369 research outputs found

    Centrifuge and real-time hybrid testing of tunnelling beneath piles and piled buildings

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    Tunnels are constructed increasingly close to existing buried structures, including pile foundations. This poses a serious concern, especially for tunnels built beneath piles. Current understanding of the global tunnel-soil-pile-building interaction effects is lacking, which leads to designs which may be overly conservative or the adoption of expensive measures to protect buildings. This paper presents outcomes from 24 geotechnical centrifuge tests that aim to investigate the salient mechanisms that govern piled building response to tunnelling. Centrifuge test data include greenfield tunnelling, pile loading, and tunnelling beneath single piles and piled frames, all within sand. The global tunnel-piled frame interaction scenario is investigated using a newly developed real-time hybrid testing technique, wherein a numerical model is used to simulate a building frame, a physical (centrifuge) model is used to replicate the tunnel-soil-foundation system and structural loads, and coupling of data between the numerical and physical models is achieved using a real-time load-control interface. The technique enables, for the first time, a realistic redistribution of pile loads (based on the superstructure characteristics) to be modelled in the centrifuge. The unique dataset is used to quantify the effects of several factors which have not previously been well defined, including the pile installation method, initial pile safety factor, and superstructure characteristics. In particular, results illustrate that pile settlement and failure mechanisms are highly dependent on the pre-tunnelling loads and the load redistribution that occurs between piles during tunnel volume loss, which are related to structure weight and stiffness. The paper also provides insight as to how pile capacity should be dealt with in a tunnel-pile interaction context.Engineering and Physical Sciences Research Council [grant number EP/K023020/1, 1296878, EP/N509620/1

    El derecho animal dentro del nuevo paradigma de derecho ambiental

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    This paper addresses the issue of Animal Law from a double perspective. Firstly, it reflects the legislative inconsistencies within the Argentine law regarding the legal status of the animal, which is mainly considered an object, on the one hand; and it exhibits the national and local jurisprudential development that recognizes the animal as a legal person -not human-, on the other hand. Secondly, this paper describes the characteristics of Animal Law that allows its incorporation as a chapter of the new paradigm of Environmental LawFil: Franza, Jorge A. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho de los Recursos Naturales y Protección del Medio Ambiente. Buenos Aires, ArgentinaDossier "Derecho ambiental y recursos naturales": El presente trabajo aborda el tema del Derecho Animal desde una perspectiva doble. En primer lugar, se reflejan, por un lado, las inconsistencias legislativas dentro del derecho argentino en cuanto al estatus jurídico del animal, al que se lo considera principalmente un objeto, y se muestra, por otro lado, el desarrollo jurisprudencial nacional y local que reconocen al animal como un sujeto de derecho no humano. En segundo lugar, se describen las características del Derecho Animal que permiten su incorporación como un capítulo del nuevo paradigma del Derecho Ambiental

    Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors

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    The SR proteins are essential metazoan pre-mRNA splicing factors that can also influence the selection of alternative 5' splice sites in a concentration-dependent manner. Their activity in alternative splicing in vitro is antagonized by members of the hnRNP A/B family of proteins. The opposite effects of members of these two families of antagonistic splicing factors in vitro and upon overexpression in vivo suggest that changes in their relative levels may be a natural mechanism for the regulation of alternative splicing in vivo. One prediction of this model is that the ratios of these antagonists should vary in different cell types and in other situations in which cellular or viral transcripts are differentially spliced. We raised monoclonal antibodies specific for SFS/ASF and used them to measure the abundance of SFS/ASF protein and its isoforms, its phosphorylation state in vivo and during splicing in vitro, and its association with the spliceosome. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in vivo in all cell types examined, and unphosphorylated protein was not detectable. Unphosphorylated recombinant SFS/ASF becomes rapidly phosphorylated under splicing conditions in HeLa cell extracts and associates stably with one or more exons of beta-globin pre-mRNA. This interaction appears to persist through the splicing reaction and SF2/ASF remains bound to spliced mRNA. We compared the distribution of SFS/ASF to that of its antagonist, hnRNP Al, in different rat tissues and in immortal and transformed cell lines. We found that the protein levels of these antagonistic splicing factors vary naturally over a very wide range, supporting the notion that changes in the ratio of these proteins can affect alternative splicing of a variety of pre-mRNAs in vivo
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