Fine-needle Aspiration Biopsy For Breast Lesions: A Comparison Between Two Devices For Obtaining Cytological Samples

Context and Objective: Fine-needle aspiration biopsy has been accepted worldwide for breast lesions. However, some questions remain, including the appropriateness of the puncture method. The objective of this work was to compare aspirates obtained by the auto-vacuum device and by the syringe pistol holder. Design and Setting: Randomized trial for validation of diagnostic method, at Hospital das Clinicas do Universidade Federal de Goiás and Hospital Araújo Jorge, Goiânio. Methods: 351 patients presenting breast lumps underwent fine-needle aspiration biopsy, either with the auto-vacuum device or the syringe pistol holder. A single cytopathologist analyzed all of the cytology slides. The rates of insufficient material, cellularity, cell distortion and background hemorrhage were evaluated. Results: The percentages of insufficient material were 16% and 22% (p = 0.18), for the auto-vacuum and pistol aspirates, respectively. Good cellularity was seen in 34% of autovacuum and 29% of pistol samples (p = 0.4). Cell distortion was seen in 31 and 26 cases, respectively (p = 07). Background hemorrhage occurred in 63 (35%) and 54 cases (31%) (p = 0.2), for auto-vacuum and pistol. The sensitivity was 88% and 86%; specificity 99% and 100%, positive predictive value 96% and and 100%, negative predictive value 96% and 95% and total occuracy 76% and 75% for the auto-vacuum and pistol, respectively. Conclusion: The results obtained from the two fine-needle aspiration biopsy methods were equivalent. Therefore, the auto-vacuum device is a good option for obtaining aspirates for cytology.1236271276Catania, S., Ciatto, S., Breast cytology: Instruments and technique (1992) Breast Cytology in Clinical Practice, pp. 11-60. , In: Ciatto S, editor. London: Martin DunitzDe Freitas Jr., R., Hamed, H., Fentiman, I., Fine needle aspiration cytology of palpable breast lesions (1992) Br J Clin Pract, 46 (3), pp. 187-190Franzen, S., Zajicek, J., Aspiration biopsy in diagnosis of palpable lesions of the breast. Critical review of 3479 consecutive biopsies Acta (1968) Radiol Ther Phys Biol, 7 (4), pp. 241-262Souza, J.A., Freitas Jr., R., Moreita, M.A.R., Philocreon, G.R., Acurácia da sensação tátil da PAAF no diagnóstico de less̄ocs mamárias (1995) Rev Bras Mastologia, 5 (3), pp. 7-10Freitas Júnior, R., Hamed, H., Millis, R.R., Tomas, N.M.A., Fentiman, I.S., Fine needle aspiration of breast lesions using auto-vacuum dispositive (1996) Rev Bras Mastologia, 6 (3), pp. 126-128de Freitas Júnior, R., Giraldo, P.C., Rerrori, O., Vieira-Matos, A.N., Tambascia, J.K., Fine needle aspiration biopsy of solid tumours by auto-vacuum system: A study in rats (1992) Eur J Surg Oncol, 18 (6), pp. 605-607Boccato, P., How to treat the aspirated material (1992) Breast Cytology in Clinical Practice, pp. 61-64. , In: Ciatto, S, editor London: Martin Dunitz(1987) International Union Against Cancer, , UICC. TNM classification of malignant tumors. Berlin: Springer-Verlag(1982) Am J Clin Pathol, 78 (6), pp. 806-816. , The World Health Organization Histological Typing of Breast Tumors - Second Edition. The World OrganizationGelabert, H.A., Hsiu, J.G., Mullen, J.T., Jaffe, A.H., D'Amato, N.A., Prospective evaluation of the rate of fine-needle aspiration biopsy in the diagnosis and management of patiencs with palpable solid breast lesions (1990) Am Surg, 56 (4), pp. 263-267Rouquayrol, M.Z., Epidemiologia e saúde (1988), Rio de Janeiro: Medsi - Editora CientíficaRoberts, J.C., Rainsbury, R.M., 'Tactile sensation: A new clinical sign during fine needle aspiration of breast lumps Ann (1994) R Coll Surg Eng, 76 (2), pp. 136-138Henderson, M.A., McCready, D.R., A simple technique for fine needle aspiration cytology (1994) J Am Coll Surg, 179 (4), pp. 471-473Sasaki, J., Izu, K., Automatic fine needle aspiration pistol specially designed for small tumors (1998) Acta Cytol, 42 (3), pp. 829-830Tao, L.C., Smith, J.W., Fine-needle aspiration biopsy using a newly-developed pencil-grip syringe holder (1999) Diagn Cytopathol, 20 (2), pp. 99-104Parel, J.J., Garrell, P.C., Smallwood, J.A., Fine needle aspiration cytology of breast masses: An evaluation of its accuracy and reasons for diagnostic failure (1987) Ann R Coll Surg Engl, 69 (4), pp. 156-159Abele, J., Stanley, M.W., Rollnis, S.D., Miller, T.R., What constitutes an adequate smear in fine-needle aspiration cytology of the breast? (1998) Cancer, 84 (1), pp. 57-61Zajdela, A., Ghossein, N.A., Pilleron, J.P., Ennuyer, A., The value of aspiration cytology in the diagnosis of breast cancer: Experience at the Fondation Curie (1975) Cancer, 35 (2), pp. 499-506Layfield, L.J., Dodd, L.G., Cytologically low grade malignancies: An important interpretative pitfall responsible for false negative diagnoses in fine-needle aspiration of the breast (1996) Diagn Cytopathol, 15 (3), pp. 250-259Feichter, G.E., Haberthür, F., Gobat, S., Dalquen, P., Breast cytology. 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Spatial distribution of bed variables, animal welfare indicators, and milk production in a closed compost-bedded pack barn with a negative tunnel ventilation system

