Dynamical transitions in a pollination--herbivory interaction

Plant-pollinator associations are often seen as purely mutualistic, while in reality they can be more complex. Indeed they may also display a diverse array of antagonistic interactions, such as competition and victim--exploiter interactions. In some cases mutualistic and antagonistic interactions are carried-out by the same species but at different life-stages. As a consequence, population structure affects the balance of inter-specific associations, a topic that is receiving increased attention. In this paper, we developed a model that captures the basic features of the interaction between a flowering plant and an insect with a larval stage that feeds on the plant's vegetative tissues (e.g. leaves) and an adult pollinator stage. Our model is able to display a rich set of dynamics, the most remarkable of which involves victim--exploiter oscillations that allow plants to attain abundances above their carrying capacities, and the periodic alternation between states dominated by mutualism or antagonism. Our study indicates that changes in the insect's life cycle can modify the balance between mutualism and antagonism, causing important qualitative changes in the interaction dynamics. These changes in the life cycle could be caused by a variety of external drivers, such as temperature, plant nutrients, pesticides and changes in the diet of adult pollinators. Abstract Keywords: mutualism, pollination, herbivory, insects, stage-structure, oscillationsComment: 20 pages, 7 main figures, 2 appendix figure

Limited Access to Food and Physiological Trade-Offs in a Long-Distance Migrant Shorebird. II. Constitutive Immune Function and the Acute-Phase Response

In response to unbalanced energy budgets, animals must allocate resources among competing physiological systems to maximize fitness. Constraints can be imposed on energy availability or energy expenditure, and adjustments can be made via changes in metabolism or trade-offs with competing demands such as body-mass maintenance and immune function. This study investigates changes in constitutive immune function and the acute-phase response in shorebirds (red knots) faced with limited access time to food. We separated birds into two experimental groups receiving either 6 h or 22 h of food access and measured constitutive immune function. After 3 wk, we induced an acute-phase response, and after 1 wk of recovery, we switched the groups to the opposite food treatment and measured constitutive immune function again. We found little effect of food treatment on constitutive immune function, which suggests that even under resource limitation, a baseline level of immune function is maintained. However, birds enduring limited access to food suppressed aspects of the acute-phase response (decreased feeding and mass loss) to maintain energy intake, and they downregulated thermoregulatory adjustments to food treatment to maintain body temperature during simulated infection. Thus, under resource-limited conditions, birds save energy on the most costly aspects of immune defense.</p

Implications of the 2019–2020 megafires for the biogeography and conservation of Australian vegetation

Australia's 2019–2020 'Black Summer' bushfires burnt more than 8 million hectares of vegetation across the south-east of the continent, an event unprecedented in the last 200 years. Here we report the impacts of these fires on vascular plant species and communities. Using a map of the fires generated from remotely sensed hotspot data we show that, across 11 Australian bioregions, 17 major native vegetation groups were severely burnt, and up to 67–83% of globally significant rainforests and eucalypt forests and woodlands. Based on geocoded species occurrence data we estimate that >50% of known populations or ranges of 816 native vascular plant species were burnt during the fires, including more than 100 species with geographic ranges more than 500 km across. Habitat and fire response data show that most affected species are resilient to fire. However, the massive biogeographic, demographic and taxonomic breadth of impacts of the 2019–2020 fires may leave some ecosystems, particularly relictual Gondwanan rainforests, susceptible to regeneration failure and landscape-scale decline

Advancing DNA barcoding and metabarcoding applications for plants requires systematic analysis of herbarium collections-an Australian perspective

Building DNA barcode databases for plants has historically been ad hoc, and often with a relatively narrow taxonomic focus. To realize the full potential of DNA barcoding for plants, and particularly its application to metabarcoding for mixed-species environmental samples, systematic sequencing of reference collections is required using an augmented set of DNA barcode loci, applied according to agreed data generation and analysis standards. The largest and most complete reference collections of plants are held in herbaria. Australia has a globally significant flora that is well sampled and expertly curated by its herbaria, coordinated through the Council of Heads of Australasian Herbaria. There exists a tremendous opportunity to provide a comprehensive and taxonomically robust reference database for plant DNA barcoding applications by undertaking coordinated and systematic sequencing of the entire flora of Australia utilizing existing herbarium material. In this paper, we review the development of DNA barcoding and metabarcoding and consider the requirements for a robust and comprehensive system. We analyzed the current availability of DNA barcode reference data for Australian plants, recommend priority taxa for database inclusion, and highlight future applications of a comprehensive metabarcoding system. We urge that large-scale and coordinated analysis of herbarium collections be undertaken to realize the promise of DNA barcoding and metabarcoding, and propose that the generation and curation of reference data should become a national investment priority

