A Langmuir approach on monolayer interactions to investigate surface active peptides

The Langmuir Blodgett apparatus provides a versatile system for studying the interfacial properties of peptides and peptide-membrane interactions under controlled conditions. Using amphiphilic α-helical peptides to highlight studies undertaken, here we discuss the use of this system to provide information on the surface activity of peptides and describe the insights these studies give into biological functio

BENEFIT CAPTURE: EVIDENCE FROM A RIVER CORRIDOR BENEFIT COST EVALUATION

The concept of and evidence for benefit capture are explored in a benefit cost analysis of selected water quality and infrastructure improvements in the Muskingum River corridor of South Eastern Ohio. Property tax revenues and CVM bid functions are estimated and implications for benefit capture and further research are developed.Resource /Energy Economics and Policy,

Increased Rates of Prolonged Length of Stay, Readmissions, and Discharge to Care Facilities among Postoperative Patients with Disseminated Malignancy: Implications for Clinical Practice.

BackgroundThe impact of surgery on end of life care for patients with disseminated malignancy (DMa) is incompletely characterized. The purpose of this study was to evaluate postoperative outcomes impacting quality of care among DMa patients, specifically prolonged length of hospital stay, readmission, and disposition.MethodsThe American College of Surgeons National Surgical Quality Improvement Program (ACS-NSQIP) database was queried for years 2011-2012. DMa patients were matched to non-DMa patients with comparable clinical characteristics and operation types. Primary hepatic operations were excluded, leaving a final cohort of 17,972 DMa patients. The primary outcomes were analyzed using multivariate Cox regression models.ResultsDMa patients represented 2.1% of all ACS-NSQIP procedures during the study period. The most frequent operations were bowel resections (25.3%). Compared to non-DMa matched controls, DMa patients had higher rates of postoperative overall morbidity (24.4% vs. 18.7%, p<0.001), serious morbidity (14.9% vs. 12.0%, p<0.001), mortality (7.6% vs. 2.5%, p<0.001), prolonged length of stay (32.2% vs. 19.8%, p<0.001), readmission (15.7% vs. 9.6%, p<0.001), and discharges to facilities (16.2% vs. 12.9%, p<0.001). Subgroup analyses of patients by procedure type showed similar results. Importantly, DMa patients who did not experience any postoperative complication experienced significantly higher rates of prolonged length of stay (23.0% vs. 11.8%, p<0.001), readmissions (10.0% vs. 5.2%, p<0.001), discharges to a facility (13.2% vs. 9.5%, p<0.001), and 30-day mortality (4.7% vs. 0.8%, p<0.001) compared to matched non-DMa patients.ConclusionSurgical interventions among DMa patients are associated with poorer postoperative outcomes including greater postoperative complications, prolonged length of hospital stay, readmissions, disposition to facilities, and death compared to non-DMa patients. These data reinforce the importance of clarifying goals of care for DMa patients, especially when acute changes in health status potentially requiring surgery occur

A theoretical analysis of secondary structural characteristics of anticancer peptides

Here, cluster analysis showed that a database of 158 peptides formed 21 clusters based on net positive charge, hydrophobicity and amphiphilicity. In general these clusters showed similar median toxicities (p = 0.176) against eukaryotic cell lines and no single combination of these properties was found optimal for efficacy. The database contained 14 peptides, which showed selectivity for tumour cell lines only (ACPCT), 123 peptides with general toxicity to eukaryotic cells (ACPGT) and 21 inactive peptides (ACPI). Hydrophobic arc size analysis showed that there was no significant difference across the datasets. Even though there was no correlation there was no correlation observed, peptides with wide hydrophobic arcs (> 270°) appeared less toxic. Extended hydrophobic moment plot analysis predicted that over 50% of ACPCT and ACPGT peptides would be surface active, which led to the suggestion that amphiphilicity is a key driver of the membrane interactions for these peptides but probably plays a role in their efficacy rather than their selectivity. This analysis also predicted that only 14% of ACPCT peptides compared to 45% of ACPGT peptides were candidates for tilted peptide formation. This implies that those peptides with non-specific activity may have a tendency towards the utilisation of membrane disruptive structures such as tilt peptides which led to the suggestion that the absence of this structure may support cancer cell selectivity. However, these analyses predicted that ACPI peptides, which possess no anticancer activity, would also form surface active and tilted a-helices, clearly showing that other factors are involved in determining the efficacy and selectivity of ACPs

S18RS SGR No. 1 (TOPS)

A RESOLUTION To strongly urge the Louisiana State Legislature to FULLY fund the Taylor Opportunity Program for Students (TOPS) during the 2018 Legislative sessio

Program and Evaluation Plan: An Intervention to Increase Childhood Vaccination Rates in the Chapel Hill Area

Childhood vaccination rates in North Carolina are currently well below the rate needed to confer herd immunity. As a result, there were three measles outbreaks in the State in 2013. The goal of this intervention is to increase vaccination coverage for children aged 19-35 months through an educational program for parents who initially refuse vaccination in the Chapel Hill area. This paper provides the program and evaluation plans for the intervention. It opens with a systematic review of the literature, addressing similar interventions and their evaluation. The program plan addresses the development of the intervention; its goals and its implementation. The evaluation plan is a way to assess whether the intervention is being implemented as planned, as well as the efficacy of the intervention. The paper is a guide to the development, implementation and evaluation of the intervention, and provides an approach to dissemination.Master of Public Healt