This research aimed to characterize, evaluate and compare the spatial distribution of the leading bed variables, animal welfare indicators, and milk production in a closed compost-bedded pack barn (CBP) with a negative tunnel ventilation system, for summer and winter periods. The study was carried out in a CBP located in the Zona da Mata region, Minas Gerais, Brazil. The geostatistical modeling technique evaluated the variables of temper ature, moisture content, and pH (on the surface and depth of 0.20m) across the length of the bed. Bed samples were characterized for carbon (C), nitrogen (N), and C:N ratio. Cows housed in the CBP were assessed for locomotion and hygiene scores and average milk production. To evaluate the thermoregulation of the cows, the respiratory rate (RR) and surface temperature (ST) were measured. Geostatistical analysis showed spatial dependence and the non-uniformity of the spatial distribution of bed variables. The worst levels of bed tem perature and moisture were found in the regions close to the evaporative cooling plate, surrounding the feeding alley, and in the region with the highest cow stocking. The C:N ratio, obtained in both climatic seasons of the year, remained outside the recommended range for ideal composting. During the summer and winter, the bed variables’ values suggest that the material was below levels for optimal composting; however, the aerated inner layer was biologically active. The high animal density significantly impacted the worsening of the bed moisture content and internal temperature. In general, dairy cows showed adequate hygiene (score of 1 and 2) and locomotion (score of 0 and 1) scores for the two climatic seasons evaluated, indicating good welfare conditions. In relation to RR and ST, the summer period presented less favorable environmental conditions. During winter, the average milk production was 28.1 ± 7.2 kg day-1, and during summer, it was 26.9 ± 6.7 kg day-1

Effect of environmental temperature during the of brooding period on growing period of pullets viscera and tibia

ArticlePoultry production in subtropical and tropical regions faces many problems, one of which is the high air temperature causing thermal stress, particularly dangerous in high-producing birds. Thus, the negative effects caused by heat stress (HS) must be managed. The objective of this study was to evaluate the effects of four different levels of HS in viscera and tibia of pullets. A total of 648 chicks (Lohmann LSL Lite) were used in this study in two different phases. The pre-experimental phase (PEP) was from day 1 through 6 weeks of age. The birds were reared with three different environmental temperatures: thermal comfort, hot and cold. The experimental phase (EP) was conducted from the 7th to the 17th week. Pullets from each thermal environment of the PEP were submitted to: 20 °C, 25 °C, 30 °C, 35 °C. At the end of the 17th week of age 120 pullets were euthanatized and the organs, heart, liver, spleen and gizzard were weighed, as also their tibias. Effects of PEP, and its interaction with EP, were not significant (P < 0.05) for viscera and tibia weight. However, a significant increase (P < 0.05) in heart weight with the decrease of the environmental temperature was observed, being the pullets subject to 20ºC and 25 °C with the heaviest weights. For the liver, pullets subject to the 35 °C had the lowest weight and were different (P < 0.05) from the other three treatments. For gizzard, the difference (P < 0.05) was between the treatments 20ºC and 35 °C. These results indicate that brooding temperatures tested during the first 6 weeks of life did not affect the viscera and bone weight during the growing phase

