12 research outputs found
Variación cuantitativa y cualitativa de la composición en ácidos grasos de Crypthecodinium cohnii en condiciones de supresión de nitrógeno
Get PDFCrypthecodinium cohnii is a heterotrophic dinoflagellate that can achieve a lipid content greater than 20% on dry weight. DHA can represent up to 40% of the total fatty acids. Our objective was to investigate the variation in fatty acid content when growth conditions are optimal compared with when there is limited growth, due to the absence of nitrogen in the media. Nitrogen-limited growth conditions caused the cells to stop dividing and accumulate lipids, principally as docohexanoic acid (DHA). Oxygen availability in the culture favoured DHA accumulation. In the cultures without nitrogen and with oxygen availability there was a lipid cell content 3,18 times higher than in the control condition (with nitrogen and air), and 2,25 higher than those without nitrogen and air. C. cohnii can be used as an optimal DHA source for the production of phytodiets in aquaculture.Crypthecodinium cohnii, es un dinoflagelado marino heterotrófico estricto, que puede alcanzar contenidos en lípidos superiores al 40% de su peso seco, representando el DHA más del 40% del conjunto total de ácidos grasos. El objetivo del presente trabajo fue el de establecer el perfil de variación de los ácidos grasos en condiciones de óptima disponibilidad de nutrientes y de supresión de nitrógeno. Los resultados muestran que en condiciones de supresión de nitrógeno las células dejan de dividirse y empiezan a acumular lípidos, especialmente ácido docosahexaenoico (DHA). La disponibilidad de oxígeno en el medio también favorece este proceso. En los cultivos sin nitrógeno y con disponibilidad de oxígeno se alcanzó un contenido celular de ácidos grasos 3,18 veces superior al alcanzado en la condición control (con nitrógeno y aire) y 2,25 al observado en los cultivos sin nitrógeno no burbujeados. Crypthecodinium cohnii puede constituir una óptima fuente de DHA para la elaboración de fitodietas en acuicultura
Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
Get PDFIn the present work the characterization of different strains of
Dunaliella salina from established cell culture collections and
various isolates from solar saltworks located in the Canary Island, as
well as one mutant, was carried out in order to assess the existence of
intraspecific differences and to determine the potential productivity
of each one. Morphological characteristics such as cellular size and
cellular complexity and parameters linked to cellular physiology, such
as pigment content or cellular growth rate, were determined by means of
traditional techniques as well as flow cytometry. Results showed a high
morphological and physiological intraspecific variability among the
studied strains. Results suggest that the application of the lipidic
dye Nile red allowed the development of a cytometric method faster than
the traditional techniques to select carotenoid hyperproducing strains
of microalgae
Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
Get PDFIn the present work the characterization of different strains of
Dunaliella salina from established cell culture collections and
various isolates from solar saltworks located in the Canary Island, as
well as one mutant, was carried out in order to assess the existence of
intraspecific differences and to determine the potential productivity
of each one. Morphological characteristics such as cellular size and
cellular complexity and parameters linked to cellular physiology, such
as pigment content or cellular growth rate, were determined by means of
traditional techniques as well as flow cytometry. Results showed a high
morphological and physiological intraspecific variability among the
studied strains. Results suggest that the application of the lipidic
dye Nile red allowed the development of a cytometric method faster than
the traditional techniques to select carotenoid hyperproducing strains
of microalgae
Labelling in Mediterranean agroforestry landscapes: a Delphi study on relevant sustainability indicators
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Donnan-Potential Sensors Based on Zirconia-Modified Nafion Membranes Treated under Different Conditions for the Determination of Amino Acids with Several Nitrogen-Containing Groups
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