1,811 research outputs found
General Aviation Turbine Engine (GATE) study
The feasibility of turbine engines for the smaller general aviation aircraft was investigated and a technology program for developing the necessary technology was identified. Major results included the definition of the 1988 general aviation market, the identification of turboprop and turboshaft engines that meet the requirements of the aircraft studies, a benefit analysis showing the superiority of gas turbine engines for portions of the market studied, and detailed plans for the development of the necessary technology
The Adventist Schools: Development and Challenges
The Seventh-day Adventist denomination operates schools and colleges around the globe. Beginning in 1853 with a small home school, the churchâs systems includes over 4,500 elementary and 1,100 secondary schools, as well as 95 colleges and universities. This article briefly presents the development of this system and the philosophy that undergirds the education offered by the schools. Maintaining the unique identity of the Adventist schools is a special challenge for the future. Other challenges faced by the schools are briefly discussed
A new approach to budgeting and financial management
A book review of Zero-Base Budgeting: A Practical Management Tool for Evaluating Expenses, by Peter A. Phyrr
DNA-Modified Electrodes Fabricated Using Copper-Free Click Chemistry for Enhanced Protein Detection
A method of DNA monolayer formation has been developed using copper-free click chemistry that yields enhanced surface homogeneity and enables variation in the amount of DNA assembled; extremely low-density DNA monolayers, with as little as 5% of the monolayer being DNA, have been formed. These DNA-modified electrodes (DMEs) were characterized visually, with AFM, and electrochemically, and were found to facilitate DNA-mediated reduction of a distally bound redox probe. These low-density monolayers were found to be more homogeneous than traditional thiol-modified DNA monolayers, with greater helix accessibility through an increased surface area-to-volume ratio. Protein binding efficiency of the transcriptional activator TATA-binding protein (TBP) was also investigated on these surfaces and compared to that on DNA monolayers formed with standard thiol-modified DNA. Our low-density monolayers were found to be extremely sensitive to TBP binding, with a signal decrease in excess of 75% for 150 nM protein. This protein was detectable at 4 nM, on the order of its dissociation constant, with our low-density monolayers. The improved DNA helix accessibility and sensitivity of our low-density DNA monolayers to TBP binding reflects the general utility of this method of DNA monolayer formation for DNA-based electrochemical sensor development
A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport
We have developed a thin layer, multiplexed biosensing platform that features two working-electrode arrays for detecting small molecules, nucleic acid sequences, and DNA-binding proteins. DNA duplexes are patterned onto the primary electrode array, while a secondary electrode array is used both to initiate DNA monolayer formation and for electrochemical readout via DNA-mediated charge transport (DNA CT) chemistry. Electrochemical reduction of Cu(phendione)_2^(2+) (phendione is 1,10-phenanthroline-5,6-dione) at the secondary electrodes induces covalent attachment via click chemistry of ethynyl-labeled DNA probe duplexes onto the primary electrodes that have been treated with azide-terminated alkylthiols. Electrochemical impedance spectroscopy and cyclic voltammetry confirm that catalyst activation at the secondary electrode is essential to maintain the integrity of the DNA monolayer. Electrochemical readout of DNA CT processes that occur at the primary electrode is accomplished also at the secondary electrode. The two-electrode system enables the platform to function as a collectorâgenerator using either ferrocyanide or ferricyanide as mediators with methylene blue and DNA charge transport. Electrochemical measurements at the secondary electrode eliminate the need for large background corrections. The resulting sensitivity of this platform enables the reliable and simultaneous detection of femtomoles of the transcription factors TATA-binding protein and CopG on a single multiplexed device
Magnetospheric particle acceleration and X-ray emission of pulsars
The available data on isolated X-ray pulsars, their wind nebulae, and the
supernova remnants which are connected to some of these sources are analyzed.