Genetic diversity and structure of the Australian flora

Aim To investigate the relationships between species attributes and genetic parameters in Australian plant species and to determine the associations in relation to predictions from population theory and previous global analyses. Location Continent of Australia. Methods We assembled a dataset of all known population genetic analyses of Australian plants based on neutral markers and catalogued them according to key species attributes, including range, abundance, range disjunction, biome and growth form; and genetic parameters, mean number of alleles per locus, observed and expected heterozygosity and population differentiation. We determined relationships between species attributes and genetic parameters using a maximum‐likelihood, multimodel inference approach. Results We found many associations that were consistent with predictions. Species attributes with greatest effect on genetic diversity were range size, growth form, abundance and biome. The most important attributes influencing genetic differentiation were range disjunction and abundance. We found unexpected results in the effects of biome and growth form on genetic diversity, with greater diversity in the eastern biome of Australia, and lower diversity in shrubs compared to trees. Main conclusions Our analysis of genetic diversity of Australian plants showed associations consistent with predictions based on population genetics theory, with strong effects of range size, abundance and growth form. We identified a striking effect of range disjunction on population genetic differentiation, an effect that has received little attention in the literature. We also found some notable differences to global predictions, which were most likely explained by confounding effects across variables. This highlights that caution is needed when extrapolating trends from global analyses to regional floras. Identifying associations between species attributes and patterns of genetic diversity enables broadscale predictions to facilitate the inclusion of genetic considerations into conservation decision‐making.This research was supported by the joint resources of CSIRO and the Western Australian Department of Parks and Wildlif

Population Genetics of Duplicated Alternatively Spliced Exons of the Dscam Gene in Daphnia and Drosophila

In insects and crustaceans, the Down syndrome cell adhesion molecule (Dscam) occurs in many different isoforms. These are produced by mutually exclusive alternative splicing of dozens of tandem duplicated exons coding for parts or whole immunoglobulin (Ig) domains of the Dscam protein. This diversity plays a role in the development of the nervous system and also in the immune system. Structural analysis of the protein suggested candidate epitopes where binding to pathogens could occur. These epitopes are coded by regions of the duplicated exons and are therefore diverse within individuals. Here we apply molecular population genetics and molecular evolution analyses using Daphnia magna and several Drosophila species to investigate the potential role of natural selection in the divergence between orthologs of these duplicated exons among species, as well as between paralogous exons within species. We found no evidence for a role of positive selection in the divergence of these paralogous exons. However, the power of this test was low, and the fact that no signs of gene conversion between paralogous exons were found suggests that paralog diversity may nonetheless be maintained by selection. The analysis of orthologous exons in Drosophila and in Daphnia revealed an excess of non-synonymous polymorphisms in the epitopes putatively involved in pathogen binding. This may be a sign of balancing selection. Indeed, in Dr. melanogaster the same derived non-synonymous alleles segregate in several populations around the world. Yet other hallmarks of balancing selection were not found. Hence, we cannot rule out that the excess of non-synonymous polymorphisms is caused by segregating slightly deleterious alleles, thus potentially indicating reduced selective constraints in the putative pathogen binding epitopes of Dscam

Ecology and evolution of mutualistic networks

Mutualistische interacties komen overal voor in de natuur en zijn cruciaal voor de overleving en reproductie van veel soorten. Sinds kort wordt pas erkend dat mutualistische interaties belangrijk zijn voor de verbindingen tussen soorten, voor de modulatie van ecosysteemfunctionering en voor het behoud van biodiversiteit. Mutualistische gemeenschappen, zoals plant-bestuiver gemeenschappen, kunnen worden beschreven als netwerken van interacties tussen mutualistiche partners (bijv. planten en dieren). Met behulp van deze aanpak hebben vele studies hun licht kunnen schijnen op de structuur van mutualistische gemeenschappen en hun eco-evolutionaire patronen. De vraag is: welke ecologische en evolutionaire processen liggen ten grondslag aan de opbouw van mutualistische gemeenschappen? We hebben wiskundige modellen en simulaties ontwikkeld en deze gecombineerd met data analyse om deze vragen te kunnen beantwoorden voor een scala aan mutualistische systemen, waarbij we vooral gefocust hebben op mutualistische plant-dier interacties. We hebben gevonden dat spatio-temporele variabiliteit de belangrijkste factor is van de mutualistische netwerk structuur. De combinatie van ruimtelijke structuur en de variabiliteit in de timing van interacties tussen soorten (fenologie) is wat het meest de mutualistische netwerkstructuur bepaald. De stabiliteit en diversiteit van deze gemeenschappen hangt echter af van de balans van deze processen met andere typen ecologische interacties en de life-history van de soort. Bovendien blijkt het dat veel voorkomende evolutionaire patronen in mutualistiche netwerken, zoals evolutionaire convergentie en complementariteit, lijken te ontstaan uit simpele ecologische (bijv. dispersielimitatie en ecologische drift) en evolutionaire (bijv. mutatie, recombinatie en genetische drift) processen. We concluderen dat: 1) neutrale eco-evolutionare processen niet moeten worden genegeerd bij het bestuderen van de evolutie van ecologische netwerken en 2) ruimtelijke processen (dispersielimitatie, immigratie) en fenologie van essentieel belang zijn voor de samenstelling van mutualistische netwerken, en waarschijnlijk voor ecologische netwerken in het algemeen.