Prolonged abstinence from developmental cocaine exposure dysregulates BDNF and its signaling network in the medial prefrontal cortex of adult rats.

Although evidence exists that chronic cocaine exposure during adulthood is associated with changes in BDNF expression, whether and how cocaine exposure during adolescence modulates BDNF is still unknown. To address this issue, we exposed rats to repeated cocaine injections from post-natal day (PD) 28 to PD 42, a period that roughly approximates adolescence in humans, and we carried out a detailed analysis of the BDNF system in the medial prefrontal cortex (mPFC) of rats sacrificed 3 d (PD 45) and 48 d (PD 90) after the last cocaine treatment. We found that developmental exposure to cocaine altered transcriptional and translational mechanisms governing neurotrophin expression. Total BDNF mRNA levels, in fact, were enhanced in the mPFC of PD 90 rats exposed to cocaine in adolescence, an effect sustained by changes in BDNF exon IV through the transcription factors CaRF and NF-kB. While a profound reduction of specific BDNF-related miRNAs (let7d, miR124 and miR132) may contribute to explaining the increased proBDNF levels, the up-regulation of the extracellular proteases tPA is indicative of increased processing leading to higher levels of released mBDNF. These changes were associated with increased activation of the trkB-Akt pathway resulting in enhanced pmTOR and pS6 kinase, which ultimately produced an up-regulation of Arc and a consequent reduction of GluA1 expression in the mPFC of PD 90 cocaine-treated rats. These findings demonstrate that developmental exposure to cocaine dynamically dysregulates BDNF and its signaling network in the mPFC of adult rats, providing novel mechanisms that may contribute to cocaine-induced changes in synaptic plasticity

Platinum, Palladium and Rhodium deposition to the Prunus laurus cerasus leaf surface as an indicator of the vehicular traffic pollution in the city of Varese area. An easy and reliable method to detect PGEs released from automobile catalytic converters

Background, aim, and scope The widespread use of some Platinum Group Elements (PGE) as catalysts to minimize emission of pollutants from combustion engines produced a constantly growing increase of the concentration of these elements in the environment; their potential toxicological properties explain the increasing interest in routine easy monitoring. We have found that leaves of Prunus laurus cerasus are efficient collectors of particulate with a dimension < 60-80 \u3bcm, and a simple and reliable procedure was developed to reveal traces of platinum, palladium and rhodium released from automotive catalysts. The analysis of the dust deposited on the foliage is a direct indicator of traffic pollution. Materials and methods Leaves of prunus laurus cerasus were washed by sonication in a mixture of water and 2 propanol and the washings, to be discarded, were separated by centrifugation to yield typically 0.05-1.2 g of dust that, after mineralization, were directly submitted to Atomic Absorption analysis. Results Comparison of the 2007 and 2004-5 results showed a dramatic reduction of the platinum levels and revealed that palladium is now the main component of this traffic related pollution. Discussion The results are consistent with the increasing diffusion of cars with a diesel engine whose catalysts are made up of Pt and/or Pd alone, and gives a significant insight into the recent evolution in catalyst design that replaces platinum for palladium. Conclusion The proposed analytical procedure is simple, with short preparation times, and greatly reduces matrix effects so that atomic absorption spectroscopy can easily detect the three noble metals at the ng/g level in the dust. Recommendation and perspectives The results clearly show that Pd concentrations have increased over time, and must be cause of concern

Managing the commons in the knowledge economy

The work leading to this publication has received funding from the European Union’s Seventh Framework Programme (FP7/2007- 2013) under grant agreement n° 610349.This report presents an in-depth analysis of the concept of common goods and of possible political and management variation in the context of a knowledge-based economy. The research presents an initial critical review of the literature together with a concrete analysis of the development of the commons and common goods.The report will be organised in three sections. In the first, entitled "From the theory of public goods to the new political economy of the commons" we will see how, for Ostrom's new theory of the commons, what remains as a central element defining common goods is the particular nature of certain goods, in continuity with the ahistorical and static approach to classification of goods (private, public, common, belonging to a club) driven by neo-classical inspired economic theory.In the second section we will develop the approach of Common in the singular drawn up with the contribution of numerous studies in the theoretical framework of cognitive capitalism.The third will deal with the historic and empirical analysis of the origin, sense and principal stakes at play in the dynamics of the common, starting from the key role of the transformations of labour at the foundation of a knowledge-based economy.Throughout this journey, in the three sections different crucial aspects relating to the forms of regulation open to guarantee the sustainability of the commons and promote its development as a new central form of economic and social organisation will be faced systematically.This research offers an exhaustive theoretical framework, tackling all the conceptual and historical issues on the evolution of the theory of common goods. At the same time however, it offers practical and regulative examples of models of self-governance of commons, in the context of the knowledge-based economy. This analysis offers the D-CENT project possible models of democratic management of resources and common infrastructures that are at the base of the experience of shared democracy in Spain, Iceland and Finland, with the aim of achieving middle and long-term sustainability. Specifically speaking, the analysis submitted here reports: (1) research into the market of identity and the opposing claim of social data as digital common goods and the need for public and common infrastructures of information and communication not based on the logic of the market and surveillance (D3.3); (2) models to implement a commons currency of the common that can support the activities of social movements and productive communities (D3.5); (3) the final report (D1.3) on models of sustainability and the general impact of this project.Many of the examples proposed here, from the re-municipalisation of water, the self-management of cultural spaces to the free software and makers’ movement, illustrate collective practices that establish new spaces, institutions or norms of participative and democratic sharing. These examples represent practices of re-appropriation and management of the common, new practices of labour, creation and production based on collaboration and sharing.Moreover, from the concrete experiences analysed here, the idea emerges that the concept of common goods can constitute a concrete alternative, and that includes on a legal footing (Rodotà, 2011). Therefore the common is the product of a social and institutional structure that demonstrates forms of governing and social co-operation that guarantee its production, reproduction and spread. The new institutions of the common that emerge from these constituent practices constitute a general principle of self-governance of society and self-organisation of socialproduction, proposing a new division between common, public and private.Obviously, the success of these new practices is a complex process that must rely on institutions which accord and guarantee reproduction over time and space of the commons and common goods: ways of management based on self-governance and collaborative economics; relationships of exchange based on reciprocity and gratuitousness; legal regimes that, like the invention of copyleft for free software, guarantee the accumulation of a stock of common-pool resources (CPR); distribution norms that permit the active involvement of the commoners in the development of the commons, guaranteeing a basic income, for example.In this context, it becomes more and more essential and urgent to define the terms of an alternative model of regulating a knowledge-based society and economy at the centre of which the logic of the commons would perform an essential role

