EFFECT OF A SHORT PERIOD WHOLE BODY VIBRATION WITH 10 HZ ON BLOOD BIOMARKERS IN WISTAR RATS

Background: Exposure to whole body vibration exercises (WBVE), besides some biological effects, causes alterations in the concentration of some blood biomarkers. The aim of this study is to evaluate the action of vibration (10 Hz) of WBVE on the concentration of blood biomarkers in Wistar rats. Materials and Methods: Wistar rats were divided in 2 groups. The experimental group (EG) was subjected to vibrations of 10Hz (one min per day, one week, total time of seven min), while the control group (CG) has not experienced vibration. Samples of whole blood were drawn for biochemical analysis of the concentration of total cholesterol, triglycerides, HDL, LDL, VLDL, glucose, CPK, albumin, alkaline phosphates, TGP, TGO, γGT, lipase, amylase, urea and creatinine. Results: White blood cell count and a platelet-hemogram were also performed. Significant (

Influence of an aqueous extract of <i>Coriandrum sativum</i>; on the in vivo labeling of blood constituents with technetium-99m and their association with vibration generated in oscillating platform in the biodistribution of Na99mTcO4 radiopharmaceutical and in some concentration of biomarkers in Wistar rats

Coriandrum sativum, conhecido popularmente como coentro, é um vegetal usado na alimentação humana. Também é utilizado como planta medicinal para tratamento de diabetes, complicações gastrintestinais, e como um antiedêmico, antisséptico e emenagogo. Em investigações acerca dos efeitos do extrato de plantas, é importante a determinação de alguns parâmetros físico-químicos. Diversos modelos experimentais têm sido usados, inclusive com o emprego de radionuclídeos. Em procedimentos da Medicina Nuclear que auxiliam o diagnóstico de doenças, o tecnécio-99m (99mTc) é o radionuclídeo mais utilizado. Hemácias marcadas com 99mTc estão entre as várias estruturas celulares que podem ser marcadas com este radionuclídeo e usadas como radiofármaco. Para a marcação com 99mTc é necessária a presença de um agente redutor, e o mais utilizado é o cloreto estanoso (SnCl2). As terapias com drogas e condições de dieta além de doenças podem alterar a marcação de constituintes sanguíneos, bem como a biodistribuição de diferentes radiofármacos. A exposição às vibrações geradas por plataforma oscilatória produz exercícios de corpo inteiro. O objetivo deste estudo foi caracterizar a preparação de um extrato do Coriandrum sativum, através de parâmetros físico-químicos, verificar os efeitos desse produto natural na radiomarcação de constituintes sanguíneos e em associação à vibração gerada pela plataforma na biodistribuição de Na99mTcO4 e na concentração de alguns biomarcadores. O extrato de coentro teve a o pico de absorbância em 480 nm. O extrato de coentro foi inversamente correlacionado com a concentração na condutividade elétrica. Foi encontrado o maior valor de pH na menor concentração do extrato (0,5 mg/mL). Não houve uma alteração significativa na marcação de constituintes sanguíneos com 99mTc. E a associação do extrato de coentro e vibração gerada por plataforma com frequência de 12 Hz teve efeito no baço, como observado na fixação do radiofármaco nesse órgão e ação em alguns órgãos alternando a concentração de alguns biomarcadores. Em conclusão, parâmetros físico-químicos podem ser úteis para caracterizar o extrato estudado. Provavelmente, as propriedades redox associadas com substâncias desse extrato podem ser os responsáveis pela ausência do efeito na radiomarcação de constituintes sanguíneos. A determinação da captação do Na99mTcO4 em diferentes órgãos permite verificar que o extrato de coentro sozinho não foi capaz de interferir na biodistribuição do radiofármaco. Contudo o tratamento de animais com vibração gerada pela plataforma alterou significativamente a fixação do pertecnetato de sódio no baço e a concentração do colesterol, triglicerídeo, CK e bilirrubina.Coriandrum sativum, popularly known as coriander, is a vegetable used in human alimentation. It is also used as a medicinal plant for the treatment of diabetes, gastrointestinal complications, and as an antiedemic, antiseptic and emenagogue. In the investigations about the effects of a plant extract, it is important to determine some of its physicochemical parameters. Several experimental models have been used, including the use of radionuclides. In Nuclear Medicine procedures that assist the diagnosis of diseases, technetium-99m (99mTc) is the most used radionuclide. Red blood cells labeled with 99mTc are among the various cellular structures that can be labeled with this radionuclide and used as a radiopharmaceutical. The labeling process involving 99mTc requires the presence of a reducing agent and the most used for this purpose is the stannous chloride (SnCl2). The drugs therapy, diet conditions and diseases can alter the labeling of blood constituents, as well the biodistribution of several radiopharmaceuticals. The exposure to vibrations generated in oscillating platform produces whole body vibration (WBV) exercise. The aim of this study was to characterize the preparation of an extract of Coriandrum sativum, through physicochemical parameters, verify the effects of this natural product in radiolabeling of blood constituents and in association with vibration generated by platform on the Na99mTcO4 biodistribution, and in concentrations of some biomarkers. The extract of coriander had a pick absorbance at 480 nm. The coriander extract was inversely correlated with the concentration in electric conductivity. Was founded the highest value of pH at the lower concentration of the extract (0.5 mg/mL). There was no significant alteration on the labeling of blood constituents with 99mTc. The association between the coriander extract and vibration generated in platform with frequency of 12 Hz had effect in spleen, as observed in the fixation of the radiopharmaceutical in the organ and action in some organs altering the concentration of some biomarkers. In conclusion, physicochemical parameters can be useful to characterize the studied extract. Probably, the redox properties associated with the substances of this extract could be responsible by the absence of effect on the radiolabeling of blood constituents. The determination of the uptake of the radiopharmaceutical sodium pertechnetate in different organs permits to verify that extract of coriander alone was not capable in interfering on the biodistribution of the radiopharmaceutical. However, the treatment of the animals with vibration generated in the platform alters significantly the fixation of the sodium pertechnetate in the spleen and the concentrations of Cholesterol, triglyceride, CK and bilirubin

Effects of Mirror Therapy on the Lower Limb Functionality Hemiparesis after Stroke

Abstract Introduction: Mental exercise using the mirror therapy (MT) improves the retention of newly acquired skills and the performance of sequential motor skills in subjects with post-stroke hemiparesis. Objectives: The study aimed to analyze the mirror therapy effect on the range of motion and the lower limb functionality in poststroke hemiparesis subjects. Materials and Methods: Eleven participants with hemiparesis in the lower limb were subjected to the 10 sessions of a MT protocol. The interventions were three times per week per 30 minutes each day. Evaluation of active and passive ankle goniometry (dorsiflexion and eversion movements); Ascent and Descent Ladder Rate (ADLR); Time Up and Go test (TUG test); EFEI scale; and FAAM scale were performed. The data were collected before and after the intervention using MT, and then statistically compared. Results: The MT improved significantly (p &lt; 0.01) the range of motion of the paretic lower limb both evaluated by active and passive ankle goniometry. An increase in the speed of gait and other functional tasks related to the paretic lower limbs were found through the TUG and ADLR tests. It also demonstrated a positive influence on the functionality of the paretic lower limb motor control through the analysis of the scores in the FAAM and EFEI scales. Conclusion: It is concluded that the MT therapy can help the patients with post-stroke hemiparesis in the improvement of several functions. Probably, the mirror therapy would aid in the repair of the injuries in the cortical areas