Ubiquinol affects the expression of genes involved in PPARα signalling and lipid metabolism without changes in methylation of CpG promoter islands in the liver of mice

Coenzyme Q10 is an essential cofactor in the respiratory chain and serves as a potent antioxidant in biological membranes. Recent studies in vitro and in vivo provide evidence that Coenzyme Q10 is involved in inflammatory processes and lipid metabolism via gene expression. To study these effects at the epigenomic level, C57BL6J mice were supplemented for one week with reduced Coenzyme Q10 (ubiquinol). Afterwards, gene expression signatures and DNA promoter methylation patterns of selected genes were analysed. Genome-wide transcript profiling in the liver identified 1112 up-regulated and 571 down-regulated transcripts as differentially regulated between ubiquinol-treated and control animals. Text mining and GeneOntology analysis revealed that the ”top 20” ubiquinol-regulated genes play a role in lipid metabolism and are functionally connected by the PPARα signalling pathway. With regard to the ubiquinol-induced changes in gene expression of about +3.14-fold (p≤0.05), +2.18-fold (p≤0.01), and −2.13-fold (p≤0.05) for ABCA1, ACYP1, and ACSL1 genes, respectively, hepatic DNA methylation analysis of 282 (sense orientation) and 271 (antisense) CpG units in the respective promoter islands revealed no significant effect of ubiquinol. In conclusion, ubiquinol affects the expression of genes involved in PPARα signalling and lipid metabolism without changing the promoter DNA methylation status in the liver of mice

Comparing the reliability of maize variety data from on-farm trials and experimental stations

Sortenempfehlungen basieren in der Regel auf regionalspezifischen Exaktversuchen. Dabei können die Wechselwirkungen zwischen Sorte und Umwelt sowie zwischen Sorte und Management zu steigenden Problemen bei der Sortenwahl führen. Eine ergänzende Möglichkeit sind daher dezentralisierte Sortenversuche in Landwirtschaftsbetrieben. Für die direkte Sortenwahl bieten diese On-Farm-Versuche eine hohe Praxisrelevanz, da die tatsächlichen Einflussgrößen im Betrieb getreuer abgebildet werden können, als es Exaktversuche vermögen, wenn sie nur an wenigen, zum Teil eingeschränkt repräsentativen Standorten durchgeführt werden. Andererseits ist die Aussagefähigkeit von On-Farm-Versuchen häufig durch Störgrößen und fehlende Feldwiederholungen begrenzt. Anhand von Maissortenversuchen mit sechs verschiedenen Sorten wurden auf vier Brandenburger Landwirtschaftsbetrieben sowie auf zwei Versuchsstationen die Sortenleistung in On-Farm- und Exaktversuchen auf Sandböden geprüft. An jedem Standort wurde die Varianz der Sortenrankings über die Jahre genutzt, um Informationen über die Konsistenz der Sortenleistung an jedem der Standorte zu gewinnen. Für den Trockenmasse-Ertrag zeigten die On-Farm-Versuche sowohl die höchste, als auch die niedrigste Konsistenz der Rankings. Einige Qualitätsparameter, wie die Nichtfaser-Kohlenhydrate (NFC) und der Stärkegehalt, zeigten in den On-Farm-Versuchen eine bessere Konsistenz des Sortenrankings über die Jahre als in beiden Exaktversuchen. Dies legt nahe, dass On-Farm-Versuche das Potenzial haben, die regionale Leistungsprüfung von Sorten zu unterstützen. DOI: 10.5073/JfK.2014.11.03, https://doi.org/10.5073/JfK.2014.11.03While current variety recommendations are based on replicated small-plot trials in a specific region for which the trial is thought to be representative, genotype × envi­ronment interactions, genotype × management interactions, and increasing weather fluctuations make it increasingly difficult to predict which variety will be best in a given environment. An additional approach is therefore to decentralize variety trials and place them on working farms. However, although on-farm trials offer potentially more relevance for direct variety selection on site, they are also likely to be subject to more noise and trial entries can often not be fully replicated. To evaluate the relative merit of on-farm trials vs. fully replicated trials conducted at experimental stations, we tested 6 maize varieties at four farms and at two stations in a region dominated by sandy soils. The variance of variety rankings over the years within each site was used as proxy to evaluate the consistency of variety information gained at each location. For dry matter yield, on-farm trials showed both the highest and the lowest consistency of variety ranking, with the consistency being intermediate at the experimental stations. For some quality parameters, namely non-fibre carbohydrate and starch content, the majority of on-farm trials showed more consistent variety ranking over the years than the more consistent of the two replicated trials. This suggests that in terms of year-on-year reliability of maize yield and quality, on-farm trials may have the potential to complement replicated variety trials. For both types of trials, however, there is also scope for decreasing technical sources of variation. DOI: 10.5073/JfK.2014.11.03, https://doi.org/10.5073/JfK.2014.11.0