It is shown that electric fields of neutron stars tear off charged particles
from the surface of neutron star and trigger the acceleration of particles. The
charged particles are accelerated mainly in the field of magneto-dipole
radiation wave. Power and energy spectra of the charged particles depend on the
strength of the magneto-dipole radiation. Therefore, the X-ray radiation is
strongly dependent on the rate of rotational energy loss and weakly dependent
on the electric field intensity. Coulomb interaction between the charged
particles is the main factor for the energy loss and the X-ray spectra of the
charged particles.Comment: minor correction on table format, 20 pages (4 figures, 1 table),
submitted to International Journal of Modern Physics
Label-free electrochemical detection of human methyltransferase from tumors
The role of abnormal DNA methyltransferase activity in the development and progression of cancer is an essential and rapidly growing area of research, both for improved diagnosis and treatment. However, current technologies for the assessment of methyltransferase activity, particularly from crude tumor samples, limit this work because they rely on radioactivity or fluorescence and require bulky instrumentation. Here, we report an electrochemical platform that overcomes these limitations for the label-free detection of human DNA(cytosine-5)-methyltransferase1 (DNMT1) methyltransferase activity, enabling measurements from crude cultured colorectal cancer cell lysates (HCT116) and biopsied tumor tissues. Our multiplexed detection system involving patterning and detection from a secondary electrode array combines low-density DNA monolayer patterning and electrocatalytically amplified DNA charge transport chemistry to measure selectively and sensitively DNMT1 activity within these complex and congested cellular samples. Based on differences in DNMT1 activity measured with this assay, we distinguish colorectal tumor tissue from healthy adjacent tissue, illustrating the effectiveness of this two-electrode platform for clinical applications
DA495 - an aging pulsar wind nebula
We present a radio continuum study of the pulsar wind nebula (PWN) DA 495
(G65.7+1.2), including images of total intensity and linear polarization from
408 to 10550 MHz based on the Canadian Galactic Plane Survey and observations
with the Effelsberg 100-m Radio Telescope. Removal of flux density
contributions from a superimposed \ion{H}{2} region and from compact
extragalactic sources reveals a break in the spectrum of DA 495 at 1.3 GHz,
with a spectral index below the break and
above it (). The
spectral break is more than three times lower in frequency than the lowest
break detected in any other PWN. The break in the spectrum is likely the result
of synchrotron cooling, and DA 495, at an age of 20,000 yr, may have
evolved from an object similar to the Vela X nebula, with a similarly energetic
pulsar. We find a magnetic field of 1.3 mG inside the nebula. After
correcting for the resulting high internal rotation measure, the magnetic field
structure is quite simple, resembling the inner part of a dipole field
projected onto the plane of the sky, although a toroidal component is likely
also present. The dipole field axis, which should be parallel to the spin axis
of the putative pulsar, lies at an angle of {\sim}50\degr east of the North
Celestial Pole and is pointing away from us towards the south-west. The upper
limit for the radio surface brightness of any shell-type supernova remnant
emission around DA 495 is OAWatt
m Hz sr (assuming a radio spectral index of ), lower than the faintest shell-type remnant known to date.Comment: 25 pages, accepted by Ap
An upper limit on anomalous dust emission at 31 GHz in the diffuse cloud [LPH96]201.663+1.643
[LPH96]201.663+1.643, a diffuse H{\sc ii} region, has been reported to be a
candidate for emission from rapidly spinning dust grains. Here we present
Cosmic Background Imager (CBI) observations at 26-36 GHz that show no evidence
for significant anomalous emission. The spectral index within the CBI band, and
between CBI and Effelsberg data at 1.4/2.7 GHz, is consistent with optically
thin free-free emission. The best-fitting temperature spectral index from 2.7
to 31 GHz, , is close to the theoretical value,
for K. We place an upper limit of 24% ~ (2\sigma)
for excess emission at 31 GHz as seen in a 6\arcmin FWHM beam. Current
spinning dust models are not a good fit to the spectrum of LPH96. No polarized
emission is detected in the CBI data with an upper limit of 2% on the
polarization fraction.Comment: 5 pages, 3 figures, submitted to ApJ
G328.4+0.2 : A large and luminous Crab-like supernova remnant
We report on radio continuum and HI observations of the radio source
G328.4+0.2 using the Australia Telescope Compact Array. Our results confirm
G328.4+0.2 to be a filled-center nebula with no surrounding shell, showing
significant linear polarization and an almost flat spectral index. These
results lead us to conclude that G328.4+0.2 is a Crab-like, or ``plerionic'',
supernova remnant (SNR), presumably powered by an unseen central pulsar. HI
absorption towards G328.4+0.2 puts a lower limit on its distance of 17.4 +/-
0.9 kpc, making it the largest (D=25 pc) and most luminous (L_R = 3e35 erg/s)
Crab-like SNR in the Galaxy. We infer G328.4+0.2 to be significantly older than
the Crab Nebula, but powered by a pulsar which is fast spinning (P<20 ms) and
which has a comparatively low magnetic field (B<1e12 G). We propose G328.4+0.2,
G74.9+1.2 and N157B as a distinct group of large-diameter, high-luminosity
Crab-like SNRs, all powered by fast-spinning low-field pulsars.Comment: 7 pages, 3 embedded EPS figures, uses emulateapj.sty. Accepted to
ApJ. Abstract corrected so that distance is now in kpc, not pc
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