Proteomics-based identification of salivary changes in patients with burning mouth syndrome

Burning mouth syndrome (BMS) is a chronic oral condition characterized by an intraoral burning sensation, taste alterations, and dry mouth sensations. Although a number of factors have been closely related to the appearance of the symptoms, including anxiety, depression, and sleep disturbances, the etiology of BMS remains unclear. Furthermore, currently no objective diagnostic tools exist, making its diagnosis challenging. Therefore, to contribute to the knowledge about BMS etiology and look for objective tools for its diagnosis, the present study was conducted. Thus, the aim of this study was to analyze the proteomic profile of the resting whole saliva of patients with BMS and age and sex-matched controls using two-dimensional electrophoresis. The results showed evidence of changes in saliva at the level of proteins related to important pathways such as stress (sAA), immune system (Ig), and inflammation (leukocyte elastase inhibitor). While some of our findings have been previously described others, such as the deregulation of the coiled-coin domain containing protein 25 in BMS, are presented here for the first time to our knowledge. Thus, saliva provides us with relevant information about BMS pathophysiology and could be considered a suitable biofluid for its study and/or diagnosi

Guía ilustrada de los procedimientos archivísticos de la documentación especializada de los establecimientos institucionales de salud del Instituto Salvadoreño de Bienestar Magisterial (ISBM).

El presente documento refleja una investigación relacionada a los procedimientos archivísticos que lleva a cabo la Sección de Gestión Documental y Archivos del Instituto Salvadoreño de Bienestar Magisterial (ISBM), específicamente en los documentos que se producen en los archivos especializados que genera de acuerdo a los servicios que brinda dicha Institución; al respecto se logró identificar una problemática en la transferencia de los documentos especializados hacia el Archivo Central, la cual podría ocasionar que no sea posible responder oportunamente y de acuerdo a los plazos establecidos en la Ley, a cualquier requerimiento de información, tanto de usuarios internos como externos, debido a la inadecuada organización de este tipo de documentos que generan las unidades productoras. En este sentido, se realizó una propuesta que vendría a solucionar esta problemática, la cual consiste en la elaboración de una guía ilustrada de los procedimientos archivísticos que se realizan a los documentos especializados; documento que servirá como una herramienta que facilite la comprensión de estos procedimientos, puesto que en ella se concentran única y específicamente los procedimientos que competen a las unidades productoras (Establecimientos Institucionales de Salud), y se detallan a manera de actividades en forma sencilla, clara y concisa, lo cual se espera sirva como otro instrumento con el que contará la institución para llevar a cabo los procedimientos archivísticos con éxito

Risk factors associated withStreptococcus suiscases on pigfarms in Spain

Background Streptococcus suis can cause meningitis, polyarthritis and acute death in piglets. However, the risk factors associated with S. suis infection remain incompletely understood. Therefore, a longitudinal study was carried out, in which six batches from two Spanish pig farms with S. suis problems were repeatedly examined to determine possible risk factors. Methods A prospective case–control study was conducted, and potential risk factors were evaluated using mixed-effects logistic regression models. The explanatory variables included: (a) concomitant pathogens; (b) biomarkers associated with stress, inflammation and oxidative status; (c) farm environmental factors; and (d) parity and S. suis presence in sows. Three models were built to study the effect of these variables, including two to assess the risk factors involved in the subsequent development of disease. Results Risk factors for S. suis-associated disease included porcine reproductive and respiratory syndrome virus co-infection at weaning (odds ratio [OR] = 6.69), sow parity (OR = 0.71), haptoglobin level before weaning (OR = 1.01), relative humidity (OR = 1.11) and temperature (OR = 0.13). Limitations Laboratory diagnosis was done at the batch level, with individual diagnosis based on clinical signs only. Conclusions This study confirms the multifactorial nature of S. suis-associated disease, with both environmental factors and factors related to the host involved in disease development. Controlling these factors may, therefore, help prevent the appearance of disease.Program for Innovative Global Prevention of Streptococcus suis (PIGSs), from program Horizon 2020 of the European Commission. Carlos Neila-Ibáñez was funded by the same project, Grant/Award Number: 727966info:eu-repo/semantics/publishedVersio

Remoción de carbono orgánico total en aguas residuales provenientes de laboratorio químico, empleando reactivo fenton