Paraoxonase 1 polymorphism Q192R affects the pro-inflammatory cytokine TNF-alpha in healthy males

<p>Abstract</p> <p>Background</p> <p>Human paraoxonase 1 (PON1) is an HDL-associated enzyme with anti-oxidant/anti-inflammatory properties that has been suggested to play an important protective role against coronary heart diseases and underlying atherogenesis. The common <it>PON1 </it>Q192R polymorphism (<it>rs662</it>, A>G), a glutamine to arginine substitution at amino acid residue 192, has been analyzed in numerous association studies as a genetic marker for coronary heart diseases, however, with controversial results.</p> <p>Findings</p> <p>To get a better understanding about the pathophysiological function of PON1, we analyzed the relationships between the Q192R polymorphism, serum paraoxonase activity and serum biomarkers important for atherogenesis. Genotyping a cohort of 49 healthy German males for the Q192R polymorphism revealed an allele distribution of 0.74 and 0.26 for the Q and R allele, respectively, typical for Caucasian populations. Presence of the R192 allele was found to be associated with a significantly increased paraoxonase enzyme activity of 187.8 ± 11.4 U/l in comparison to the QQ192 genotype with 60.5 ± 4.9 U/l. No significant differences among the genotypes were found for blood pressure, asymmetric dimethylarginine, LDL, HDL, triglycerides, and cholesterol. As expected, MIP-2 alpha a cytokine rather not related to atherosclerosis is not affected by the <it>PON1 </it>polymorphism. In contrast to that, the pro-inflammatory cytokine TNF-alpha is enhanced in R192 carriers (163.8 ± 24.7 pg/ml vs 94.7 ± 3.2 pg/ml in QQ192 carriers).</p> <p>Conclusions</p> <p>Our findings support the hypothesis that the common <it>PON1 </it>R192 allele may be a genetic risk factor for atherogenesis by inducing chronic low-grade inflammation.</p

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The present guidelines describe the annotation of narrative phenomena on the clause level, using a combination of ideas and methods from linguistics and lit- erary studies. The main categories marking the discourse strategy “narration” in stretches of text have been narrowed down to mediacy, i. e. involving a narrator, and sequentiality of events. This document specifies how to define mediacy, and in turn determine whether a narrator is present, as well as how to identify events and their sequential ordering. Lastly, a functional layer annotation is proposed which allows researchers to compare different types of narrative instances. This offers a basis for investigating a potential narrative register which is said to be important for many kinds of register studies.Peer Reviewe

Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo

MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysaccharide (LPS) induces a reactive oxygen species-/NF-κB-dependent pathway which increases the expression of the anti-inflammatory miR-146a. We hypothesized that this induction could be modulated by the antioxidant ubiquinol-10. Preincubation of human monocytic THP-1 cells with ubiquinol-10 reduced the LPS-induced expression level of miR-146a to 78.9 ± 13.22%. In liver samples of mice injected with LPS, supplementation with ubiquinol-10 leads to a reduction of LPS-induced miR-146a expression to 78.12 ± 21.25%. From these consistent in vitro and in vivo data, we conclude that ubiquinol-10 may fine-tune the inflammatory response via moderate reduction of miR-146a expression

Grain legume yields are as stable as other spring crops in long-term experiments across northern Europe

Grain legumes produce high-quality protein for food and feed, and potentially contribute to sustainable cropping systems, but they are grown on only 1.5% of European arable land. Low temporal yield stability is one of the reasons held responsible for the low proportion of grain legumes, without sufficient quantitative evidence. The objective of this study was to compare the yield stability of grain legumes with other crop species in a northern European context and accounting for the effects of scale in the analysis and the data. To avoid aggregation biases in the yield data, we used data from long-term field experiments. The experiments included grain legumes (lupin, field pea, and faba bean), other broad-leaved crops, spring, and winter cereals. Experiments were conducted in the UK, Sweden, and Germany. To compare yield stability between grain legumes and other crops, we used a scale-adjusted yield stability indicator that accounts for the yield differences between crops following Taylor's Power Law. Here, we show that temporal yield instability of grain legumes (30%) was higher than that of autumn-sown cereals (19%), but lower than that of other spring-sown broad-leaved crops (35%), and only slightly greater than spring-sown cereals (27%). With the scale-adjusted yield stability indicator, we estimated 21% higher yield stability for grain legumes compared to a standard stability measure. These novel findings demonstrate that grain legume yields are as reliable as those of other spring-sown crops in major production systems of northern Europe, which could influence the current negative perception on grain legume cultivation. Initiatives are still needed to improve the crops agronomy to provide higher and more stable yields in future.Peer reviewe