Ligand-Free Palladium-Catalyzed Oxyarylation of Dihydronaphthal­enes and Chromenequinone with o-Iodophenols and 3-Iodolawsone in PEG-400: An Efficient Synthesis of 5-Carbapterocarpans and Pterocarpanquinones

Dihydronaphthalenes were oxyarylated with o-iodophenols, in PEG-400 at 140 or 170 °C, leading regio- and stereoselectively to 5-carbapterocarpans. By using Pd(OAc)2 (5–10 mol%) as precatalyst and Ag2CO3 (1.1 equiv) as base (conditions A), products were obtained in good to excellent chemical yields, in 5–30 minutes, irrespective of the pattern of substitution the starting materials. Alternatively, when p-hydroxyacetophenone oxime derived palladacycle (1 mol%) was used as precatalyst, and dicyclohexylamine (2 equiv) was used as base (silver-free, conditions B), the corresponding adducts were obtained in moderate to good yields, in 0.5 to 4 hours. Finally, the oxyarylation of dihydronaphthalenes­ and chromenquinone with o-iodophenols and 3-iodolawsone in PEG-400 under conditions A led regio- and stereoselectively to the formation of carbapterocarpanquinones and pterocarpanquinones in moderate yield.Financial support from Brazilian agencies CAPES-DGU (Project 200/09), CNPq, FAPERJ and UFRJ are acknowledged. Spanish MICINN (Projects PHB2008-0037-PC, CTQ2007-62771/BQU, CTQ2010-20387, Consolider INGENIO 2010 CSD2007-00006), FEDER, Generalitat Valenciana (Project PROMETEO/2009/038), and the University of Alicante are acknowledged

Tentativas De Suicídio E Suicídios Na Atenção Pré-hospitalar

To characterize suicide attempts and suicides in a city in Northwestern Parana State, attended in the pre-hospital care. Methods: Descriptive, cross-sectional study, performed with pre-hospital care records to 257 victims of suicide and suicide attempt, in the period in 2005 to 2012, through two-dimensional analysis by chi-square. Results: There were 180 attempted suicides and 76 suicides. The age ranged 13-93 years, mean 33.5 ± 15.1 years and most prevalent in young male population. The events concentrated on the months of spring and summer, in the urban area. The use of biting object was the most used method, followed by poisoning and precipitation high places. Conclusion: To know the characteristics, methods and aggravating factors provide subsidies for the implementation of prevention measures. © 2016, Editora Cientifica Nacional Ltda. All rights reserved.65323123

Supersymmetry Without Prejudice

We begin an exploration of the physics associated with the general CP-conserving MSSM with Minimal Flavor Violation, the pMSSM. The 19 soft SUSY breaking parameters in this scenario are chosen so as to satisfy all existing experimental and theoretical constraints assuming that the WIMP is a conventional thermal relic, ie, the lightest neutralino. We scan this parameter space twice using both flat and log priors for the soft SUSY breaking mass parameters and compare the results which yield similar conclusions. Detailed constraints from both LEP and the Tevatron searches play a particularly important role in obtaining our final model samples. We find that the pMSSM leads to a much broader set of predictions for the properties of the SUSY partners as well as for a number of experimental observables than those found in any of the conventional SUSY breaking scenarios such as mSUGRA. This set of models can easily lead to atypical expectations for SUSY signals at the LHC.Comment: 61 pages, 24 figs. Refs., figs, and text added, typos fixed; This version has reduced/bitmapped figs. For a version with better figs please go to http://www.slac.stanford.edu/~rizz