Chronic treatment with fluoxetine up-regulates cellular BDNF mRNA expression in rat dopaminergic regions.

During the last few years several studies have highlighted the possibility that major depression can be characterized by a general reduction in brain plasticity and an increased vulnerability under challenging situations. Such dysfunction may be the consequence of reduced expression and function of proteins important for neuroplasticity such as brain-derived neurotrophic factor (BDNF). On this basis, by using a sensitive non-radioactive in-situ hybridization, we evaluated the effects of a chronic treatment with fluoxetine on BDNF expression within rat dopaminergic regions. In fact, besides the well-established role of the hippocampus, increasing evidence indicates that other brain regions may be involved in the pathophysiology of depression and consequently be relevant for the therapeutic action of antidepressant drugs. Our results indicate that 3 wk of fluoxetine administration up-regulates BDNF mRNA levels selectively within structures belonging to the meso-cortico-limbic pathway. The expression of the neurotrophin is significantly increased in the ventral tegmental area, prefrontal cortex, and shell region of the nucleus accumbens, whereas no changes were detected in the substantia nigra and striatum. Moreover, in agreement with previous studies, fluoxetine increased BDNF mRNA levels in the hippocampus, an effect that was limited to the cell bodies without any change in its dendritic targeting. These data show that chronic treatment with fluoxetine increases BDNF gene expression not only in limbic areas but also in dopaminergic regions, suggesting that such an effect may contribute to improve the function of the dopaminergic system in depressed subjects

Particle Measurement Programme (PMP) Heavy-Duty (HD) Inter-laboratory Exercise - Validation Exercise Tests at JRC (Phase A: Feb. '08 and Phase B: June '08)

This document reports the results of the validation exercise during the PMP Heavy-Duty inter-laboratory exercise in Feb. ¿08 (Phase A) and June ¿08 (Phase B) conducted at the Vehicles Emissions Laboratory (VELA-5) in the Transport and Air Quality Unit of the European Commission¿s Joint Research Centre (JRC, Ispra). This report presents the results of the work undertaken on an IVECO Cursor 8 Heavy-Duty engine equipped with a Continuous Regenerating Trap (CRT), i.e. the Golden Engine. The tests included European and World Harmonized cycles following a strict protocol. Mass and number measurements were conducted simultaneously at the full flow and the partial flow sampling systems.JRC.H.4-Transport and air qualit

Short-term abstinence from cocaine self-administration, but not passive cocaine infusion, elevates αCaMKII autophosphorylation in the rat nucleus accumbens and medial prefrontal cortex

Increases in alpha calcium/calmodulin-dependent protein kinase type II ( α CaMKII) activity in the nucleus accumbens shell has been proposed as a core component in the motivation to self-administer cocaine and in priming-induced drug-seeking. Since cocaine withdrawal promotes drug-seeking, we hypothesized that abstinence from cocaine self-administration should enhance α CaMKII as well. We found that short-term abstinence from contingent, but not non-contingent, cocaine i.v. self-administration (2 h/d for 14 d; 0.25 mg/0.1 ml, 6s infusion) elevates α CaMKII autophosphorylation, but not the kinase expression, in a dynamic, time- and brain region-dependent manner. Increased α CaMKII autophosphorylation in the nucleus accumbens (NAc) and medial prefrontal cortex (mPFC), but not dorsolateral striatum (dlS), was found 24 h, but not immediately, after the last cocaine self-administration session. Notably, in the mPFC, but not NAc and dlS, α CaMKII autophosphorylation was still enhanced 7 d later. The persistent enhancement in the mPFC of abstinent rats may represent a previously unappreciated contribution to initial incubation of cocaine-seeking

miRNA expression is increased in serum from patients with semantic variant primary progressive aphasia

Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28