a global decrease of microRNA levels in LSC and HSC from CML patients, and decreased levels of microRNAs and snoRNAs from a genomic cluster in chromosome 14, suggesting a mechanism of silencing of multiple non-coding RNAs. Surprisingly, HSC from CML patients, despite the absence of BCR-ABL1 expression, showed an altered miRNome. In silico analysis revealed an association between validated microRNAs and multiple metabolic pathways, suggesting that these molecules may be mediators of the previously reported dysregulation of LSC metabolism. This is the first report of the LSC miRNome that distinguishes between BCR-ABL1+ LSC and their BCR-ABL1- counterparts, providing valuable data for future studies.Fil: Ruiz, María Sol. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez, María Belén. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Bonecker, Simone. Instituto Nacional de Câncer; BrasilFil: Furtado, Carolina. Instituto Nacional de Câncer; BrasilFil: Koile, Daniel Isaac. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Yankilevich, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Cranco, Santiago. Instituto Alexander Fleming; ArgentinaFil: Custidiano, María del Rosario. Instituto Alexander Fleming; ArgentinaFil: Freitas, Josefina. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Moiraghi, Beatriz. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Pérez, Mariel Ana. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal General de Agudos "prof. Dr. Rodolfo Rossi".; ArgentinaFil: Pavlovsky, Carolina. Fundación Para Combatir la Leucemia; ArgentinaFil: Varela, Ana Inés. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Ventriglia, Verónica. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Sánchez Ávalos, Julio César Américo. Instituto Alexander Fleming; ArgentinaFil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Zalcberg, Ilana. Instituto Nacional de Câncer; BrasilFil: Mordoh, Jose. Fundación Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valent, Peter. Medical University of Vienna; AustriaFil: Bianchini, Michele. Fundación Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Fungal Planet description sheets : 320–370

Novel species of fungi described in the present study include the following from Malaysia: Castanediella eucalypti from Eucalyptus pellita, Codinaea acacia from Acacia mangium, Emarcea eucalyptigena from Eucalyptus brassiana, Myrtapenidiella eucalyptorum from Eucalyptus pellita, Pilidiella eucalyptigena from Eucalyptus brassiana and Strelitziana malaysiana from Acacia mangium. Furthermore, Stachybotrys sansevieriicola is described from Sansevieria ehrenbergii (Tanzania), Phacidium grevilleae from Grevillea robusta (Uganda), Graphium jumulu from Adansonia gregorii and Ophiostoma eucalyptigena from Eucalyptus marginata (Australia), Pleurophoma ossicola from bone and Plectosphaerella populi from Populus nigra (Germany), Colletotrichum neosansevieriae from Sansevieria trifasciata, Elsinoë othonnae from Othonna quinquedentata and Zeloasperisporium cliviae (Zeloasperisporiaceae fam. nov.) from Clivia sp. (South Africa), Neodevriesia pakbiae, Phaeophleospora hymenocallidis and Phaeophleospora hymenocallidicola on leaves of a fern (Thailand), Melanconium elaeidicola from Elaeis guineensis (Indonesia), Hormonema viticola from Vitis vinifera (Canary Islands), Chlorophyllum pseudoglobossum from a grassland (India), Triadelphia disseminata from an immunocompromised patient (Saudi Arabia), Colletotrichum abscissum from Citrus (Brazil), Polyschema sclerotigenum and Phialemonium limoniforme from human patients (USA), Cadophora vitícola from Vitis vinifera (Spain), Entoloma flavovelutinum and Bolbitius aurantiorugosus from soil (Vietnam), Rhizopogon granuloflavus from soil (Cape Verde Islands), Tulasnella eremophila from Euphorbia officinarum subsp. echinus (Morocco), Verrucostoma martinicensis from Danaea elliptica (French West Indies), Metschnikowia colchici from Colchicum autumnale (Bulgaria), Thelebolus microcarpus from soil (Argentina) and Ceratocystis adelpha from Theobroma cacao (Ecuador). Myrmecridium iridis (Myrmecridiales ord. nov., Myrmecridiaceae fam. nov.) is also described from Iris sp. (The Netherlands). Novel genera include (Ascomycetes): Budhanggurabania from Cynodon dactylon (Australia), Soloacrosporiella, Xenocamarosporium, Neostrelitziana and Castanediella from Acacia mangium and Sabahriopsis from Eucalyptus brassiana (Malaysia), Readerielliopsis from basidiomata of Fuscoporia wahlbergii (French Guyana), Neoplatysporoides from Aloe ferox (Tanzania), Wojnowiciella, Chrysofolia and Neoeriomycopsis from Eucalyptus (Colombia), Neophaeomoniella from Eucalyptus globulus (USA), Pseudophaeomoniella from Olea europaea (Italy), Paraphaeomoniella from Encephalartos altensteinii, Aequabiliella, Celerioriella and Minutiella from Prunus (South Africa). Tephrocybella (Basidiomycetes) represents a novel genus from wood (Italy). Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa.Alina V. Alexandrova was supported by the Russian Science Foundation (project N 14-50-00029). Ekaterina F. Malysheva, Olga V. Morozova, Alexander E. Kovalenko and Eugene S. Popov acknowledge financial support from the Russian Foundation for Basic Research (project 13-04-00838a and 15-04-04645a). Margarita Dueñas, María P. Martín and M. Teresa Telleria acknowledge financial support from the Plan Nacional I+D+I projects No. CGL2009-07231 and CGL2012-3559. Cony Decock gratefully acknowledges the financial support received from the FNRS / FRFC (convention FRFC 2.4544.10), the CNRS-French Guiana and the Nouragues staff, which enabled fieldwork in French Guiana, and the Belgian State – Belgian Federal Science Policy through the BCCMTM research programme.http://www.ingentaconnect.com/content/nhn/pimjam201