El tratamiento de efluentes residuales industriales representa un serio problema en la actualidad, ya que, por lo general, requiere de soluciones particulares (ad hoc). Este trabajo describe el uso del reactivo Fenton [reacción entre H2O2 y Fe(II)] en la remoción de materia orgánica medida como carbono orgánico total (COT), sobre un efluente proveniente de un laboratorio de análisis quími-co. Para dicho fin se evaluó el papel que desempeñan algunas de las condiciones de operación del proceso [concentraciones de H2O2 y Fe(II], temperatura y pH] sobre la remoción final de COT del efluente. Al mismo tiempo se desarrolló la optimización econó-mica del tratamiento, ajustando los datos experimentales a un modelo multivariable polinomial de segundo orden que representa el comportamiento del sistema con un R2 de 0.94. Experimentalmente, la mejor remoción encontrada fue de 88,8%, empleando [Fe(II)]0 = 50 mg/L; [H2O2]0 = 50 mM; pH = 2,8 y T = 80 oC, mientras que en el óptimo económico ([Fe(II)]0 = 36 mg/L; [H2O2]0 = 45,5 mM; pH= 2,6 y T = 20 °C) se alcanzó un 53,9%. Por otra parte, se evidenció que incluso en condiciones adversas para el reactivo Fenton (pH sobre 6) se consiguió una remoción de COT del orden de 41%. Se encontró que la concentración de H2O2 y la temperatura de reacción tienen los efectos más significativos tanto en la eficiencia del proceso como en el costo de operación, lo que las convier-te en variables a priorizar en la ejecución del tratamiento.Treating industrial wastewater represents a serious problem nowadays; it requires a strong understanding of the particular systems and (in most of cases) ad hoc solutions. This work describes the use of Fenton’s reagent (reaction between H2O2 and Fe(II)) for removing total organic carbon (TOC) from a particular chemical laboratory’s lab-scale batch reactor wastewater. Some operating variables (hydrogen peroxide and ferrous ion concentration, temperature and pH) were evaluated regarding final TOC removal. An economic optimisation was made by means of a second order polynomial model representing these variables’ behaviour regarding TOC removal (0.94 R2). The highest experimentally reached TOC removal was 88.8% at 50 mg/L [Fe(II)]0, 50 mM [H2O2]0 , pH=2.8 at 80oC, while 53.9% was obtained in optimised conditions, i.e. 36 mg/L [Fe(II)]0 , 45.5 mM [H2O2]0 , pH=2.6 at 20°C. It was found that the Fenton process could achieve 41% removal, even in adverse conditions (pH close to 6). It was noted from the analysis that both H2O2 concentration and temperature had a powerful effect on organic matter degradation efficiency, as well as on total treatment cost

Changes in Biomarkers of Redox Status in Saliva of Pigs after an Experimental Sepsis Induction

Saliva from pigs is gaining attention as an easy sample to obtain, being a source of biomarkers that can provide information on animal health and welfare. This study aimed to evaluate the changes that can occur in salivary biomarkers of the redox status of pigs with an experimentally induced sepsis. For that, the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of saliva (FRAS), Trolox equivalent antioxidant capacity (TEAC), advanced oxidation protein products (AOPP), ferrous oxidation-xylenol orange (FOX), peroxide activity (POX-Act), and reactive oxygen-derived compounds (d-ROMs) were measured in the saliva of pigs with experimentally induced sepsis by endotoxin lipopolysaccharide (LPS), non-septic inflammation induced by turpentine, and in healthy individuals before and after 3 h, 6 h, 24 h, and 48 h. AOPP, POX-Act, and d-ROMs in the sepsis group were higher than in the control from 3 h to 24 h after the inoculation. CUPRAC, FRAS, and TEAC were higher in sepsis than the control group at 24 h. These changes were of higher magnitude than those that occurred in the turpentine group. In conclusion, our findings reveal that sepsis produces changes in salivary biomarkers of redox status, which opens the possibility of using them as potential biomarkers in this species

Conocimiento y prácticas sobre eventos adversos, personal asistencial, Hospital san Juan de Dios, Cartago, Valle 2017

Los eventos adversos son las lesiones o complicaciones involuntarias que ocurren durante la atención en salud, los cuales son más atribuibles a esta; que a la enfermedad propia y que pueden conducir a la muerte, la incapacidad o al deterioro en el estado de salud del paciente, a la demora del alta, a la prolongación del tiempo de estancia hospitalaria y al incremento de los costos de no-calidad.Declaración: EL AUTOR-ESTUDIANTE, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto, la obra es de su exclusiva autoría y tiene la titularidad sobre la misma. PARÁGRAFO: en caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL ESTUDIANTE-AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la Fundación Universitaria del Área Andina actúa como un tercero de buena fe

Risk factors associated with Streptococcus suis cases on pig farms in Spain

Altres ajuts: acords transformatius de la UABBackground: Streptococcus suis can cause meningitis, polyarthritis and acute death in piglets. However, the risk factors associated with S. suis infection remain incompletely understood. Therefore, a longitudinal study was carried out, in which six batches from two Spanish pig farms with S. suis problems were repeatedly examined to determine possible risk factors. Methods: A prospective case-control study was conducted, and potential risk factors were evaluated using mixed-effects logistic regression models. The explanatory variables included: (a) concomitant pathogens; (b) biomarkers associated with stress, inflammation and oxidative status; (c) farm environmental factors; and (d) parity and S. suis presence in sows. Three models were built to study the effect of these variables, including two to assess the risk factors involved in the subsequent development of disease. Results: Risk factors for S. suis-associated disease included porcine reproductive and respiratory syndrome virus co-infection at weaning (odds ratio [OR] = 6.69), sow parity (OR = 0.71), haptoglobin level before weaning (OR = 1.01), relative humidity (OR = 1.11) and temperature (OR = 0.13). Limitations: Laboratory diagnosis was done at the batch level, with individual diagnosis based on clinical signs only. Conclusions: This study confirms the multifactorial nature of S. suis-associated disease, with both environmental factors and factors related to the host involved in disease development. Controlling these factors may, therefore, help prevent the appearance of disease

