Genome-wide profiling of uncapped mRNA

Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRNA synthesis and degradation. In this chapter, we describe a method to profile these uncapped mRNAs at the genome scale

Mimicry technology : a versatile tool for small RNA suppression

A decade ago the discovery of the target mimicry regulatory process on the activity of a mature microRNA (miRNA) enabled for the first time the customized attenuation of miRNA activity in plants. That powerful technology was named MIMIC and was based on engineering the IPS1 long noncoding transcript to become complementary to the miRNA under study. In order to avoid IPS1 degradation, the predicted miRNA-mediated cleavage site was interrupted by three additional nucleotides giving rise to the so-called MIMIC decoy. Since then, MIMIC technology has been used in several plant species and in basic and translational research. We here provide a detailed guide to produce custom-designed MIMIC decoys to facilitate the study of sRNA functions in plants

Heavy Squarks at the LHC

The LHC, with its seven-fold increase in energy over the Tevatron, is capable of probing regions of SUSY parameter space exhibiting qualitatively new collider phenomenology. Here we investigate one such region in which first generation squarks are very heavy compared to the other superpartners. We find that the production of these squarks, which is dominantly associative, only becomes rate-limited at mSquark > 4(5) TeV for L~10(100) fb-1. However, discovery of this scenario is complicated because heavy squarks decay primarily into a jet and boosted gluino, yielding a dijet-like topology with missing energy (MET) pointing along the direction of the second hardest jet. The result is that many signal events are removed by standard jet/MET anti-alignment cuts designed to guard against jet mismeasurement errors. We suggest replacing these anti-alignment cuts with a measurement of jet substructure that can significantly extend the reach of this channel while still removing much of the background. We study a selection of benchmark points in detail, demonstrating that mSquark= 4(5) TeV first generation squarks can be discovered at the LHC with L~10(100)fb-1

PROTOCOLO DE USO Y MANTENIMIENTO DEL RESERVORIO VENOSO SUBCUTÁNEO

Objetive: To establish a standardized care plan for carrier patients of venous reservoir in primary care. Material and Method: A revision of articles related to subcutaneous venous reservoir use and management was carried out in various data base. The articles were revised two at a time and classified by affinity and technical scientific quality. Later on, the authors, by consent, determine a standardized care plan for this kind of patients, as well as a connection of the plan to the different work lines (assistance process, health programs, shared performance guide and protocols) existing in the Public Health System of Andalucia, Spain (SSPA). Results: Once the theory phase of making the care plan was completed, the same one was carried out with the related cases in one of the health basic areas. Its adequacy and acceptance by professionals and patients was evaluated. At present, the mentioned plan it has been sent to the care board for its assessment, if necessary, to the rest of basic areas of the health district. Conclusions: In accordance with the results we have found in readings, determining standardized care plans is an excellent tool in care: It facilitates the decision making, decreases the variability, and improves the registry and the professional and users satisfaction.Objetivos: Establecer un plan de cuidados estandarizado para los pacientes portadores de reservorio venoso en atención primaria. Material y método: Se realizó una revisión de la literatura de artículos relacionados con uso y manejo del reservorio venoso subcutáneo en distintas bases de datos. Los artículos fueron revisados por pares y filtrados por afinidad y calidad científico técnica. Posteriormente los autores, mediante técnicas de consenso, determinaron el plan de cuidados estandarizado para este tipo de pacientes así como la conexión de éste con las diferentes líneas de trabajo (procesos asistenciales, programas de salud, guías de actuación compartida y protocolos) existentes en el sistema sanitario público andaluz (SSPA). Resultados: Concluida la fase teórica de elaboración del plan de cuidados, se pilotó el mismo con los casos incidentes en una de las zonas básicas de salud, viendo su adecuación y aceptación por profesionales y pacientes. En la actualidad dicho plan se ha remitido a la dirección de cuidados para su valoración y difusión, si procede, al resto de zonas básicas del distrito sanitario Conclusiones: En concordancia con los resultados encontrados en la literatura, la determinación de planes de cuidados estandarizados constituye una herramienta de excelencia en los cuidados que: facilita la toma de decisiones, disminuye la variabilidad, mejora el registro y la satisfacción de profesionales y usuarios

Emerging Non-Anomalous Baryonic Symmetries in the AdS_5/CFT_4 Correspondence

We study the breaking of baryonic symmetries in the AdS_5/CFT_4 correspondence for D3 branes at Calabi-Yau three-fold singularities. This leads, for particular VEVs, to the emergence of non-anomalous baryonic symmetries during the renormalization group flow. We claim that these VEVs correspond to critical values of the B-field moduli in the dual supergravity backgrounds. We study in detail the C^3/Z_3 orbifold, the cone over F_0 and the C^3/Z_5 orbifold. For the first two examples, we study the dual supergravity backgrounds that correspond to the breaking of the emerging baryonic symmetries and identify the expected Goldstone bosons and global strings in the infra-red. In doing so we confirm the claim that the emerging symmetries are indeed non-anomalous baryonic symmetries.Comment: 65 pages, 15 figures;v2: minor changes, published versio

Quantum biology on the edge of quantum chaos

We give a new explanation for why some biological systems can stay quantum coherent for long times at room temperatures, one of the fundamental puzzles of quantum biology. We show that systems with the right level of complexity between chaos and regularity can increase their coherence time by orders of magnitude. Systems near Critical Quantum Chaos or Metal-Insulator Transition (MIT) can have long coherence times and coherent transport at the same time. The new theory tested in a realistic light harvesting system model can reproduce the scaling of critical fluctuations reported in recent experiments. Scaling of return probability in the FMO light harvesting complex shows the signs of universal return probability decay observed at critical MIT. The results may open up new possibilities to design low loss energy and information transport systems in this Poised Realm hovering reversibly between quantum coherence and classicality

The Beta Ansatz: A Tale of Two Complex Structures

Brane tilings, sometimes called dimer models, are a class of bipartite graphs on a torus which encode the gauge theory data of four-dimensional SCFTs dual to D3-branes probing toric Calabi-Yau threefolds. An efficient way of encoding this information exploits the theory of dessin d’enfants, expressing the structure in terms of a permutation triple, which is in turn related to a Belyi pair, namely a holomorphic map from a torus to a P1 with three marked points. The procedure of a-maximization, in the context of isoradial embeddings of the dimer, also associates a complex structure to the torus, determined by the R-charges in the SCFT, which can be compared with the Belyi complex structure. Algorithms for the explicit construction of the Belyi pairs are described in detail. In the case of orbifolds, these algorithms are related to the construction of covers of elliptic curves, which exploits the properties of Weierstraß elliptic functions. We present a counter example to a previous conjecture identifying the complex structure of the Belyi curve to the complex structure associated with R-charges