Removal of Cadmium by <em>Halobacterium </em> strain R1 MTCC 3265 from saline and non-saline econiches

2215-2219Haloarchaeon Halobacterium strain R1 MTCC 3265  is an attractive candidate for use in bioremediation due to its resistance to 4 mM cadmium (Cd) in nutrient rich medium NTYE (25% NaCl Tryptone Yeast extract) and up to 2 mM Cd in synthetic medium NGSM (20% NaCl glucose synthetic medium) containing 0.2% glucose as sole source of carbon. The cells accumulated a maximum of 18 ppm of Cd on the 5th day of growth. In line with its true haloarchaeal nature the cells of Halobacterium strain R1 lysed on suspension in distilled water or media containing low NaCl concentrations.  As such this strain cannot be used for the removal of metal from non-saline environments.  However to overcome this problem cells were immobilised in calcium alginate. The immobilized cells were viable and could efficiently remove up to 63% Cd from deionized water

Retrieval of euryhaline eubacterial and haloarchaeal bionts from nine different benthic sponges: reflection of the bacteriological health of waters of Mandapam, India

773-783Bacterial bionts from nine sponges from intertidal reaches of Mandapam (9o17’0" N and 7o7’0"E), namely: Petrosia testudinaria, Cinachyra cavernosa, Haliclona sp., Callyspongia fibrosa, Heteronema erecta, Fasciospongia cavernosa, Callyspongia reticutis var solomonensis unidentified sponges (MAMS), and unidentified sponge (NIO3) were enumerated by the acridine orange and viable count in Tryptone Yeast Extract medium (TYE) with 3-25% NaCl. Forty two pure bionts were characterized to generic level according to Bergey’s Systematic Bacteriology and sarted into groups using SYSTAT-v.12.01. Diversity, richness and evenness of genera were determined using PRIMER v.5. Direct bacterial counts for sponges, averaged at 5.2±2.20×109 cells/g. Viable counts averaged at 3.31±2.60×108 cfu/g, 3.83±2.86×108 cfu/g and 2.89±2.3×108 cfu/g on TYE, 3% TYE and NTYE, respectively. Predominant Genera were in the order of Bacillus>Corynebacterium > Chromohalobacter. Bionts of Genera Loktanella sp., Pontibacillus sp., Planococcus sp., Enterococcus sp. are retrieved for the first time from sponges of Mandapam. Retrieval of bionts of Genera Enterococcus, Corynebacterium, Enterobacter and Pseudomonas known for pathogenicity to humans, reflects pollution of Mandapam waters by sewage and Heralds Caution for safeguarding the waters to ensure sustainability of biota

Robust Transmission of Motion Capture Data using Interleaved LDPC and Inverse Kinematics

International audienceRecent advances in smart-sensor technology have improved precision in Motion Capture (MoCap) data for realistic animation.However, precision also imposes challenges on bandwidth. While research efforts have focussed on MoCap compression inrecent years, little attention has been given to lossy transmission taking advantage of the human perceptual threshold, whichallows many online applications, e.g., interactive games, on-demand broadcast, movies and tutoring using dynamic motionsequences. Given the growing applications on mobile devices and wireless networks, associated with insufficient bandwidth,unreliable connection and potential interference or shadowing, data loss is inevitable. We introduce a new Representationfor MoCap data, integrating Interleaved Low-Density Parity-Check (I-LDPC), with Keyframe-based Interpolation and InverseKinematics, to better address the problem of MoCap data loss during transmission. We believe this is the first study to addressrobust transmission of MoCap data considering loss. Experimental results assessed using mean opinion scores demonstratethat our approach achieves substantial improvement over alternative transmission methods.</p