Role of Haptoglobin as a Marker of Muscular Improvement in Patients with Multiple Sclerosis after Administration of Epigallocatechin Gallate and Increase of Beta-Hydroxybutyrate in the Blood: A Pilot Study

Here, we report on the role of haptoglobin (Hp), whose expression depends on the synthesis of interleukin 6 (IL-6), related to the pathogenesis of multiple sclerosis (MS), as a possible marker of muscle improvement achieved after treatment with the polyphenol epigallocatechin gallate (EGCG) and an increase in the ketone body beta-hydroxybutyrate (BHB) in the blood. After 4 months of intervention with 27 MS patients, we observed that Hp does not significantly increase, alongside a significant decrease in IL-6 and a significant increase in muscle percentage. At the same time, Hp synthesis is considerably and positively correlated with IL-6 both before and after treatment; while this correlation occurs significantly reversed with muscle percentage before treatment, no correlation is evident after the intervention. These results seem to indicate that Hp could be a marker of muscle status and could be a diagnosis tool after therapeutic intervention in MS patients.Enfermerí

La socioafectividad de las personas con antecedentes delictivos: una aproximación desde un enfoque mixto.

Tablas, gráficasEl aumento de la delincuencia es un hecho que requiere ser estudiando desde diferentes dimensiones. Una de ellas es la indagación de la socioafectividad y el tipo de vínculo afectivo que pueden actuar como factores que predisponen o desencadenan esta conducta. El objetivo principal es realizar una descripción de los aspectos socioafectivos y el tipo de vínculo que presenta la población objeto de estudio. El enfoque del estudio es mixto y el diseño es descriptivo. Se realizó en la Fundación Manantial de Vida de la ciudad de Girardot, con participación de 30 hombres con edades entre 17 y 60 años. Las técnicas empleadas fueron el grupo reflexivo, la observación participante y la entrevista semi-estructurada. Se aplicó además el instrumento para medir los vínculos parentales PBI. En las actividades generadoras se trabajaron los siguientes temas: motivación, resiliencia, escucha activa, creatividad para la solución de problemas, autoestima, motivación, cooperación y trabajo en equipo, conciencia ética y moral, negociación y vínculo afectivoThe rise in crime is a fact that needs to be studied from different dimensions. One is the investigation of socioaffective and type of bonding that can act as factors that predispose or trigger this behavior. The main objective is to describe the socio-affective aspects and the type of bond that has the population under study. The focus of the study is mixed and design is descriptive. It was held at the Manantial de Vida Foundation of the city of Girardot, involving 30 men aged between 17 and 60 years. The techniques used were reflective group, participant observation and semi-structured interview. The instrument was applied to measure the GDP parental ties. Motivation, resilience, active listening, creativity for problem solving, self-esteem, motivation, cooperation and teamwork, ethics and moral awareness, negotiation and bonding: generating activities following topics are worked

Changes in a Comprehensive Profile of Saliva Analytes in Fattening Pigs during a Complete Productive Cycle : A Longitudinal Study

The aim of this study was to evaluate whether a panel of 29 salivary biomarkers of stress, immunity, inflammation, redox homeostasis and other physiological functions can change in healthy fattening pigs when monitoring the different phases of their productive cycle and can be influenced by various sources of variations such as gender and performance parameters. Several analytes showed changes due to the productive cycle, with a majority of the analytes showing higher values at lactation and at the beginning of nursery. Additionally, differences were seen due to sex. These differences can be related in some cases with performance parameters and should be taken into consideration for an appropriate interpretation of the analytes. A comprehensive panel of 29 salivary analytes was measured in fattening pigs to evaluate its possible changes along their productive cycle. The identification of those changes would allow a better interpretation of the results according to the productive phase of the animal. Saliva samples were obtained from 49 Large-White pigs (24 females, 25 males) in suckling phase, at the beginning and the end of the nursery phase, and at the beginning and the end of the growing phase. Several analytes changed according to the phase of the productive cycle, with most of the analytes showing higher values at lactation and at the beginning of nursery. Additionally, differences were seen due to sex. When possible relations between performance parameters and analytes were evaluated, significant positive but weak relationships were found between weight at birth and salivary γ-glutamyl transferase, and between back-fat thickness and salivary lactate dehydrogenase. In conclusion, differences in the values of salivary analytes can be found in fattening pigs depending on the productive phase and sex of the animals